Medicinal Plants: Ethno-Uses to Biotechnology Era



To date, medicinal plants form the backbone of primary healthcare for 70–95% of the population of the developing world. Therefore, medicinal plants help in alleviating human suffering and are widely used for traditional remedies, pharmaceutical materials, and trade. Cancer patients numbers are increasing worldwide, ranking this disease as the second disease cause of mortality for both sexes. Traditionally, medicinal plants have been used in the fight against cancer, then it is considered as the basis for medicines discovery, and nowadays more than 70% of anticancer drugs have a natural source. The biotechnological tools are necessary to select, multiply, improve, and analyze medicinal plants. This chapter highlights the history of using the medicinal plants indigenously worldwide, i.e., anticancer reservoirs and also answers to many questions, such as: Why the importance of using medicinal plants is increasing recently? What are the benefits of applying the biotechnology in medicinal plants? It then describes the new biotech technique of the traceability by using PCR-DGGE to determine the geographical origin of medicinal plants (a case study of Physalis fruits from four different countries) by analyzing the DNA fragments of microorganisms (yeasts) on plants. This method is based on the assumption that the microbial communities of environmental samples are unique to a geographic area.


Ethnomedicine history Medicinal plants Anticancer reservoirs Benefits of biotechnology Traceability PCR-DGGE Origin 


  1. Abera B (2014) Medicinal plants used in traditional medicine by Oromo people, Ghimbi district, Southwest Ethiopia. J Ethnobiol Ethnomed 10:40. Accessed 16 July 2016
  2. Abramov V (1996) Traditional medicine, vol 134. World Health Organization, Geneva, pp 1–3Google Scholar
  3. Adefa M, Abraha B (2011) Ethnobotanical survey of traditional medicinal plants in Tehuledere district, South Wollo, Ethiopia. J Med Plant Res 5(26):6233–6242Google Scholar
  4. AESGP (Association Européenne des Spécialités Pharmaceutiques Grand Public; The Association of the European Self-Medication Industry) (1998) Herbal medicinal products in the European Union. Study carried out on behalf of the European Commission, Brussels. Accessed 16 July 2016
  5. Aggarwal BB, Sundaram C, Malani N, Ichikawa H (2007) Curcumin: the Indian solid gold. In: Aggarwal BB, Surh Y-J, Shishodia S (eds) The molecular targets and therapeutic uses of curcumin in health and disease. Springer, New York, NYCrossRefGoogle Scholar
  6. Agoreyo BO, Okoro NC, Choudhary MI (2012) Preliminary phytochemical analysis of two varieties of Adenia lobata (Jacq) and the antioxidant activity of their various solvent fractions. Bayero J Pure Appl Sci 5(1):182–186. doi: 10.4314/bajopas.v5i1.32 Google Scholar
  7. Ahmed E, Arshad M, Saboor A, Qureshi R, Mustafa G, Sadiq S, Chaudary KS (2013) Ethnobotanical appraisal and medicinal use of plants in Patriata, New Murree, evidence from Pakistan. J Ethnobiol Ethnomed 9:13. Accessed 16 July 2016
  8. AHPA-ERB (AHPA Foundation for Education & Research on Botanicals) (2008) A field guide to herbal dietary supplements. AHPA-ERB Foundation, Silver Spring, MD. Accessed 16 July 2016
  9. Al-Harbi MM, Qureshi S, Ahmed MM, Raza M, Baig MZA, Shah AH (1996) Effect of camel urine on the cytological and biochemical changes induced by cyclophosphamide in mice. J Ethnopharmacol 52(3):129–137PubMedCrossRefGoogle Scholar
  10. Ali NA, Julich WD, Kusnick C, Lindequist U (2001) Screening of Yemeni medicinal plants for antibacterial and cytotoxic activities. J Ethnopharmacol 74:173–179PubMedCrossRefGoogle Scholar
  11. Ali-Shtayeh MS, Yaniv Z, Mahajna J (2000) Ethnobotanical survey in the Palestinian area: a classification of the healing potential of medicinal plants. J Ethnopharmacol 73:221–232PubMedCrossRefGoogle Scholar
  12. Almeida CFCBR, Amorim ELC, Albuquerque UP, Maia MBS (2006) Medicinal plants popularly used in the Xingó region – a semi-arid location in Northeastern Brazil. J Ethnobiol Ethnomed 2:1–7CrossRefGoogle Scholar
  13. Al-Quraʼn S (2009) Ethnopharmacological survey of wild medicinal plants in Showbak, Jordan. J Ethnopharmacol 123:45–50PubMedCrossRefGoogle Scholar
