Abstract
Red blotch is a recently recognized disease of grapevine for which the graft-transmissible grapevine red blotch-associated virus (GRBaV), a proposed member of a new genus within the family Geminiviridae, is the causal agent. The virus affects fruit quality, delays ripening, and probably reduces yield and vigor. Estimated economic losses range from $2,213 to $68,548 per hectare over a 25-year productive life span of a vineyard. The genome of GRBaV is circular and consists of a single molecule of single-stranded DNA with seven predicted open reading frames. Foliar symptoms consist of red blotches that expand and coalesce in late summer and fall and irregular chlorotic areas that become necrotic later in the season in red-berried and white-berried Vitis vinifera cultivars, respectively. Visual diagnosis is often unreliable due to several confounding abiotic and biotic factors, including similarities with leafroll disease symptoms; therefore, PCR-based assays are recommended for an accurate diagnosis. Although red blotch disease was only recognized in 2008, GRBaV was detected in archival grapevine leaves sampled in 1940 in California and kept in a herbarium collection, suggesting the virus was present in vineyards more than 70 years prior to its identification. Surveys of vineyards revealed the occurrence of GRBaV in some of the major grape-growing regions in the USA and Canada. Outside of North America, the virus was found so far in Switzerland in material introduced from the USA and in South Korea. An early account of the Virginia creeper leafhopper as a vector of GRBaV was not confirmed; instead, the three-cornered alfalfa treehopper was shown to be a likely vector of epidemiological significance. Disease management strategies almost exclusively rely on roguing and replacing vineyards using planting material derived from clean, virus-tested stocks. Advancing our understanding of disease epidemiology and viral gene expression are important future research topics for red blotch disease and GRBaV.
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References
Al Rwahnih, M., D. Ashita, M. Anderson, A. Rowhani, J.K. Uyemoto, and M.R. Sudarshana. 2013. Association of a DNA virus with grapevines affected by red blotch disease in California. Phytopathology 103: 1069–1076.
Al Rwahnih, M., A. Rowhani, D. Golino, C. Islas, J. Preece, and M.R. Sudarshana. 2015a. Detection and genetic diversity of Grapevine red blotch-associated virus isolates in table grape accessions in the National Clonal Germplasm Repository in California. Canadian Journal of Plant Pathology 37: 130–135.
Al Rwahnih, M., A. Rowhani, and D. Golino. 2015b. First report of grapevine red blotch-associated virus in archival grapevine material from Sonoma County, California. Plant Disease 99: 895.
Atallah, S., M. Gomez, M. Fuchs, and T. Martinson. 2012. Economic impact of grapevine leafroll disease on Vitis vinifera cv. Cabernet franc in Finger Lakes vineyards of New York. American Journal of Enology and Viticulture 63: 73–79.
Bahder, B.W., F.G. Zalom, and M.R. Sudarshana. 2016a. An evaluation of the flora adjacent to wine grape vineyards for the presence of alternative host plants of Grapevine red blotch-associated virus. Plant Disease 100: 1571–1574.
Bahder, B., F. Zalom, M. Jayanth, and M. Sudarshana. 2016b. Phylogeny of geminivirus coat protein sequences and digital PCR aid in identifying Spissistilus festinus (Say) as a vector of Grapevine red blotch-associated virus. Phytopathology 106: 1223–1230.
Calvi, B.L. 2011. Effects of red-leaf disease on Cabernet Sauvignon at the Oakville experimental vineyard and mitigation by harvest delay and crop adjustment. M.S. thesis, University of California, Davis, CA.
Fuchs, M., B. Krenz, L.M. Yepes, J. Thompson, H. McLane, and K.L. Perry. 2015. Is Grapevine red blotch-associated virus the causal agent of red blotch disease? In: Proceedings of the 18th Congress of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine, 72–73.
Guo, T.W., D. Vimalesvaran, J.R. Thompson, K.L. Perry, and B. Krenz. 2015. Subcellular localization of grapevine red blotch-associated virus ORFs V2 and V3. Virus Genes 51: 156–158.
Krenz, B., J. Thompson, M. Fuchs, and K.L. Perry. 2012. Complete genome sequence of a new circular DNA virus from grapevine. Journal of Virology 86: 7715.
Krenz, B., J.R. Thompson, H. McLane, M. Fuchs, and K.L. Perry. 2014. Grapevine red blotch-associated virus is widespread in the United States. Phytopathology 102: 1232–1240.
