The bacterial cell wall is the most important part of the bacterial structure for bacteriophage attachment, which is required to initiate infection. The rapid and precise attachment of the phage onto a susceptible host cell is the first step of infection. In this chapter, methods are described to control phage-host interactions and to produce highly lytic phages with no or far less phage-resistant mutants, along with broad host targeting capabilities. These methods do not employ genetic modification to breed ‘re-tailored’ wild phages with auxiliary mechanisms for phage adherence, adsorption, binding and uptake which are critical for plaque formation. The purpose of these tactics is to gain new sub-strains of phages that are able to infect previously resistant bacteria and to play an important role in future applications.
KeywordsBacteriophage Phage design Phage breeding Phage reprogramming technology
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