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Micropropagation of Cannabis sativa L.—An Update

Abstract

Cannabis is one of the oldest economically important plant yielding fiber, food and medicine. It is a natural source of Δ9-tetrahydrocannabinol (THC) and Cannabidiol (CBD). These two molecules have a tremendous therapeutic potential and commercial value in the pharmaceutical area. Cannabis is a highly heterozygous species. Being dioceous (male and female flowers appear on two different plants) and wind pollinated species, it is difficult to maintain the chemical profile of biomass product, if grown from seed. Plant to plant variation is observed even though plants are grown from seeds obtained from a single female plant. Therefore, to maintain consistency in the end product, elite female plants are screened and multiplied using vegetative propagation and/or tissue culture. Micro propagation can play a vital role in the conservation of elite Cannabis clones and rapid multiplication of novel germplasm. On the other hand, it can also be used in genetic modification for the enhanced cannabinoid production. Research on in vitro propagation of Cannabis has resulted in the development of protocols for callus production, cell suspension cultures, agrobacterium mediated hairy root cultures and regeneration of plants. This chapter provides an overview of in vitro propagation of Cannabis and addresses the current applications of modern biotechnology in propagation of elite Cannabis plants.

Keywords

  • Shoot Regeneration
  • Hairy Root Culture
  • Nodal Segment
  • Female Plant
  • Callus Production

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Acknowledgements

This work was supported in part by the National Institute on Drug Abuse (NIDA), National Institute of Health (NIH), Department of Health and Human Services, USA, Contract No. N01DA-15-7793.

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Correspondence to Mahmoud A. ElSohly .

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Lata, H., Chandra, S., Khan, I.A., ElSohly, M.A. (2017). Micropropagation of Cannabis sativa L.—An Update. In: Chandra, S., Lata, H., ElSohly, M. (eds) Cannabis sativa L. - Botany and Biotechnology. Springer, Cham. https://doi.org/10.1007/978-3-319-54564-6_13

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