  14. Al-Sokari SS, El Sheikha AF (2015) In vitro antimicrobial activity of crude extracts of some medicinal plants from Al-Baha region in Saudi Arabia. J Food Nutr Sci 3(1–2):74–78Google Scholar
  15. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein databases search programs. Nucleic Acids Res 25(17):3389–3402PubMedPubMedCentralCrossRefGoogle Scholar
  16. Arias TD (1999) Glosario de Medicamentos: desarollo, evaluaciõn y uso. Organizaciõn Panamricana de La Salud. Organizaciõn Mundial de La Salud, Washington, DCGoogle Scholar
  17. Arsdall AV (2002) Medieval herbal remedies: the old English herbarium and Anglo-Saxon Medicine. Psychology Press, New York, NYGoogle Scholar
  18. Azaizeh H, Saad B, Cooper E, Said O (2010) Traditional Arabic and Islamic medicine, a re-emerging health aid. Evid Based Complement Alternat Med 7(4):419–424PubMedCrossRefGoogle Scholar
  19. Baek SH, Bae ON, Park JH (2012) Recent methodology in ginseng analysis. J Ginseng Res 36:119–134PubMedPubMedCentralCrossRefGoogle Scholar
  20. Baydoun S, Lamis C, Helena D, Nelly A (2015) Ethnopharmacological survey of medicinal plants used in traditional medicine by the communities of Mount Hermon, Lebanon. J Ethnopharmacol 173:139–156PubMedCrossRefGoogle Scholar
  21. Bedir E, Khan IA, Walker LA (2002) Biologically active steroidal glycosides from Tribulus terrestris. Pharmazie 57:491–493PubMedGoogle Scholar
  22. Berger E (2001) The Canada Health Monitor surveys of health issues in Canada, Survey 22. Ottawa, ON, Health CanadaGoogle Scholar
  23. Betancur-Galvis LA, Morales GE, Forero JE, Roldan J (2002) Cytotoxic and antiviral activities of colombian medicinal plant extracts of the Euphorbia genus. Mem Inst Oswaldo Cruz 97:541–546PubMedCrossRefGoogle Scholar
  24. Billing J, Sherman PW (1998) Antimicrobial functions of spices: why some like it hot. Q Rev Biol 73(1):3–49PubMedCrossRefGoogle Scholar
  25. Blumenthal M, Busse WR, Goldberg A, Gruenwald J, Hall T, Riggins CW, Rister RS (eds) (1998) The complete German commission E monographs: therapeutic guide to herbal Medicines. American Botanical Council/Integrative Medicine Communications, Austin, TX/Boston, MAGoogle Scholar
  26. Bojadzievski P (1992) The health services in Bitola through the centuries. Society of Science and Art, BitolaGoogle Scholar
  27. Boonyaratanakornkit L, Chantaptavan V (1993) Identification and specification of Khao-Yen-Neua and Khao-Yen-Tai. Thai J Pharm Sci 1:79–90Google Scholar
  28. Botros L, Sakkas D, Seli E (2008) Metabolomics and its application for non-invasive embryo assessment in IVF. Mol Hum Reprod 14:679–690PubMedPubMedCentralCrossRefGoogle Scholar
  29. British Pharmacopoeia Commission (2007) British pharmacopoeia 2007. The Stationery Office, London. Accessed 16 July 2016
  30. Burslem DFRP, Garwood NC, Thomas SC (2001) Tropical forest diversity—the plot thickens. Science 291:606–607PubMedCrossRefGoogle Scholar
  31. Calapai G (2008) European legislation on herbal medicines: a look into the future. Drug Saf 31(5):428–431PubMedCrossRefGoogle Scholar
  32. Cancer Research UK (2014) A study looking at the use of herbal medicine by people with cancer. Accessed 16 July 2016
  33. Cancer Research UK (2016) About chemotherapy for lung cancer. Accessed 16 July 2016
  34. Castleman M (2001) The new healing herbs: the classic guide to nature’s best medicines featuring the top 100 time-tested herbs. Rodale Inc., New York, NYGoogle Scholar
  35. Cevallos-Cevallos JM, Futch DB, Shilts T, Folimonova SY, Reyes-De-Corcuera JI (2012) GC–MS metabolomic differentiation of selected citrus varieties with different sensitivity to citrus huanglongbing. Plant Physiol Biochem 53:69–76PubMedCrossRefGoogle Scholar
  36. Chaudhary A, Singh N (2010) Herbo mineral formulations (Rasaoushadhies) of Ayurvedia an amazing inheritance of Ayurvedic pharmaceutics. Anc Sci Life 30(1):18–26PubMedPubMedCentralGoogle Scholar
  37. Chayamarit K (1995) Thai medicinal plants, 5th edn. Department of Forestry, BangkokGoogle Scholar
  38. Costa-Lotufo LV, Khan MH, Ather A, Wilke DV, Jimenez PC, Pessoa C, Moraes ME (2005) Studies of the anticancer potential of plants used in Bangladeshi folk medicine. J Ethnopharmacol 99:21–30PubMedCrossRefGoogle Scholar
  39. Council of Europe, Strasburg. 2008. European pharmacopoeia. 6th edn., Accessed 16 July 2016
