Understanding the Mycorrhiza-Nanoparticles Interaction

  • Avinash Ingle
  • Dnyaneshwar Rathod
  • Ajit Varma
  • Mahendra RaiEmail author


Arbuscular mycorrhizal fungi (AMF) always associate with the roots of higher plants and form a mutualistic symbiosis with the roots of over 90% of plant species, including forest trees, wild grasses, and many crops. Recently, considerable efforts were put to revolutionize agricultural systems through the applications of nanotechnology in various ways. Nanoparticles exploited for plant growth promotion showed the controversial opinions. Similarly, the interaction of various nanoparticles with mycorrhizal fungi found to influence its growth and showed both positive and negative effects. Some of the nanoparticles helps in colonization of AMF, whereas some negatively affects the colonization. Therefore, understanding the exact mechanism of interaction between AMF and nanoparticles is necessary.

Hence, in this book chapter, we have focused on the influence of different nanoparticles on the growth of AMF. Moreover, we have also discussed the role of nanoparticle in plant growth promotion.



Ajit Varma is thankful to DBT for partial funding and DST for providing confocal microscope. MKR thankfully acknowledges the financial help rendered by UGC, New Delhi, under Special Assistance Programme (DRS-I).


  1. Arora S, Sharma P, Kumar S, Nayan R, Khanna PK, Zaidi MGH (2012) Gold-nanoparticle induced enhancement in growth and seed yield of Brassica juncea. Plant Growth Regul 66:303–310CrossRefGoogle Scholar
  2. Aziz N, Pandey R, Barman I, Prasad R (2016) Leveraging the attributes of Mucor hiemalis-derived silver nanoparticles for a synergistic broad-spectrum antimicrobial platform. Front Microbiol 7:1984. doi: 10.3389/fmicb.2016.01984 CrossRefPubMedPubMedCentralGoogle Scholar
  3. Bao-shan L, Shao-qi D, Chun-hui L, Li-jun F, Shu-chun Q, Min Y (2004) Effect of TMS (nanostructured silicon dioxide) on growth of Changbai larch seedlings. J Forest Res 15:138–140CrossRefGoogle Scholar
  4. Barrena R, Casals E, Colón J, Font X, Sánchez A, Puntes V (2009) Evaluation of the ecotoxicity of model nanoparticles. Chemosphere 75:850–857CrossRefPubMedGoogle Scholar
  5. Berruti A, Lumini E, Balestrini R, Bianciotto V (2016) Arbuscular mycorrhizal fungi as natural biofertilizers: let’s benefit from past successes. Front Microbiol 6:1559. doi: 10.3389/fmicb.2015.01559 CrossRefPubMedPubMedCentralGoogle Scholar
  6. Bonfante P, Genre A (2010) Mechanisms underlying beneficial plant-fungus interactions in mycorrhizal symbiosis. Nat Commun 1:48. doi: 10.1038/ncomms1046 CrossRefPubMedGoogle Scholar
  7. Boonyanitipong P, Kumar P, Kositsup B, Baruah S, Dutta J (2011). Effects of zinc oxide nanoparticles on roots of rice Oryza Sativa L. In: Proceeding of international conference on Environment and BioScience, vol 21. IACSIT Press, Singapore, pp 172–176Google Scholar
  8. Brundrett MC (2002) Coevolution of roots and mycorrhizas of land plants. New Phytol 154:275–304CrossRefGoogle Scholar
  9. Burke DJ, Zhu S, Pablico-Lansigan MP, Hewins CR, Samia ACS (2014) Titanium oxide nanoparticle effects on composition of soil microbial communities and plant performance. Biol Fertil Soils 50:1169–1173CrossRefGoogle Scholar
  10. Cameron DD, Neal AL, van Wees SCM, Ton J (2013) Mycorrhiza-induced resistance: more than the sum of its parts? Trends Plant Sci 18:539–545CrossRefPubMedPubMedCentralGoogle Scholar
  11. Cao J, Feng Y, Lin X, Wang J (2016) Arbuscular mycorrhizal fungi alleviate the negative effects of iron oxide nanoparticles on bacterial community in rhizospheric soils. Front Environ Sci 4:1–10. doi: 10.3389/fenvs.2016.00010 CrossRefGoogle Scholar
