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Expression of Epithelial Mesenchymal Transition and Cancer Stem Cell Markers in Circulating Tumor Cells

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Isolation and Molecular Characterization of Circulating Tumor Cells

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 994))

Abstract

The characterization of circulating tumor cells (CTC) has the potential not only to provide important insights into molecular alterations of advanced tumor disease but also to facilitate risk prediction. Epithelial mesenchymal transition (EMT) has been discovered as important process for the development of metastases and the dissemination of tumor cells into the blood stream. In different tumor types, CTC with a mesenchymal phenotype have been reported that have presumably underwent EMT. Moreover, CTC with stem-cell like characteristics have been postulated as important drivers of tumor progression. Different platforms have been introduced to allow CTC enrichment independent of expression of epithelial antigens, as these may be downregulated in EMT- or stem-cell-like CTC. Both for CTCs with EMT- or stem-cell features different markers have been proposed. However, there is still a lack of evidence on the association of these markers with functional features and characteristics for stem cells and cells undergoing EMT.

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Authors and Affiliations

  1. Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany

    Stefan Werner & Klaus Pantel

  2. Department of Urology, Eberhard-Karls-University, Teubingen, Germany

    Arnulf Stenzl & Tilman Todenhöfer

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  1. Stefan Werner
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  2. Arnulf Stenzl
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Correspondence to Tilman Todenhöfer .

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  1. University of California San Francisco, San Francisco, California, USA

    Mark Jesus M. Magbanua

  2. University of California San Francisco, San Francisco, California, USA

    John W. Park

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Werner, S., Stenzl, A., Pantel, K., Todenhöfer, T. (2017). Expression of Epithelial Mesenchymal Transition and Cancer Stem Cell Markers in Circulating Tumor Cells. In: Magbanua, M., Park, J. (eds) Isolation and Molecular Characterization of Circulating Tumor Cells. Advances in Experimental Medicine and Biology, vol 994. Springer, Cham. https://doi.org/10.1007/978-3-319-55947-6_11

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