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Optimization of Cyclic Voltammetric Curve Parameters to Measure Lactate Concentration in Urine Samples

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Sensors (CNS 2016)

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 431))

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Abstract

In this work, veal urine dilutions in Hepes-buffered Ringer’s solution (HBRS) are tested by both UV-visible absorption spectroscopy and Cyclic Voltammetry (CV) to assess their viability as mediums for the detection of lactate, through the Lactate Dehydrogenase enzyme (LDH) reaction which involves the formation of NADH. Several data analysis algorithms for the recorded CV data are proposed and compared, in order to optimize the NADH detection in the urine samples dilutions. UV-visible spectroscopy was adopted as reference for NADH quantification.

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Acknowledgements

The research activities reported here have been partially supported by the project «Sistema microelettronico per l’individuazione tempestiva di trattamenti illeciti sugli animali da allevamento», co-founded by Avepa (Veneto Region): Programma di sviluppo rurale, Misura 124 —Cooperazione per lo sviluppo di nuovi prodotti, processi e tecnologie nel settore agricolo, alimentare e forestale. We particularly thank UNICARVE for their contribution to the project activities.

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Authors and Affiliations

  1. Department of Information Engineering, University of Padova, via G. Gradenigo 6/b, 35131, Padua, Italy

    Giulio Rosati, Elisabetta Pasqualotto & Alessandro Paccagnella

  2. ARC - Centro Ricerche Applicate s.r.l, via J. da Montagnana, 49, 35132, Padua, Italy

    Matteo Scaramuzza, Elisabetta Pasqualotto & Alessandro De Toni

Authors
  1. Giulio Rosati
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  2. Matteo Scaramuzza
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  3. Elisabetta Pasqualotto
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  4. Alessandro De Toni
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  5. Alessandro Paccagnella
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Corresponding author

Correspondence to Giulio Rosati .

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Editors and Affiliations

  1. University of Catania, Catania, Italy

    Bruno Andò

  2. IFAC-CNR, Sesto Fiorentino, Italy

    Francesco Baldini

  3. University of Rome Tor Vergata, Rome, Italy

    Corrado Di Natale

  4. University of Florence, Sesto Fiorentino, Italy

    Giovanna Marrazza

  5. IMM-CNR, Lecce, Italy

    Pietro Siciliano

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Rosati, G., Scaramuzza, M., Pasqualotto, E., De Toni, A., Paccagnella, A. (2018). Optimization of Cyclic Voltammetric Curve Parameters to Measure Lactate Concentration in Urine Samples. In: Andò, B., Baldini, F., Di Natale, C., Marrazza, G., Siciliano, P. (eds) Sensors. CNS 2016. Lecture Notes in Electrical Engineering, vol 431. Springer, Cham. https://doi.org/10.1007/978-3-319-55077-0_14

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  • DOI: https://doi.org/10.1007/978-3-319-55077-0_14

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  • Print ISBN: 978-3-319-55076-3

  • Online ISBN: 978-3-319-55077-0

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