  40. Cox PA (2000) Will tribal knowledge survive the millennium? Science 287:44–45PubMedCrossRefGoogle Scholar
  41. Cragg GM, Newman DJ (2005) Plants as sources of anti-cancer agents. J Ethnopharmacol 100:72–79PubMedCrossRefGoogle Scholar
  42. Cunninghum AB (2001) Applied ethnobotany: people, wild plant use and conservation. Earthscan publications Ltd., LondonGoogle Scholar
  43. Dafni A, Levy S, Lev E (2005) The ethnobotany of Christʼs Thorne jujube (Ziziphus spina-christi) in Israel. J Ethnobiol Ethnomed 1:8PubMedPubMedCentralCrossRefGoogle Scholar
  44. Dervendzi V (1992) Contemporary treatment with medicinal plants. Tabernakul, SkopjeGoogle Scholar
  45. Duke JA, Ayensu ES (1985) Medicinal plants of China. Reference Publications, Inc., Algonac, MIGoogle Scholar
  46. Dunn WB, Ellis DI (2005) Metabolomics: current analytical platforms and methodologies. Trac Trends Anal Chem 24:285–294CrossRefGoogle Scholar
  47. Dwivedi G, Dwivedi S (2007) History of medicine: Sushruta – the clinician – teacher par excellence. Accessed 16 July 2016
  48. El Sheikha AF (2015a) New strategies of traceability for determining the geographical origin of medicinal plants: innovation of biological barcode by PCR-DGGE. ACMAP 5th Annual Conference Program book FARGO-ND. J Med Act Plants 4(2): Supplement. Accessed 16 July 2016
  49. El Sheikha AF (2004) Technological, chemical and microbiological studies on some packed foods. Dissertation, Minufiya UniversityGoogle Scholar
  50. El Sheikha AF (2010a) Determination of the geographical origin of fruits by using 26S rDNA fingerprinting of yeast communities by PCR-DGGE: an application to Shea tree fruits. J Life Sci 4(6):9–15Google Scholar
  51. El Sheikha AF (2010b) Determination of geographical origin of Shea tree and Physalis fruits by using the genetic fingerprints of the microbial community by PCR/DGGE: analysis of biological properties of some fruit extracts. Dissertation, Montpellier University IIGoogle Scholar
  52. El Sheikha AF (2011) Détermination de l’origine géographique des fruits: exemples du karité et du Physalis par l’utilisation d’empreintes génétiques sur la communauté microbienne par PCR/DGGE. In: Leclerc C (ed). Éditions Universitaire Européennes, GmbH & Co. KG, SarrebruckGoogle Scholar
  53. El Sheikha AF (2015b) Rumex nervosus: an overview. Int J Innov Hortic 4(2):87–95Google Scholar
  54. El Sheikha AF (2015c) New strategies for tracing foodstuffs: biological barcodes utilizing PCR-DGGE. Adv Food Technol Nutr Sci Open J 1(1):1–7CrossRefGoogle Scholar
  55. El Sheikha AF, Al-Sokari SS, Al-Ghamdi AY (2014) Ithrib (Rumex nervosus Vahl) as a famous ethno-medicinal plant of Saudi Arabia. In: 5th Annual Conference of the American Council for Medicinally Active Plants (ACMAP). 15–18 June 2014. Fargo, North Dakota, USAGoogle Scholar
  56. El Sheikha AF, Bouvet J-M, Montet D (2011a) Biological bar-code for the determination of geographical origin of fruits by using 28S rDNA fingerprinting of fungal communities by PCR-DGGE: an application to Shea tree fruits. Qual Assur Saf Crops Foods 3(1):40–47CrossRefGoogle Scholar
  57. El Sheikha AF, Condur A, Métayer I, Le Nguyen DD, Loiseau G, Montet D (2009) Determination of fruit origin by using 26S rDNA fingerprinting of yeast communities by PCR-DGGE: preliminary application to Physalis fruits from Egypt. Yeast 26:567–573PubMedCrossRefGoogle Scholar
  58. El Sheikha AF, Durand N, Sarter S, Okullo JBL, Montet D (2012) Study of the microbial discrimination of fruits by PCR-DGGE: application to the determination of the geographical origin of Physalis fruits from Colombia, Egypt, Uganda and Madagascar. Food Control 24(1–2):57–63CrossRefGoogle Scholar
  59. El Sheikha AF, Métayer I, Montet D (2011b) A Biological bar-code for determining the geographical origin of fruit by using 28S rDNA fingerprinting of fungi communities by PCR-DGGE: an application to Physalis fruits from Egypt. Food Biotechnol 25(2):115–129CrossRefGoogle Scholar
  60. El Sheikha AF, Montet D (2011) Determination of fruit origin by using 28S rDNA fingerprinting of fungi communities by PCR-DGGE: an application to Physalis fruits from Egypt, Uganda and Colombia. Fruits 66(2):79–89CrossRefGoogle Scholar
