Economic and Academic Importance of Radish

Part of the Compendium of Plant Genomes book series (CPG)


Radish is important as a root vegetable, a leafy vegetable, a fruit vegetable, an oil crop, and also as a cover plant. The economic importance and characteristics of radish differ between the East and the West of the world. In the East, there are radish cultivars having large roots with various shapes called “Asian big radish” and those grown for production of immature pods or oil seeds, whereas radish is a small vegetable grown within one month in the West. Asian big radish is expected to eventually become popular in the West. Radish belongs to the genus Raphanus, but is similar to the Brassica species except for the shape of pods and seeds. Despite their similarities, the order of genes in chromosomes is quite different between Raphanus and Brassica. Radish genome sequences have been published from three groups using similar cultivars, and therefore, collaboration for combining sequence data is considered to be effective for determination of more reliable genome sequences. Some radish lines have high salt tolerance and disease resistance different from Brassica crops. Radish also has a characteristic glucosinolate composition. Since radish can be crossed with Brassica species, it is also important as a genetic resource for Brassica crop breeding.


  1. Ahuja KL, Singh H, Raheja RK, Labana KS (1987) The oil content and fatty acid composition of various genotypes of cauliflower, turnip and radish. Plant Foods Hum Nutr 37:33–40CrossRefGoogle Scholar
  2. Akaba M, Kaneko Y, Hatakeyama K, Ishida M, Bang SW, Matsuzawa Y (2009) Identification and evaluation of clubroot resistance of radish chromosome using a Brassica napus–Raphanus sativus monosomic addition line. Breed Sci 59:203–206CrossRefGoogle Scholar
  3. Arumuganathan K, Earle ED (1991) Nuclear DNA content of some important species. Plant Mol Biol Rep 9:208–218Google Scholar
  4. Barillari J, Cervellati R, Paolini M, Tatibouet A, Rollin P, Iori R (2005) Isolation of 4-methylthio-3-butenyl glucosinolate from Raphanus sativus sprouts (kaiware daikon) and its redox properties. J Agric Food Chem 53:9890–9896Google Scholar
  5. Bennetzen J, Freeling M (1997) The unified grass genome: synergy in synteny. Genome Res 7:301–306CrossRefPubMedGoogle Scholar
  6. Cho MA, Min SR, Ko SM, Liu JR, Choi PS (2008) Agrobacterium-mediated genetic transformation of radish (Raphanus sativus L.). Plant Biotechnol 25:205–208CrossRefGoogle Scholar
  7. Gomez-Campo C (1980) Morphology and morpho-taxonomy of the tribe Brassiceae. In: Tsunoda S et al (eds) Brassca crops and wild allies. Japan Scientific Societies Press, Tokyo, pp 3–31Google Scholar
  8. Harberd DJ, McArthur ED (1980) Meiotic analysis of some species and genus hybrids in the Brassiceae. In: Tsunoda S et al (eds) Brassca crops and wild allies. Japan Scientific Societies Press, Tokyo, pp 65–87Google Scholar
  9. Hashida T, Nakatsuji R, Budahn H, Schrader O, Peterka H, Fujimura T, Kubo N, Hirai M (2013) Construction of a chromosome-assigned, sequence-tagged linkage map for the radish, Raphanus sativus L. and QTL analysis of morphological traits. Breed Sci 63:218–226CrossRefPubMedPubMedCentralGoogle Scholar
