High-Throughput Sequencing of the Paired Human Immunoglobulin Heavy and Light Chain Repertoire

  • Brandon DeKosky
Part of the Springer Theses book series (Springer Theses)


Currently existing immune repertoire sequencing technologies yield data on only one of the two chains of immune receptors [1, 2, 3]. Sequence analysis of VH:VL pairs is therefore currently performed by microtiter-well sorting of individual B cells followed by single-cell RT-PCR (scRT-PCR) and Sanger sequencing [3−10]; however at most a few hundred VH:VL pairs (a number dwarfed by the enormous size of the human antibody repertoire) are identified via scRT-PCR [6−9].


Tetanus Toxoid Microwell Array Lithium Dodecyl Sulfate Gene Family Usage Light Chain Transcript 
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© Springer International Publishing AG 2017

Authors and Affiliations

  1. 1.Department of Chemical and Petroleum Engineering, Department of Pharmaceutical ChemistryThe University of KansasLawrenceUSA

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