  61. El Sheikha AF, Montet D (2016) How to determine the geographical origin of seafood? Crit Rev Food Sci Nutr 56(2):306–317PubMedCrossRefGoogle Scholar
  62. El Sheikha AF, Ray R (2014) Is PCR-DGGE innovative molecular tool for detection of microbial plant pathogens? In: Sharma N (ed) Biological controls for preventing food deterioration. John Wiley & Sons, Ltd., ChichesterGoogle Scholar
  63. El Sheikha AF, Sainz de Los Terreros A, Nabet C, Montet D, Larroque M, Pelissier Y (2008a). Le Coqueret du Pérou (Physalis peruviana L.). 1st Prize of Ethnopharmacology and Ethnopharama laboratory. 10th International Symposium of Aromatherapy and Medicinal Plants, Grasse, 11–13 April 2008Google Scholar
  64. El Sheikha AF, Xu J (2017) Traceability as a key of seafood safety: reassessment and possible applications. Rev Fish Sci Aquac 25(2):158–170. doi: 10.1080/23308249.2016.1254158 CrossRefGoogle Scholar
  65. El Sheikha AF, Zaki M, Bakr A, El Habashy M, Montet D (2008b) Physico-chemical properties and biochemical composition of Physalis (Physalis pubescens L.) fruits. Global Science Books Ltd., UK. Food 2:124–130Google Scholar
  66. Ercolini D (2004) PCR-DGGE fingerprinting: novel strategies for detection of microbes in food. J Microbiol Methods 56:297–314PubMedCrossRefGoogle Scholar
  67. European Commission (2004) Directive 2004/24/EC of the European Parliament and of the Council of 31 March 2004 amending, as regards traditional herbal medicinal products, Directive 2001/83/EC on the Community code relating to medicinal products for human use. 30/04/20104. Official Journal of the European Union: L136/85. Accessed 16 July 2016
  68. Falodun A (2010) Herbalmedicine in Africa-distribution, standardization and prospects. Res J Phytochem 4(3):154–161CrossRefGoogle Scholar
  69. Farnsworth NR (1994) Ethnopharmacology and drug development. In: Prance GT (ed) Ethnobotany and the search for new drugs. Wiley, ChichesterGoogle Scholar
  70. Farnsworth NR, Bunyapraphatsara N (1992) Thai medicinal plant: recommended for primary health care system. Prachachon company, BangkokGoogle Scholar
  71. FDA (Food and Drug Administration) (2015) Botanical drug development guidance for industry. Accessed 16 July 2016
  72. Fischer SG, Lerman LS (1983) DNA fragments differing by single base-pair substitutions are separated in denaturing electrophoreseis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Proc Natl Acad Sci U S A 80(6):1579–1583PubMedPubMedCentralCrossRefGoogle Scholar
  73. Fouche G, Cragg GM, Pillay P, Kolesnikova N, Maharaj VJ, Senabe J (2008) In vitro anticancer screening of South African plants. J Ethnopharmacol 119:455–461PubMedCrossRefGoogle Scholar
  74. Gali-Muhtasib H, Diab-Assaf M, Boltze C, Al-Hmaira J, Hartig R, Roessner A, Schneider-Stock R (2004) Thymoquinone extracted from black seed triggers apoptotic cell death in human colorectal cancer cells via a p53-dependent mechanism. Int J Oncol 25:857–866PubMedGoogle Scholar
  75. GIA (Global Industry Analysts, Inc.) (2016) A worldwide business strategy & market intelligence source. Accessed 16 July 2016
  76. Gimmel M (2008) Reading medicine in the Codex de la Cruz Badiano. J Hist Ideas 69(2):169–192PubMedCrossRefGoogle Scholar
  77. Glaser V (1999) Billion-dollar market blossoms as botanicals take root. Nat Biotechnol 17:17–18PubMedCrossRefGoogle Scholar
  78. Gonsalves J (2010) Economic botany and ethnobotany. International Scientific Academy, DelhiGoogle Scholar
  79. Gurib-Fakim A (2006) Medicinal plants: traditions of yesterday and drugs of tomorrow. Mol Aspects Med 27(1):1–93PubMedCrossRefGoogle Scholar
  80. Harshaw D, Nahar L, Vadla B, Saif-E-Naser GM, Sarker SD (2010) Bioactivity of Rumex obtusifolius (Polygonaceae). Arch Biol Sci Belgrade 62(2):387–392CrossRefGoogle Scholar
  81. Hartwell JL (1982) Plants used against cancer. Quarterman, Lawrence, MAGoogle Scholar
  82. Hudaib M, Mohammad M, Bustanji Y, Tayyeb R, Yousef M, Aburjeie M, Aburjai T (2008) Ethnopharmacological survey of medicinal plants in Jordan, Mujib Nature Reserve and surrounding area. J Ethnopharmacol 120:63–71PubMedCrossRefGoogle Scholar
  83. Humber JM (2002) The role of complementary and alternative medicine: accommodating pluralism. JAMA 288(13):1655–1656CrossRefGoogle Scholar
