Lab-on-a-Chip Proteomic Assays for Psychiatric Disorders

  • Harald PeterEmail author
  • Julia Wienke
  • Paul C. Guest
  • Nikitas Bistolas
  • Frank F. Bier
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 974)


Lab-on-a-chip assays allow rapid identification of multiple parameters on an automated user-friendly platform. Here we describe a fully automated multiplex immunoassay and readout in less than 15 min using the Fraunhofer in vitro diagnostics (ivD) platform to enable inexpensive point-of-care profiling of sera or a single drop of blood from patients with various diseases such as psychiatric disorders.


Psychiatric disorders Early diagnosis Proteomics Biomarkers Immunoassay point-of-care Lab-on-a-chip 


  1. 1.
    Olesen J, Gustavsson A, Svensson M, Wittchen HU, Jönsson B, CDBE2010 study group (2012) The economic cost of brain disorders in Europe. Eur J Neurol 19:155–162CrossRefPubMedGoogle Scholar
  2. 2.
    Mitchell AJ, Vaze A, Rao S (2009) Clinical diagnosis of depression in primary care: a meta-analysis. Lancet 374:609–619CrossRefPubMedGoogle Scholar
  3. 3.
    Bet PM, Hugtenburg JG, Penninx BW, Av B, Nolen WA, Hoogendijk WJ (2013) Treatment inadequacy in primary and specialized care patients with depressive and/or anxiety disorders. Psychiatry Res 210:594–600CrossRefPubMedGoogle Scholar
  4. 4.
    American Psychiatric Association (2013) Diagnostic and statistical manual of mental disorders, Fifth Edition (DSM-5), 5th edn. American Psychiatric Publishing, Washington ISBN-13: 978–0890425558Google Scholar
  5. 5.
  6. 6.
    Szulc A, Galińska B, Konarzewska B, Gudel-Trochimowicz I, Popławska R (2001) Immunological marker activity in first episode schizophrenic patients. Pol Merkur Lekarski 10:450–452PubMedGoogle Scholar
  7. 7.
    Van Venrooij JA, Fluitman SB, Lijmer JG, Kavelaars A, Heijnen CJ, Westenberg HG et al (2012) Impaired neuroendocrine and immune response to acute stress in medication-naive patients with a first episode of psychosis. Schizophr Bull 38:272–279CrossRefPubMedGoogle Scholar
  8. 8.
    Perkins DO, Jeffries CD, Addington J, Bearden CE, Cadenhead KS, Cannon TD et al (2014) Towards a psychosis risk blood diagnostic for persons experiencing high-risk symptoms: preliminary results from the NAPLS project. Schizophr Bull 41:419–428CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Chan MK, Krebs MO, Cox D, Guest PC, Yolken RH, Rahmoune H et al (2015) Development of a blood-based molecular biomarker test for identification of schizophrenia before disease onset. Transl Psychiatry 5:e601. doi: 10.1038/tp.2015.9 CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Schwarz E, Izmailov R, Spain M, Barnes A, Mapes JP, Guest PC et al (2010) Validation of a blood-based laboratory test to aid in the confirmation of a diagnosis of schizophrenia. Biomark Insights 5:39–47PubMedPubMedCentralGoogle Scholar
  11. 11.
    Schwarz E, Guest PC, Rahmoune H, Harris LW, Wang L, Leweke FM et al (2012) Identification of a biological signature for schizophrenia in serum. Mol Psychiatry 17:494–502CrossRefPubMedGoogle Scholar
  12. 12.
    Papakostas GI, Shelton RC, Kinrys G, Henry ME, Bakow BR, Lipkin SH et al (2013) Assessment of a multi-assay, serum-based biological diagnostic test for major depressive disorder: a pilot and replication study. Mol Psychiatry 18:332–339CrossRefPubMedGoogle Scholar
  13. 13.
    Gottschalk MG, Cooper JD, Chan MK, Bot M, Penninx BW, Bahn S (2015) Discovery of serum biomarkers predicting development of a subsequent depressive episode in social anxiety disorder. Brain Behav Immun 48:123–131CrossRefPubMedGoogle Scholar
  14. 14.
    Gottschalk MG, Cooper JD, Chan MK, Bot M, Penninx BW, Bahn S et al (2016) Serum biomarkers predictive of depressive episodes in panic disorder. J Psychiatr Res 73:53–62CrossRefPubMedGoogle Scholar
  15. 15.
    Schwarz E, Guest PC, Steiner J, Bogerts B, Bahn S (2012) Identification of blood based molecular signatures for prediction of response and relapse in schizophrenia patients. Transl Psychiatry 2:e82CrossRefPubMedPubMedCentralGoogle Scholar
  16. 16.
    Tomasik J, Schwarz E, Lago SG, Rothermundt M, Leweke FM, van Beveren NJ et al (2016) Pretreatment levels of the fatty acid handling proteins H-FABP and CD36 predict response to olanzapine in recent-onset schizophrenia patients. Brain Behav Immun 52:178–186CrossRefPubMedGoogle Scholar
  17. 17.
    Bilello JA, Thurmond LM, Smith KM, Pi B, Rubin R, Wright SM et al (2015) MDDScore: confirmation of a blood test to aid in the diagnosis of major depressive disorder. J Clin Psychiatry 76:e199–e206CrossRefPubMedGoogle Scholar
  18. 18.
    Wehler CA, Preskorn SH (2016) High false-positive rate of a putative biomarker test to aid in the diagnosis of schizophrenia. J Clin Psychiatry 77:e451–e456CrossRefPubMedGoogle Scholar
  19. 19.
  20. 20.
  21. 21.
    Schumacher S, Nestler J, Otto T, Wegener M, Ehrentreich-Förster E, Michel D et al (2012) Highly-integrated lab-on-chip system for point-of-care multiparameter analysis. Lab Chip 12:464–473CrossRefPubMedGoogle Scholar
  22. 22.
    Schumacher S, Ludecke C, Ehrentreich-Förster E, Bier FF (2013) Platform technologies for molecular diagnostics near the patient's bedside. Adv Biochem Eng Biotechnol 133:75–87PubMedGoogle Scholar
  23. 23.
    Streit P, Nestler J, Shaporin A, Schulze R, Gessner T (2016) Thermal design of integrated heating for lab-on-a-chip systems. Proceedings of the 17th international conference on thermal, mechanical and multi-physics simulation and experiments in microelectronics and microsystems (EuroSimE). pp 1–6Google Scholar

Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  • Harald Peter
    • 1
    Email author
  • Julia Wienke
    • 1
  • Paul C. Guest
    • 2
  • Nikitas Bistolas
    • 1
  • Frank F. Bier
    • 1
  1. 1.Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses (IZI-BB)PotsdamGermany
  2. 2.Laboratory of Neuroproteomics, Department of Biochemistry and Tissue BiologyInstitute of Biology, University of Campinas (UNICAMP)CampinasBrazil

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