Abstract
As mentioned in Chap. 3, while immunoassays have an extensive application in both clinic and research, the immunosensor concept is yet to be implemented in routine diagnostics [23, 24]. There are certain issues in this regard, the most important of which is the coupling chemistry of proteins on surfaces, which is critical for optimal functioning. In other words, the optimal orientation of Ab molecules on the surface is of great importance in selective and sensitive detection in a reproducible way without affecting its activity [25]. The relative lack of long-term stability of biological molecules is the most important limitation in commercializing biosensors.
Therefore, this chapter deals with the immobilization of two main BTMs (Oc and CTX) on the glass-based gold electrode fabricated as mentioned in Chap. 5 and coated with AuNPs. To our knowledge, a biosensor to assess serum levels of Oc has not been developed before. A stepwise protocol to develop these sensors is explained here.
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Khashayar, P. (2017). Oc and CTX BioSensors: Characteristics and Validation. In: Point-of-Care Solution for Osteoporosis Management. Springer, Cham. https://doi.org/10.1007/978-3-319-55053-4_6
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