  84. IARC (International Agency for Research on Cancer) (2002) Some traditional herbal medicines, some mycotoxins, naphthalene and styrene. IARC Monogr Eval Carcinog Risks Hum 82:1–556Google Scholar
  85. Ismail Z, Ismail N, Lassa J (1999) Malaysian herbal monograph, vol 1. Malaysian Monograph Committee, Kuala LumpurGoogle Scholar
  86. Itharat A, Houghton PJ, Eno-Amooquaye E, Burke PJ, Sampson J, Raman A (2004) In vitro cytotoxic activity of Thai medicinal plants used traditionally to treat cancer. J Ethnopharmacol 90:33–38PubMedCrossRefGoogle Scholar
  87. Itharat A, Singchangchai P, Subchareon P, Supavita T, Ubonkaw P, Ratanasuwan P, Kummee S, Srisuchatum P (2002) Folk wisdom about treatment of traditional doctors in Southern Thailand: focus groups discussion. Prince of Songkla University, SongklaGoogle Scholar
  88. Itharat A, Supavita T, Jusanit P, Singchangchai P, Subchareon P, Deevisad G, Ubonkaw P, Ratanasuwan P, Kajpunyapong V, Muangsrinun R (1999b) Survey of medicinal plants and local folk wisdom in Southern Thailand: case study at Talebun National Parks. Prince of Songkla University, SongklaGoogle Scholar
  89. Itharat A, Supavita T, Singchangchai P, Kajjarun U, Ratanasuwan P, Pongpeerachote S, Chantaptawan V (1999a) Identification and specification of Khao-Yen-Neua and Khao-Yen-Tai from traditional doctors in Thailand. Prince of Songkla University, SongklaGoogle Scholar
  90. Jacquart D (2008) Islamic pharmacology in the middle ages: theories and substances. Eur Rev 16(2):219–227CrossRefGoogle Scholar
  91. Jančić R (2002) Botanika farmaceutika. Public company Sl. List SRJ, BeogradGoogle Scholar
  92. Johnson T (2001) News. Chinese medicine now part of primary care scene in BC. Can Med Assoc J 164:1195Google Scholar
  93. Khan MY, Aliabbas S, Kumar V, Rajkumar S (2009) Recent advances in medicinal plant biotechnology. Indian J Biotechnol 8:9–22Google Scholar
  94. Kovacevic N (2000) Fundamentals of pharmacognosy. Personal edition, BeogradGoogle Scholar
  95. Kozyrskyj A (1997) Herbal products in Canada. How safe are they? Can. Family Phys Med Famille Can 43:697–702Google Scholar
  96. Krek M (1979) The enigma of the first Arabic book printed from movable type. J Near East Stud 38(3):203–212CrossRefGoogle Scholar
  97. Kuga H, Ejima A, Mitui I, Sato K, Ishihara N, Fukuda K, Saito F, Uenakai K (1993) Isolation and characterizacion of cytotoxic compounds from corn. Biosci Biotechnol Biochem 57(6):1020–1021. doi: 10.1271/bbb.57.1020 PubMedCrossRefGoogle Scholar
  98. Lambertini E, Piva R, Khan MTH, Lampronti I, Bianchi N, Borgatti M, Gambari R (2004) Effects of extracts from Bangladeshi medicinal plants on in vitro proliferation of human breast cancer cell lines and expression of estrogen receptor alpha gene. Int J Oncol 24:419–423PubMedGoogle Scholar
  99. Langmead L, Rampton DS (2001) Review article: herbal treatment in gastrointestinal and liver disease-benefits and dangers. Aliment Pharmacol Ther 15:1239–1252PubMedCrossRefGoogle Scholar
  100. Lee CC, Houghton P (2005) Cytotoxicity of plants from Malaysia and Thailand used traditionally to treat cancer. J Ethnopharmacol 100:237–243PubMedCrossRefGoogle Scholar
  101. Lee D-K, Lim DK, Um JA, Lim CJ, Hong JY, Yoon YA, Ryu Y, Kim HJ, Cho HJ, Park JH, Seo YB, Kim K, Lim J, Kwon SW, Lee J (2014) Evaluation of four different analytical tools to determine the regional origin of Gastrodia elata and Rehmannia glutinosa on the basis of metabolomics study. Molecules 19:6294–6308PubMedCrossRefGoogle Scholar
  102. Lee KH (1999) Antitumor agents 195 Anticancer drug design based on plant derived natural products. J Biomed Sci 6:236–250PubMedGoogle Scholar
  103. Leroi-Gourhan A (1975) The flowers found with Shanidar IV, a Neanderthal burial in Iraq. Science 190(4214):562–564CrossRefGoogle Scholar
  104. Li C, Yang S-C, Guo Q-S, Zheng K-Y, Shi Y-F, Xiao X-F, Long G-Q (2014) Determining the geographical origin of the medicinal plant Marsdenia tenacissima with multi-element analysis and data mining techniques. Chemom Intel Lab Syst 136:115–120CrossRefGoogle Scholar
  105. Li X-J, Zhang H-Y (2008) Western-medicine-validated anti-tumor agents and traditional Chinese medicine. Trends Mol Med 14(1):1–2PubMedCrossRefGoogle Scholar
  106. López-Rituerto E, Savorani F, Avenoza A, Busto JH, Peregrina JM, Engelsen SB (2012) Investigations of La Rioja terroir for wine production using 1H NMR metabolomics. J Agric Food Chem 60:3452–3461PubMedCrossRefGoogle Scholar