  12. Caron M, Fortin JA, Richard C (1985) Effect of Glomus intraradices on infection by Fusarium oxysporum f. sp. radicicopersici on tomato over a 12 week period. Can J Bot 64:552–556CrossRefGoogle Scholar
  13. Chalot M, Blaudez D, Brun A (2006) Ammonia: a candidate for nitrogen transfer at the mycorrhizal interface. Trends Plant Sci 11:263–266CrossRefPubMedGoogle Scholar
  14. de la Rosa G, Lopez-Moreno ML, de Haro D, Botez CE, Peralta-Videa JR, Gardea-Torresdey JL (2013) Effects of ZnO nanoparticles in alfalfa, tomato, and cucumber at the germination stage: root development and X-ray absorption spectroscopy studies. Pure Appl Chem 85:2161–2174Google Scholar
  15. Dubchak S, Ogar A, Mietelski JW, Turnau K (2010) Influence of silver and titanium nanoparticles on arbuscular mycorrhiza colonization and accumulation of radio-caesium in Helianthus annuus. Spanish J Agric Res 8:103–108CrossRefGoogle Scholar
  16. Feng Y, Cui X, He S, Dong G, Chen M, Wang J, Lin X (2013) The role of metal nanoparticles in influencing arbuscular mycorrhizal fungi effects on plant growth. Environ Sci Technol 47:9496–9504CrossRefPubMedGoogle Scholar
  17. Gajanan G, Deuk SY, Donghee P, Sung LD (2010) Phytotoxicity of carbon nanotubes assessed by Brassica Juncea and Phaseolus Mungo. J Nanoelectron Optoelectron 5:157–160CrossRefGoogle Scholar
  18. George TS, Dou D, Wang X (2016) Plant-microbe interactions: manipulating signals to enhance agricultural sustainability and environmental security. Plant Growth Regul 80:1–3CrossRefGoogle Scholar
  19. Gopinath K, Gowri S, Karthika V, Arumugam A (2014) Green synthesis of gold nanoparticles from fruit extract of Terminalia arjuna, for the enhanced seed germination activity of Gloriosa superba. J Nanostruct Chem 4:1–11Google Scholar
  20. Gruyer N, Dorais M, Bastien C, Dassylva N, Triffault-Bouchet G (2013) Interaction between sliver nanoparticles and plant growth. International symposium on new technologies for environment control, energy-saving and crop production in greenhouse and plant factory–greensys, Jeju, Korea, 6–11 Oct 2013Google Scholar
  21. Harrier LA, Watson CA (2004) The potential role of arbuscular mycorrhizal (AM) fungi in the bioprotection of plants against soil-borne pathogens in organic and/or other sustainable farming systems. Pest Manag Sci 60:149–157CrossRefPubMedGoogle Scholar
  22. Husen A, Siddiqi KS (2014) Carbon and fullerene nanomaterials in plant system. J Nanotechnol 12:1–10Google Scholar
  23. Jaberzadeh A, Moaveni P, Moghadam HRT, Zahedi H (2013) Influence of bulk and nanoparticles titanium foliar application on some agronomic traits, seed gluten and starch contents of wheat subjected to water deficit stress. Not Bot Hortic Agrobot 41:201–207Google Scholar
  24. Kaur R, Singh A, Kang JS (2014) Influence of different types mycorrhizal fungi on crop productivity. Curr Agric Res J 2:51–54CrossRefGoogle Scholar
  25. Krishnaraj C, Jagan EG, Ramachandran R, Abirami SM, Mohan N, Kalaichelvan PT (2012) Effect of biologically synthesized silver nanoparticles on Bacopa monnieri (Linn.) Wettst. Plant growth metabolism. Process Biochem 47:51–58CrossRefGoogle Scholar
  26. Li S, Liu XQ, Wang FY, Miao YF (2015) Effects of ZnO nanoparticles, ZnSO4 and arbuscular mycorrhizal fungus on the growth of maize. Huan Jing KeXue 36:4615–4622Google Scholar
  27. Lin D, Xing B (2007) Phytotoxicity of nanoparticles: inhibition of seed germination and root growth. Environ Pollut 150:243–250CrossRefPubMedGoogle Scholar
  28. Mahajan P, Dhoke SK, Khanna AS (2011) Effect of nano-ZnO particle suspension on growth of mung (Vigna radiata) and gram (Cicer arietinum) seedlings using plant agar method. J Nanotechnol:1–7. doi: 10.1155/2011/696535
