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Enhanced Bacteriophages

  • Sabah A. A. JassimEmail author
  • Richard G. Limoges
Chapter

Abstract

The bacterial cell wall is the most important part of the bacterial structure for bacteriophage attachment, which is required to initiate infection. The rapid and precise attachment of the phage onto a susceptible host cell is the first step of infection. In this chapter, methods are described to control phage-host interactions and to produce highly lytic phages with no or far less phage-resistant mutants, along with broad host targeting capabilities. These methods do not employ genetic modification to breed ‘re-tailored’ wild phages with auxiliary mechanisms for phage adherence, adsorption, binding and uptake which are critical for plaque formation. The purpose of these tactics is to gain new sub-strains of phages that are able to infect previously resistant bacteria and to play an important role in future applications.

Keywords

Bacteriophage Phage design Phage breeding Phage reprogramming technology 

References

  1. Abdulamir AS, Jassim SAA, Abu Bakar F (2014) Novel approach of using a cocktail of designed bacteriophages against gut pathogenic E. coli for bacterial load biocontrol. Ann Clin Microbiol Antimicrob 13:39. http://www.ann-clinmicrob.com/content/pdf/s12941-014-0039-z.pdf
  2. Aldoori AA, Mahdii EF, Abbas AK, Jassim SAA (2015) Bacteriophage biocontrol rescues mice bacteremic of clinically isolated mastitis from dairy cows associated with methicillin-resistant Staphyloccocus aureus. Adv Microbiol 5:383–403. http://www.scirp.org/journal/PaperInformation.aspx?PaperID=56931
  3. Ausubel FM, Brent R, Kingstone RE et al (1991) Growing lambda-derived vectors. In: Asdf A (ed) Current protocols in molecular biology, vol 1. Wiley Interscience, New York, pp 12.1–12.3Google Scholar
  4. Hibma AM, Jassim SAA, Griffiths MW (1997) Infection and removal of L-forms of Listeria monocytogenes with bred bacteriophage. Int J Food Microbiol 34:197–207CrossRefPubMedGoogle Scholar
  5. Jassim SAA, Limoges RG (2013) Impact of external forces on cyanophage—host interactions in aquatic ecosystems. World J Microbiol Biotechnol 29:1751–1762CrossRefPubMedGoogle Scholar
  6. Jassim SAA, Limoges RG (2014) Natural solution to antibiotic resistance: bacteriophages ‘The Living Drugs’. World J Microbiol Biotechnol 30:2153–2170CrossRefPubMedPubMedCentralGoogle Scholar
  7. Jassim SAA, Denyer SP, Stewart GSAB (1995) Selective virus culture. WO/1995/023848. http://patentscope.wipo.int/search/en/WO1995023848
  8. Jassim SAA, Abdulamir AS, Abu Bakar F (2010) Methods for bacteriophage design. WO/2010/064044. http://www.wipo.int/pctdb/en/wo.jsp?WO=2010064044
  9. Jassim SAA, Abdulamir AS, Abu Bakar F (2012) Novel phage-based bio-processing of pathogenic Escherichia coli and its biofilms. World J Microbiol Biotechnol 28:47–60CrossRefPubMedGoogle Scholar
  10. Maxwell KL (2016) Phages fight back: inactivation of the CRISPR-Cas bacterial immune system by anti-CRISPR proteins. PLoS Pathog 12(1):e1005282. doi: 10.1371/journal.ppat.1005282 CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  1. 1.Applied Bio Research Inc.WindsorCanada

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