  107. Lukic P (1985) Pharmacognosy. SSO Faculty of Pharmacy, BeogradGoogle Scholar
  108. Luo F, Lu R, Zhou H, Hu F, Bao G, Huang B, Li Z (2013) Metabolic effect of an exogenous gene on transgenic Beauveria bassiana using Liquid Chromatography–Mass Spectrometry-based metabolomics. J Agric Food Chem 61:7008–7017PubMedCrossRefGoogle Scholar
  109. Madigan MT, Martinko JM, Dunlap PV, Clark DP (2009) Brock biology of microorganisms, 12th edn. Pearson Education Inc., San Francisco, CAGoogle Scholar
  110. Mamedov N (2012) Medicinal plants studies: history, challenges and prospective. Med Aromat Plants 1(8):e133. doi: 10.4172/2167-0412.1000e133 CrossRefGoogle Scholar
  111. Mekonnen T, Urga K, Engidawork E (2010) Evaluation of the diuretic and analgesic activities of the rhizomes of Rumex abyssinicus Jacq in mice. J Ethnopharmacol 127:433–439PubMedCrossRefGoogle Scholar
  112. Moco S, Vervoort J, Bino RJ, de Vos RCH, Bino R (2007) Metabolomics technologies and metabolite identification. Trac Trends Anal Chem 26:855–866CrossRefGoogle Scholar
  113. Moghadamtousi SZ, Rouhollahi E, Karimian H, Fadaeinasab M, Mohammad F, Ameen Abdulla M, Abdul Kadir H (2015) The chemopotential effect of Annona muricata leaves against azoxymethane-induced colonic aberrant crypt foci in rats and the apoptotic effect of acetogenin annomuricin E in HT-29 cells: a bioassay-guided approach. PLoS One 10(4):e0122288. doi: 10.1371/journal.pone.0122288 CrossRefGoogle Scholar
  114. Moreno-Escobar JA, Bazald S, Villarreal ML, Bonilla-Barbosa JR, Mendoza S, Rodríguez-López V (2011) Cytotoxic and antioxidant activities of selected Lamiales species from Mexico. Pharm Biol 49(12):1243–1248. doi: 10.3109/13880209.2011.589454 PubMedCrossRefGoogle Scholar
  115. Munavu RM, Mudamba LO, Ogur JA (1984) Isolation and characterization of the major anthraquinone pigments from Rumex abysinica. Planta Med 50(1):111. doi: 10.1055/s-2007-969640 PubMedCrossRefGoogle Scholar
  116. Munos B (2009) Lessons from 60 years of pharmaceutical innovation. Nat Rev Drug Discov 8:959–968PubMedCrossRefGoogle Scholar
  117. Muyzer G (1999) DGGE/TGGE a method for identifying genes from natural ecosystems. Curr Opin Microbiol 2(3):317–322PubMedCrossRefGoogle Scholar
  118. Muyzer G, De Waal EC, Uitterlinden AG (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol 59:695–700PubMedPubMedCentralGoogle Scholar
  119. Nair M, Nathan G (1998) Medicinal plants: cure for the 21th century; Biodiversity, conservation and utilization of Medicinal plants: Proceedings of the seminar UPM, Serdang, MalaysiaGoogle Scholar
  120. Nelson D, Cox M (2005) Lehninger principles of biochemistry, 4th edn. W.H. Freeman and Company, New York, NYGoogle Scholar
  121. Nganou DN, Durand N, Tatsadjieu NL, Meile JC, El Sheikha AF, Montet D, Mbufung CM (2012) Determination of coffee origin by using 28S rDNA fingerprinting of fungal communities by PCR-DGGE: application to the Cameroonian coffee. Int J Biosci 2:18–30Google Scholar
  122. Nisar MF, Jaleel F, Waseem M, Ismail S, Toor Y, Haider SM, Zhong JL (2014) Ethno-medicinal uses of plants from district Bahawalpur, Pakistan. Curr Res J Biol Sci 6(5):183–190Google Scholar
  123. Nunn JF (1996) Ancient Egyptian medicine. Trans Med Soc Lond 113:57–68PubMedGoogle Scholar
  124. Oakes L, Gahlin L (2003) Ancient Egypt: an illustrated reference to the myths, religions, pyramids and temples of the land of the pharaohs. Barnes & Noble, New York, NYGoogle Scholar
  125. Oran SA, Al-Eisawi DM (1998) Check list of medicinal plants in Jordan. Dirasat 25:84–112Google Scholar
  126. Padulosi S, Leaman D, Quek P (2002) Challenges and opportunities in enhancing the conservation and use of medicinal and aromatic plants. J Herbs Spices Med Plants 9:243–267CrossRefGoogle Scholar
  127. Pan S-Y, Litscher G, Gao S-H, Zhou S-F, Yu Z-L, Chen H-Q, Zhang S-F, Tang M-K, Sun J-N, Ko K-M (2014) Historical perspective of traditional indigenous medical practices: the current renaissance and conservation of herbal resources. Evid Based Complement Alternat Med 2014:525340. doi: 10.1155/2014/525340 PubMedPubMedCentralGoogle Scholar