  29. Mahmoodzadeh H, Nabavi M, Kashefi H (2013) Effect of nanoscale titanium dioxide particles on the germination and growth of canola (Brassica napus). J Ornamental Hortic Plants 3:25–32Google Scholar
  30. Manjunatha SB, Biradar DP, Aladakatti YR (2016) Nanotechnology and its applications in agriculture: a review. J Farm Sci 29:1–13Google Scholar
  31. Mondal A, Basu R, Das S, Nandy P (2011) Beneficial role of carbon nanotubes on mustard plant growth: an agricultural prospect. J Nanopart Res 13:4519–4528CrossRefGoogle Scholar
  32. Morla S, Ramachandra Rao CSV, Chakrapani R (2011) Factors affecting seed germination and seedling growth of tomato plants cultured in vitro conditions. J Chem Bio Phys Sci B 1:328–334Google Scholar
  33. Nalwade AR, Neharkar SB (2013) Carbon nanotubes enhance the growth and yield of hybrid Bt cotton Var. ACH-177-2. Int J Adv Sci Tech Res 3:840–846Google Scholar
  34. Naqvi NS, Naqvi SAMH (2007) Mycorrhiza in management of fruits and vegetables diseases. Dis Fruits Veg 2:537–558Google Scholar
  35. Prasad K (1998) Biological control of rhizospheric microflora of Saccharum officinarum L. plants through vesicular arbuscular mycorrhizal (Glomus fasciculatum) fungi. Biome 8:131–136Google Scholar
  36. Prasad TNVKV, Sudhakar P, Sreenivasulu Y, Latha P, Munaswamy V, Reddy KR, Sreeprasad TSP, Sajanlal R, Pradeep T (2012) Effect of nanoscale zinc oxide particles on the germination, growth and yield of peanut. J Plant Nutr 35:905–927CrossRefGoogle Scholar
  37. Prasad R, Bhola D, Akdi K, Cruz C, Sairam KVSS, Tuteja N, Varma A (2017a) Introduction to mycorrhiza: historical development. In: Varma A, Prasad R, Tuteja N (eds) Mycorrhiza. Springer, Cham, pp 1–7Google Scholar
  38. Prasad R, Bhattacharyya A, Nguyen QD (2017b) Nanotechnology in sustainable agriculture: recent developments, challenges, and perspectives. Front Microbiol 8:1014. doi: 10.3389/fmicb.2017.01014
  39. Rai M, Ingle A (2012) Role of nanotechnology in agriculture with special reference to management of insect pests. Appl Microbiol Biotechnol 94:287–293CrossRefPubMedGoogle Scholar
  40. Raliya R, Tarafdar JC (2013) ZnO nanoparticle biosynthesis and its effect on phosphorous-mobilizing enzyme secretion and gum contents in cluster bean (Cyamopsis tetragonoloba L.) Agric Res 2:48–57CrossRefGoogle Scholar
  41. Ramesh M, Palanisamy K, Babu K, Sharma NK (2014) Effects of bulk & nano-titanium dioxide and zinc oxide on physio-morphological changes in Triticum aestivum Linn. J Glob Biosci 3:415–422Google Scholar
  42. Raskar SV, Laware SL (2014) Effect of zinc oxide nanoparticles on cytology and seed germination in onion. Int J Curr Microbiol Appl Sci 3:467–473Google Scholar
  43. Rathod DP, Brestic M, Shao HB (2011) Chlorophyll a fluorescence determines the drought resistance capabilities in two varieties of mycorrhized and non-mycorrhized Glycine max Linn. Afr J Microbiol Res 5:4197–4206CrossRefGoogle Scholar
  44. Razzaq A, Ammara R, Jhanzab HM, Mahmood T, Hafeez A, Hussain S (2016) A novel nanomaterial to enhance growth and yield of wheat. J Nanosci Technol 2:55–58Google Scholar
  45. Savithramma N, Ankanna S, Bhumi G (2012) Effect of nanoparticles on seed germination and seedling growth of Boswellia ovalifoliolata an endemic and endangered medicinal tree taxon. Nano Vision 2:61–68Google Scholar
  46. Schouteden N, Waele DD, Panis B, Vos CM (2015) Arbuscular mycorrhizal fungi for the biocontrol of plant-parasitic nematodes: a review of the mechanisms involved. Front Microbiol 6:1280. doi: 10.3389/fmicb.2015.01280 CrossRefPubMedPubMedCentralGoogle Scholar