  10. Inaba R, Nishio T (2002) Phylogenetic analysis of Brassiceae based on the nucleotide sequences of the S-locus related gene, SLR1. Theor Appl Genet 105:1159–1165CrossRefPubMedGoogle Scholar
  11. Ishida M, Kakizaki T, Morimitsu Y, Ohara T, Hatakeyama K, Yoshiaki H, Kohori J, Nishio T (2015) Novel glucosinolate composition lacking 4-methylthio-3-butenyl glucosinolate in Japanese white radish (Raphanus sativus L.). Thoer Appl Genet 128:2037–2046CrossRefGoogle Scholar
  12. Jeong Y-M, Kim N, Ahn B, Oh M, Chung W-H, Chung H, Jeong S, Lim K-B, Hwang Y-J, Kim G-B, Baek S, Choi S-B, Hyung D-J, Lee S-W, Sohn S-H, Kwon S-J, Jin M, Seol Y-J, Chae W, Choi K, Park B-S, Yu H-J, Mun J-H (2016) Elucidating the triplicated ancestral genome structure of radish based on chromosome-level comparison with the Brassica genomes. Theor Appl Genet 129:1357–1372CrossRefPubMedGoogle Scholar
  13. Johnston JS, Pepper AE, Hall AE, Chen ZJ, Hodnett G, Drabek J, Lopez R, Price HJ (2005) Evolution of genome size in Brassicaceae. Ann Bot 95:229–235Google Scholar
  14. Kakizaki T, Kitashiba H, Zou Z, Li F, Fukino N, Ohara T, Nishio T, Ishida M (2017) A 2-oxoglutarate-dependent dioxygenase mediates the biosynthesis of glucoraphasatin in radish. Plant Physiol 173:1583–1593Google Scholar
  15. Karpechenko GD (1924) Hybrids of Raphanus sativus L. x Brassica oleracea L. J Genet 14:375–396Google Scholar
  16. Kamei A, Tsuro M, Kubo N, Hayashi T, Wang N, Fujimura T, Hirai M (2010) QTL mapping of clubroot resistance in radish (Raphanus sativus L.). Theor Appl Genet 120:1021–1027CrossRefPubMedGoogle Scholar
  17. Kitashiba H, Li F, Hirakawa H, Kawanabe T, Zou Z, Hasegawa Y, Tonosaki K, Shirasawa S, Fukushima A, Yokoi S, Takahata Y, Kakizaki T, Ishida M, Okamoto S, Sakamoto K, Shirasawa K, Tabata S, Nishio T (2014) Draft sequences of the radish (Raphanus sativus L.) genome. DNA Res 21:481–490CrossRefPubMedPubMedCentralGoogle Scholar
  18. Koizuka N, Imai R, Fujimoto H, Hayakawa T, Kimura Y, Kohno-Murase J, Sakai T, Kawasaki S, Imamura J (2003) Genetic characterization of a pentatricopeptide repeat protein gene, orf587, that restores fertility in the cytoplasmic male-sterile Kosena radish. Plant J 34:407–415CrossRefPubMedGoogle Scholar
  19. Lelivelt CLC, Lang W, Dolstra O (1993) Intergeneric crosses for the transfer of resistance to the beet cyst nematode from Raphanus sativus to Brassica napus. Euphytica 58:111–120CrossRefGoogle Scholar
  20. Li F, Hasegawa Y, Saito M, Shirasawa S, Fukushima A, Ito T, Fujii H, Kishitani S, Kitashiba H, Nishio T (2011) Extensive chromosome homoeology among Brassiceae species were revealed by comparative genetic mapping with high-density EST-based SNP markers in radish (Raphanus sativus L.). DNA Res 18:401–411CrossRefPubMedPubMedCentralGoogle Scholar
  21. Lim SH, Cho HJ, Lee SJ, Cho YH, Kim BD (2002) Identification and classification of S haplotypes in Raphanus sativus by PCR-RFLP of the S locus glycoprotein (SLG) gene and the S locus receptor kinase (SRK) gene. Theor Appl Genet 104:1253–1262CrossRefPubMedGoogle Scholar
  22. McCallum CM, Comai L, Greene EA, Henikoff S (2000) Targeted screening for induced mutations. Nat Biotechnol 18:455–457CrossRefPubMedGoogle Scholar