  128. Pan S-Y, Pan S, Yu Z-L, Ma D-L, Chen S-B, Fong W-F, Han Y-F, Ko K-M (2010) New perspectives on innovative drug discovery: an overview. J Pharm Pharm Sci 13(3):450–471PubMedCrossRefGoogle Scholar
  129. Petrovska BB (2012) Historical review of medicinal plants’ usage. Pharmacogn Rev 6(11):1–5PubMedPubMedCentralCrossRefGoogle Scholar
  130. Popoca J, Aguilar A, Alonso D, Villarreal ML (1998) Cytotoxic activity of selected plants used as antitumorals in Mexican traditional medicine. J Ethnopharmacol 59:173–177. doi: 10.1016/S0378-8741(97)00110-4 PubMedCrossRefGoogle Scholar
  131. Rajeshkumar NV, Pillai MR, Kuttan R (2003) Induction of apoptosis in mouse and human carcinoma cell lines by Emblica officinalis polyphenols and its effect on chemical carcinogenesis. J Exp Clin Res 22:201–212Google Scholar
  132. Rao SR, Ravishankar GA (2002) Plant cell cultures: chemical factories of secondary metabolites. Biotechnol Adv 20:101–153PubMedCrossRefGoogle Scholar
  133. Rates SMK (2001) Plants as source of drugs. Toxicon 39:603–613PubMedCrossRefGoogle Scholar
  134. Rettedal EA, Clay S, Brözel VS (2010) GC-clamp primer batches yield 16S rRNA gene amplicon pools with variable GC clamps, affecting denaturing gradient gel electrophoresis profiles. FEMS Microbiol Lett 312(1):55–62PubMedCrossRefGoogle Scholar
  135. Ríos MY, González-Morales A, Villarreal ML (2001) Sterols, triterpenes and bioflavonoids of Viburnum jucundum and cytotoxic activity of ursolic acid. Planta Med 67:683–684. doi: 10.1055/s-2001-17357 PubMedCrossRefGoogle Scholar
  136. Robson B, Baek OK (2009) The engines of hippocrates: from the dawn of medicine to medical and pharmaceutical informatics. John Wiley & Sons, Hoboken, NJCrossRefGoogle Scholar
  137. Rojas-Sepúlveda A, Mendieta-Serrano M, Antúnez Mojica M, Salas-Vidal E, Marquina S, Villarreal ML, Puebla AM, Delgado J, Alvarez L (2012) Cytotoxic podophyllotoxin type-lignans from the steam bark of Bursera fagaroides var. fagaroides. Molecules 17:9506–9519. doi: 10.3390/molecules17089506 PubMedCrossRefGoogle Scholar
  138. Roy MK, Nakahara K, Na Thalang V, Trakoontivakorn G, Takenaka M, Isobe S, Tsushida T (2007) Baicalein, a flavonoid extracted from a methanolic extract of Oroxylum indicum inhibits proliferation of a cancer cell line in vitro via induction of apoptosis. Pharmazie 62:149–153PubMedGoogle Scholar
  139. Saad B, Azaizeh H, Said O (2005) Tradition and perspectives of Arab herbal medicine: a review. Evid Based Complement Alternat Med 2(4):475–479PubMedPubMedCentralCrossRefGoogle Scholar
  140. Said O, Fulder S, Khalil K, Eli K, Bashar S (2008) Maintaining a Physiological Blood Glucose Level with ‘Glucolevel’, a combination of four anti-diabetes plants used in the traditional Arab herbal medicine. eCAM 5(4):421–428PubMedGoogle Scholar
  141. Said O, Khalil K, Fulder S, Azaizeh H (2002) Ethnopharmacological survey of medicinal herbs in Israel, the Golan Heights and the West Bank region. J Ethnopharmacol 83:251–265PubMedCrossRefGoogle Scholar
  142. Saied AS, Gebauer J, Hammer K, Buerkert A (2008) Ziziphus spina-christi (L.) Willd: a multipurpose fruit tree. Genet Resour Crop Evol 55:929–937CrossRefGoogle Scholar
  143. Sawahel W (1997) Plant genetic transformation technology. Daya Publishing House, DelhiGoogle Scholar
  144. Seid MA, Aydagnehum SG (2013) Medicinal plants biodiversity and local healthcare management system in Chencha district; Gamo Gofa, Ethiopia. J Pharmacogn Phytochem 2(1):284–293Google Scholar
  145. Shafik A, Elseesy W (2003) Medicine in ancient Egypt. In: Seli H, Shapiro H (eds) Medicine across cultures. Kluwer, Boston, MAGoogle Scholar
  146. Sherman PW, Hash GA (2001) Why vegetable recipes are not very spicy. Evol Hum Behav 22(3):147–163PubMedCrossRefGoogle Scholar
  147. Shetty P, Rinaldi A (2015) Traditional medicine for modern times: facts and figures. Accessed 16 July 2016
  148. Shi G, MacDougal JM, McLaughlin JL (1997) Bioactive annonaceous acetogenins from Rollinia mucosa. Phytochemistry 45:719–723. doi: 10.1016/S0031-9422(97)00028-9 PubMedCrossRefGoogle Scholar
  149. Siahsar B, Rahimi M, Tavassoli A, Raissi A (2011) Application of biotechnology in production of medicinal plants. Am Eur J Agric Environ Sci 11(3):439–444Google Scholar
  150. Sigerist HE (1967) A history of medicine, I. Primitive and archaic medicine. Oxford University Press, New York, NYGoogle Scholar