Lim, S., D. Igori, F. Zhao, and J.S. Moon. 2016. First report of Grapevine red blotch-associated virus on grapevine in Korea. Plant Disease 100: 1957.
Mitchell, P., and L. Newsom. 1984. Seasonal history of the three cornered alfalfa hopper (Homoptera: Membracidae) in Louisiana. Journal of Economic Entomology 77: 906–914.
Perry, K.L., H. McLane, M.Z. Hyder, G.S. Dangl, J.R. Thompson, and M.F. Fuchs. 2016. Grapevine red blotch-associated virus is present in free-living Vitis sp. proximal to cultivated grapevines. Phytopathology 106: 663–670.
Piepenburg, O., C.H. Williams, D.L. Stemple, and N.A. Armes. 2006. DNA detection using recombination proteins. PLoS Biology 4: 1115–1121.
Poojari, S., O.J. Alabi, V.Y. Fofanov, and R.A. Naidu. 2013. A leafhopper-transmissible DNA virus with novel evolutionary lineage in the family geminiviridae implicated in grapevine redleaf disease by next-generation sequencing. PloS One 8: e64194.
Poojari, S., T. Lowery, A-M Schmidt, M. Rott, W. Mcfadden-Smith, L. Stobbs, and J.R. Urbez-Torres. 2016. Red blotch and the virus in Canada. In: Webinar on red blotch disease, 26 February, http://www.ipmcenters.org/index.cfm/center-products/ipm-eacademy/upcoming-events/red-blotch-speakers/
Reynard, J.S. 2015. Survey of emerging viruses in Switzerland. In: Proceedings of the 18th Congress of the International Council for the Study of Virus and Virus-like Diseases of the Grapevine, 223-224.
———. 2016. Red blotch and the virus in Europe. IPM eAcademy webinar. 26 February 2016. https://www.youtube.com/watch?v=LEGXd3iLBx4&list=PLK93eCG1nCqKFZWFWuMqKPyAvU-Qk--B3&index=7
Ricketts, K.D., M.I. Gomez, S.S. Atallah, M.F. Fuchs, T. Martinson, R.J. Smith, P.S. Verdegaal, M.L. Cooper, L.J. Bettiga, and M.C. Battany. 2015. Reducing the economic impact of grapevine leafroll disease in California: Identifying optimal management practices. American Journal of Enology and Viticulture 66: 138–147.
Ricketts, K.D., M.I. Gomez, M.F. Fuchs, T.E. Martinson, R.J. Smith, M.L. Cooper, M. Moyer, and A. Wise. 2016. Mitigating the economic impact of grapevine red blotch: Optimizing disease management strategies in U.S. Vineyards. American Journal of Enology and Viticulture 68: 127–135.
Seguin, J., R. Rajeswaran, N. Malpica-Lopez, R.R. Martin, K. Kasschau, V.V. Dolja, P. Otten, L. Farinelli, and M.M. Poogin. 2014. De novo reconstruction of consensus master genomes of plant RNA and DNA viruses from siRNAs. PloS One 9: e88513.
Sudarshana, M.R., K.L. Perry, and M.F. Fuchs. 2015. Grapevine red blotch-associated virus, an emerging threat to the grapevine industry. Phytopathology 105: 1026–1032.
Varsani, A., J. Navas-Castillo, E. Moriones, C. Hernández-Zepeda, A. Idris, J.K. Brown, F. Murilo Zerbini, and D.P. Martin. 2014. Establishment of three new genera in the family Geminiviridae: Becurtovirus, Eragrovirus and Turncurtovirus. Archives of Virology 159: 2193–2203.
Acknowledgments
The authors acknowledge the valued conversations and contributions of lab members and numerous cooperators over the years of study. Thanks to the American Vineyard Foundation, the New York Grape and Wine Foundation, USDA-NIFA-Agriculture Food and Research Initiative-Critical Agriculture Research and Extension, USDA-APHIS-National Clean Plant Network, USDA-NIFA-Capacity Grant, Kaplan and Saltonstall Endowment Funds, and Nolan fellowship funds for their support.
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Cieniewicz, E., Perry, K., Fuchs, M. (2017). Grapevine Red Blotch: Molecular Biology of the Virus and Management of the Disease. In: Meng, B., Martelli, G., Golino, D., Fuchs, M. (eds) Grapevine Viruses: Molecular Biology, Diagnostics and Management. Springer, Cham. https://doi.org/10.1007/978-3-319-57706-7_14
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DOI: https://doi.org/10.1007/978-3-319-57706-7_14
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