Gene Expression Profiles During Tuberous Root Development

Chapter
Part of the Compendium of Plant Genomes book series (CPG)

Abstract

Great variation in root size and shape can be observed in local and commercial varieties of radish. At present, little is known about the underlying molecular mechanisms that determine the size and shape of thickening roots not only in radish but also in other root crops. This chapter first summarizes the properties of vegetative growth, developmental patterns, and anatomical structures of radish tuberous roots. Next, genes expressed in radish roots, particularly in the key developmental stages and proliferating tissues during tuberization, are presented based on several studies using next-generation sequencing data. Transcriptome analyses revealed that genes and pathways related to starch and sucrose metabolism are particularly activated in thickening roots and their cell proliferating tissues. The examinations of expression patterns of genes involved in the sucrose metabolism pathway during root development revealed that genes encoding sucrose synthase, a key enzyme involved in sucrose cleavage, shows consistently high expression patterns in radish thickening roots, whereas low expression was observed in young seedling roots, suggesting that this enzyme plays a critical role in radish root tuberization.

Keywords

Differentially expressed genes Radish Raphanus Root tuberization Sucrose metabolism Sucrose synthase Transcriptome 

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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  1. 1.Tokyo University of AgricultureTokyoJapan

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