  47. Schüßler A, Schwarzott D, Walker C (2001) A new fungal phylum, the Glomeromycota: phylogeny and evolution. Mycol Res 105:1413–1421CrossRefGoogle Scholar
  48. Sedghi M, Hadi M, Toluie SG (2013) Effect of nano zinc oxide on the germination of soybean seeds under drought stress. Ann West Univ Timisoara Ser Biol XVI 2:73–78Google Scholar
  49. Seeger EM, Baun A, Kanstner M, Trapp S (2009) Insignificant acute toxicity of TiO2 nanoparticles to willow trees. J Soils Sediments 9:46–53CrossRefGoogle Scholar
  50. Shah V, Belozerova I (2009) Influence of metal nanoparticles on the soil microbial community and germination of lettuce seeds. Water Air Soil Pollut 197:143–148CrossRefGoogle Scholar
  51. Sharma P, Bhatt D, Zaidi MG, Saradhi PP, Khanna PK, Arora S (2012) Silver nanoparticle mediated enhancement in growth and antioxidant status of Brassica juncea. Appl Biochem Biotechnol 167:2225–2233CrossRefPubMedGoogle Scholar
  52. Siddiqui MH, Al-Whaibi MH (2014) Role of nano-SiO2 in germination of tomato (Lycopersicum esculentum seeds Mill.) Saudi Biol Sci 21:13–17CrossRefGoogle Scholar
  53. Siddiqui MH, Al-Whaibi MH, Firoz M, Al-Khaishany MY (2015) Role of nanoparticles in plants. In: Siddiqui MH, Al-Whaibi MH, Firoz M (eds) Nanotechnology and plant sciences. Springer, Cham, pp 19–35Google Scholar
  54. Smith SE, Smith FA (2011) Roles of arbuscular mycorrhizas in plant nutrition and growth: new paradigms from cellular to ecosystem scales. Ann Rev Plant Biol 62:227–250CrossRefGoogle Scholar
  55. Suman, Prasad R, Jain VK, Varma A (2010) Role of nanomaterials in symbiotic fungus growth enhancement. Curr Sci 99:1189–1191Google Scholar
  56. Suriyaprabha R, Karunakaran G, Yuvakkumar R, Rajendran V, Kannan N (2012) Silica nanoparticles for increased silica availability in maize (Zea mays L) seeds under hydroponic conditions. Curr Nanosci 8:902–908CrossRefGoogle Scholar
  57. Sweet MJ, Singleton I (2015) Soil contamination with silver nanoparticles reduces Bishop pine growth and ectomycorrhizal diversity on pine roots. J Nanopart Res 17:448. doi: 10.1007/s11051-015-3246-4 CrossRefPubMedPubMedCentralGoogle Scholar
  58. Syu YY, Hung JH, Chen JC, Chuang HW (2014) Impacts of size and shape of silver nanoparticles on Arabidopsis plant growth and gene expression. Plant Physiol Biochem 83:57–64CrossRefPubMedGoogle Scholar
  59. Tawaraya K (2003) Arbuscular mycorrhizal dependency of different plant species and cultivars. Soil Sci Plant Nutr 49:655–668CrossRefGoogle Scholar
  60. Tripathi DK, Shweta SS, Swati S, Pandey R, Singh VP, Prasad SM, Dubey NK, Chauhan DK (2017) An overview on manufactured nanoparticles in plants: uptake, translocation, accumulation and phytotoxicity. Plant Physiol Biochem 110:2–12CrossRefPubMedGoogle Scholar
  61. Wang F, Liu X, Shi Z, Tong R, Adams CA, Shi X (2016) Arbuscular mycorrhizae alleviate negative effects of zinc oxide nanoparticle and zinc accumulation in maize plants – a soil microcosm experiment. Chemosphere 147:88–97CrossRefPubMedGoogle Scholar
  62. Yin L, Colman BP, McGill BM, Wright JP, Bernhardt ES (2012) Effects of silver nanoparticle exposure on germination and early growth of eleven wetland plants. PLoS ONE 7:1–7CrossRefGoogle Scholar

Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  • Avinash Ingle
    • 1
  • Dnyaneshwar Rathod
    • 1
  • Ajit Varma
    • 2
  • Mahendra Rai
    • 1
    Email author
  1. 1.Nanobiotechnology Laboratory, Department of BiotechnologySGB Amravati UniversityAmravatiIndia
  2. 2.Amity Institute of Microbial TechnologyAmity UniversityNoidaIndia

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