Literature Review: What Has Been Explored About Enzymatic Synthesis of ST and SD?

Part of the SpringerBriefs in Molecular Science book series (BRIEFSMOLECULAR)


This chapter focuses on the enzymatic synthesis of triglycerides and diglycerides in one or more steps. It also discusses the attempts to introduce chemical steps to reduce relative costs. The main costs in practical, industrial environments are the solvent and the enzymes. The reactions are studied in either solvent or solvent-free media, especially in the last 5 years.

The main substrates are glycerol and vegetable oils. There is a trend in using biomass or waste from other industries instead of edible oils, especially due to the impact of using high-value nutritional oils as a source. In this sense, the best option seems to be the use of glycerol, a low-cost source of acyl groups and a low-cost source of lipase, and to avoid solvents. However, with the goal of a high conversion of glycerol to the required structured di- and triglycerides, this is not an easy task. Problems like lipase inhibition by glycerol or acyl compounds, the required immobilization of the enzyme, the needed reuse of the immobilized lipase and an adequate level of mixing to achieve the required reaction media are being increasingly studied. Strategies to improve reuse, enzymatic activity in reuse and stability in storage are being more and more explored.

The review is thought to cover mainly the last 5–10 years with the most important advances in context, especially in the field of immobilized enzyme applications and other post-immobilization treatments.


Glycerolysis Esterification Hydrolysis Interesterification Chemo-enzymatic Multistep synthesis 


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Copyright information

© The Author(s) 2017

Authors and Affiliations

  1. 1.PLAPIQUI-UNS-CONICETBuenos AiresArgentina

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