  23. Mitsui Y, Shimomura M, Komatsu K, Namiki N, Shibata-Hatta M, Imai M, Katayose Y, Mukai Y, Kanamori H, Kurita K, Kagami T, Wakatsuki A, Ohyanagi H, Ikawa H, Minaka N, Nakagawa K, Shiwa Y, Sasaki T (2015) The radish genome and comprehensive gene expression profile of tuberous root formation and development. Sci Rep 5:10835CrossRefPubMedPubMedCentralGoogle Scholar
  24. Mizushima U (1980) Genome analysis in Brasica and allied genera. In: Tsunoda S et al (eds) Brassca crops and wild allies. Japan Scientific Societies Press, Tokyo, pp 89–106Google Scholar
  25. Mun J-H, Chung H, Chung W-H, Oh M, Jeong Y-M, Kim N, Ahn BO, Park B-S, Park S, Lim K-B, Hwang Y-J, Yu H-J (2015) Construction of a reference genetic map of Raphanus sativus based on genotyping by whole-genome resequencing. Theor Appl Genet 128:259–272CrossRefPubMedGoogle Scholar
  26. Nasu S, Kitashiba H, Nishio T (2012) “Na-no-hana Project” for recovery from the Tsunami disaster by producing salinity-tolerant oilseed rape lines: Selection of salinity-tolerant lines of Brassica crops. J Integr Field Sci 9:33–37Google Scholar
  27. Niikura S, Matsuura S (1998) Identification of self-incompatibility alleles (S) by PCR-RFLP in radish (Raphanus sativus L.). Euphytica 102:379–384CrossRefGoogle Scholar
  28. Niikura S, Matsuura S (2000) Genetic analysis of the reaction level of self-incompatibility to a 4% CO2 gas treatment in the radish (Raphanus sativus L.). Theor Appl Genet 101:1189–1193CrossRefGoogle Scholar
  29. Nishio T, Kusaba M, Watanabe M, Hinata K (1996) Registration of S alleles in Brassica campestris L by the restriction fragment sizes of SLGs. Theor Appl Genet 92:388–394CrossRefPubMedGoogle Scholar
  30. Okamoto S, Sato Y, Sakamoto K, Nishio T (2004) Distribution of similar self-incompatibility (S) haplotypes in different genera, Raphanus and Brassica. Sex Plant Reprod 17:33–39CrossRefGoogle Scholar
  31. Ozeki Y (2010) Study on the relationship between the anthocyanin molecular species and color phenotype of roots in the inbred lines of red radish (Raphanus sativus L.). Japan Food Chem Res Found Res Rep 16:33–39 (in Japanese with English summary)Google Scholar
  32. Papi A, Orlandi M, Bartolini G, Barillari J, Iori R, Paolini M, Ferroni F, Fumo MG, Pedulli GF, Velgimigli L (2008) Cytotoxic and antioxidant activity of 4-methylthio-3-butenyl isothiocyanate from Raphanus sativus L. (kaiware daikon) sprouts. J Agric Food Chem 56:875–883CrossRefPubMedGoogle Scholar
  33. Park B-J, Liu Z, Kanno A, Kameya T (2005) Transformation of radish (Raphanus sativus L.) via sonication and vacuum infiltration of germinated seeds with Agrobacterium harboring a group 3 LEA gene from B. napus. Plant Cell Rep 24:494–500CrossRefPubMedGoogle Scholar
  34. Park NI, Xu H, Li X, Jang IH, Park S, Ahn GH, Lim YP, Kim SJ, Park SU (2011) Anthocyanin accumulation and expression of anthocyanin biosynthetic genes in radish (Raphanus sativus). J Agric Food Chem 59:6034–6039CrossRefPubMedGoogle Scholar
  35. Peters SA, Bargsten JW, Szinay D, van de Belt J, Visser RGF, Bai Y, de Jong H (2012) Structural homology in the Solanaceae: analysis of genomic regions in support of synteny studies in tomato, potato and pepper. Plant J 71:602–614CrossRefPubMedGoogle Scholar
