Abstract
Mussels possess strong underwater adhesion on different substrata due to unique adhesive property of dihydroxyphenylalanine (DOPA) -rich mussel adhesive proteins (MAPs). MAPs have emerged as new biomaterials for broad applications ranging from drug delivery and medical device coating to bleeding control and wound healing . After an overview of MAPs, this chapter summarizes MAP-based commercial products and their application. Two production methods of MAPs, natural extraction and recombinant production , are then reviewed and compared in detail. To date, MAPs extracted from mussels show better adhesive properties, but recombinant production offers a low-cost approach with advantages including engineering additional functions. Modifications and formulations of MAPs, particularly for recombinant MAPs, are essential to ensure high performance of MAP-based products. Different approaches including tyrosinase-based enzyme modification, metal-mediated cross-linking, and coacervation of MAPs with anionic molecules have been used to enhance MAPs’ functions. After an overview on production methods, this chapter provides literature sources for adhesive mechanism, characterization techniques, and broad application of MAPs.
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Castillo, J.J., Shanbhag, B.K., He, L. (2017). Comparison of Natural Extraction and Recombinant Mussel Adhesive Proteins Approaches. In: Puri, M. (eds) Food Bioactives. Springer, Cham. https://doi.org/10.1007/978-3-319-51639-4_5
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