  151. Sonnedecker G, Kremers E (1986) Kremers and Urdang’s history of pharmacy. American Institute of the History of Pharmacy, Madison, WIGoogle Scholar
  152. Stepp JR (2004) The role of weeds as sources of pharmaceuticals. J Ethnopharmacol 92(2–3):163–166PubMedCrossRefGoogle Scholar
  153. Sumner J (2000) The natural history of medicinal plants. Timber Press, Portland, ORGoogle Scholar
  154. Sun B, Qu W, Bai Z (2003) The inhibitory effect of saponins from Tribulus terrestris on Bcap-37 breast cancer cell line in vitro. Zhong Yao Cai 26:104–106PubMedGoogle Scholar
  155. Talib WH, Mahasneh AM (2010) Antiproliferative activity of plant extracts used against cancer in traditional medicine. Sci Pharm 78:33–45PubMedPubMedCentralCrossRefGoogle Scholar
  156. Tamokou J d D, Chouna JR, Fischer-Fodor E, Chereches G, Barbos O, Damian G, Benedec D, Duma M, APN E, Wabo HK, Kuiate JR, Mot A, Silaghi-Dumitrescu R (2013) Anticancer and antimicrobial activities of some antioxidant-rich Cameroonian medicinal plants. PLoS One 8(2):e55880. doi: 10.1371/journal.pone.0055880 PubMedCentralCrossRefGoogle Scholar
  157. Tasheva K, Kosturkova G (2013) Role of biotechnology for protection of endangered medicinal plants. In: Petre M (ed) Environmental biotechnology - new approaches and prospective applications. InTech, RijekaGoogle Scholar
  158. The United States Pharmacopoeial Convention. Washington: 2008. USP 31 the United States Pharmacopoeia. Accessed 16 July 2016
  159. Toplak Galle K (2005) Domestic medicinal plants. Mozaic book, ZagrebGoogle Scholar
  160. Truitt ER (2009) The virtues of balm in late medieval literature. Early Sci Med 14(6):711–736PubMedCrossRefGoogle Scholar
  161. Tzfira T, Li J, Lacroix B, Citovsky V (2004) Agrobacterium tDNA integration: molecules and models. Trends Genet 20:375–383PubMedCrossRefGoogle Scholar
  162. Vaz-Moreira I, Egas C, Nunes OC, Manaia CM (2013) Bacterial diversity from the source to the tap: a comparative study based on 16S rRNA gene-DGGE and culture-dependent methods. FEMS Microbiol Ecol 83(2):361–374PubMedCrossRefGoogle Scholar
  163. Vega-Ávila E, Tapia Aguilar R, Jiménez Estrada M, Villareal Ortega M, Román-Ramos R (2004) Cytotoxic activity of Cuphea aequipetala. Proc West Pharmacol Soc 47:129–133. doi: 10.1016/j.jep.2010.11.055 Google Scholar
  164. Vergouw CG, Botros LL, Roos P, Lens JW, Schats R, Hompes PGA, Burns DH, Lambalk CB (2008) Metabolomic profiling by near-infrared spectroscopy as a tool to assess embryo viability: a novel, non-invasive method for embryo selection. Hum Reprod 23:1499–1504PubMedCrossRefGoogle Scholar
  165. Von Staden H (1989) Herophilus: the art of medicine in early Alexandria: edition, translation, and essays. Cambridge University Press, New York, NYGoogle Scholar
  166. Wang J-B, Zeng L-N, Zang Q-C, Gong Q-F, Li B-C, Zhang X-R, Chu X-H, Zhang P, Zhao Y-L, Xiao X-H (2012) Colorimetric grading scale can promote the standardization of experiential and sensory evaluation in quality control of traditional Chinese medicines. PLoS One 7:e48887. doi: 10.1371/journal.pone.0048887 PubMedPubMedCentralCrossRefGoogle Scholar
  167. WHO (2013) WHO traditional medicine strategy: 2014-2023. Accessed 16 July 2016
  168. WHO (World Health Organization) (1996) Annex II. Guidelines for the assessment of herbal medicines. (WHO Technical Report Series No. 863), GenevaGoogle Scholar
  169. Wieczorek A (2003) Use of biotechnology in agriculture—benefits and risks. Governance for industrial transformation. Proceedings of the 2003 Berlin conference on the human resources. Honolulu, HI: University of Hawaii Press. Accessed 16 July 2016
  170. Williamson E, Okpako DT, Evans FJ (1996) Selection, preparation and pharmacological evaluation of plant material. Wiley, ChichesterGoogle Scholar
  171. Wu J-N (2005) An illustrated Chinese materia medica. Oxford University Press, New York, NYGoogle Scholar

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© Springer International Publishing AG 2017

Authors and Affiliations

  1. 1.Department of BiologyMcMaster UniversityHamiltonCanada
  2. 2.Department of Food Science and Technology, Faculty of AgricultureMinufiya UniversityShibin El KomEgypt

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