  36. Sakamoto K, Kusaba M, Nishio T (1998) Polymorphism of the S-locus glycoprotein gene (SLG) and the S-locus related gene (SLR1) in Raphanus sativus L. and self-incompatible ornamental plants in the Brassicaceae. Mol Gen Genet 258:397–403CrossRefPubMedGoogle Scholar
  37. Shen D, Shu H, Huang M, Zheng Y, Li X, Fei Z (2013) RadishBase: a database for genomics and genetics of radish. Plant Cell Physiol 54(1–6):e3CrossRefPubMedGoogle Scholar
  38. Shirasawa K, Oyama M, Hirakawa H, Sato S, Tabata S, Fujioka T, Kimizuka-Takagi C, Sasamoto S, Watanabe A, Kato M, Kishida Y, Kohara M, Takahashi C, Tsuruoka H, Wada T, Sakai T, Isobe S (2011) An EST-SSR linkage map of Raphanus sativus and comparative genomics of the Brassicaceae. DNA Res 18:221–232CrossRefPubMedPubMedCentralGoogle Scholar
  39. Shishu KIP (2009) Inhibition of cooked food-induced mutagenesis by dietary constituents: comparison of two natural isothiocyanates. Food Chem 112:977–981CrossRefGoogle Scholar
  40. Takahata Y, Komatsu H, Kaizuma N (1996) Microspore culture of radish (Raphanus sativus L.): influence of genotype and culture conditions on embryogenesis. Plant Cell Rep 16:163–166PubMedGoogle Scholar
  41. Terasawa Y (1932) Tetraploid Bastarde von Brassica chinensis L. x Raphanus sativus L. Jpn J Genet 7:183–185 (In Japanese)Google Scholar
  42. Tonosaki K, Michiba K, Bang SW, Kitashiba H, Kaneko Y, Nishio T (2013) Genetic analysis of hybrid seed formation ability of Brassica rapa in intergeneric crossings with Raphanus sativus. Theor Appl Genet 126:837–846 Google Scholar
  43. Tsuro M, Suwabe K, Kubo N, Matsumoto S, Hirai M (2008) Mapping of QTLs controlling root shape and red pigmentation in radish, Raphanus sativus L. Breed Sci 58:55–61CrossRefGoogle Scholar
  44. Warwick SI, Black LD (1997) Phylogenetic implications of chloroplast DNA restriction site variation in subtribes Raphaninae and Cakilinae (Brassicaceae, tribe Brassiceae). Can J Bot 75:960–973CrossRefGoogle Scholar
  45. Warwick SI, Hall JC (2009) Phylogeny of Brassica and wild relatives. In: Gupta SK (ed) Biology and breeding of crucifers. CRC Press, London, pp 19–36Google Scholar
  46. Warwick SI, Sauder CA (2005) Phylogeny of tribe Brassiceae (Brassicaceae) based on chloroplast restriction site polymorphisms and nuclear ribosomal internal transcribed spacer and chloroplast trnL intron sequences. Can J Bot 83:467–483CrossRefGoogle Scholar
  47. Weil RR, Kremen A (2007) Thinking across and beyond disciplines to make cover crops pay. J Sci Food Agric 87:551–557CrossRefGoogle Scholar
  48. Yamagishi H, Terachi T (1996) Molecular and biological studies on male-sterile cytoplasm in the Cruciferae III. Distributtion of Ogura-type cytoplasm among Japanese wild radishes and Asian radish cultivars. Theor Appl Genet 93:325–332CrossRefPubMedGoogle Scholar
  49. Zou Z, Ishida M, Li F, Kakizaki T, Suzuki S, Kitashiba H, Nishio T (2013) QTL analysis using SNP markers developed by next-generation sequencing for identification of candidate genes controlling 4-methylthio-3-butenyl glucosinolate contents in roots of radish, Raphanus sativus L. PLoS ONE 8:e53541CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  1. 1.Graduate School of Agricultural ScienceTohoku UniversityAoba-ku, SendaiJapan

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