Skip to main content
SpringerLink
Log in

Promising Test Systems Beyond the Current Status

  • Chapter
  • First Online:
Alternatives for Dermal Toxicity Testing

  • 696 Accesses

Abstract

In addition to the mature animal-free testing methods for skin sensitization, several promising new test systems or modifications of existing systems are emerging. This chapter provides a non-exhaustive list of emerging tools for animal-free assessment of skin sensitization addressing skin bioavailability, haptenation, inflammatory mechanisms and dendritic cell activation, and migration. The exact added value of these emerging tools remains to be substantiated.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
eBook
USD 16.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Groeber F, Engelhardt L, Egger S, Werthmann H, Monaghan M, Walles H, Hansmann J. Impedance spectroscopy for the non-desctructive evaluation of in vitro epidermal models. Pharm Res. 2014;32(5):1845–54. doi:10.1007/s11095-014-1580-3.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  2. Brauchle E, Schenke-Layland K. Raman spectroscopy in biomedicine – non-invasive in vitro analysis of cells and extracelluilar matrix components in tissues. Biotechnol J. 2013;8(3):288–97. doi:10.1002/biot.201200163.

    Article  PubMed  CAS  Google Scholar 

  3. O’Brien PJ, Irwin W, Diaz D, Howard-Cofield E, Krejsa CM, Slaughter MR, Gao B, Kaludercic N, Angeline A, Bernardi P, Brain P, Hougham C. High concordance of drug-induced human hepatotoxicity with in vitro cytotoxicity measured in a novel cell-based model using high content screening. Arch Toxicol. 2006;80(9):580–604.

    Article  PubMed  CAS  Google Scholar 

  4. Tolosa L, Pinto S, Donato MT, Lahoz A, Castell JV, O’Connor JE, Gómez-Lechón MJ. Development of a multiparametric cell-based protocol to screen and classify the hepatoxicity potential of drugs. Toxicol Sci. 2012;127:187–98.

    Article  PubMed  CAS  Google Scholar 

  5. Manchanda T, Hess D, Dale L, Ferguson SG, Rieder MJ. Haptenation of sulfonamide reactive metabolites to cellular proteins. Mol Pharmacol. 2002;62:1011–26.

    Article  PubMed  CAS  Google Scholar 

  6. Dietz L, Kinzebach S, Ohnesorge S, Franke B, Goette I, Koenig-Gressel D, et al. Proteomic allergen-peptide/protein interaction assay for the identification of human skin sensitizers. Toxicol In Vitro. 2013;27:1157–62. doi:10.1016/j.tiv.2012.08.013.

    Article  PubMed  CAS  Google Scholar 

  7. Natsch A, Gfeller H. LC-MS-based characterization of the peptide reactivity of chemicals to improve the in vitro prediction of the skin sensitization potential. Toxicol Sci. 2008;106(2):464–78.

    Article  CAS  PubMed  Google Scholar 

  8. Yamamoto Y, Tahara H, Usami R, Kasahara T, Jimbo Y, Hioki T, Fujita M. A novel in chemico method to detect skin sensitizers in highly diluted reaction conditions. J Appl Toxicol. 2015;35:1348–60.

    Article  CAS  PubMed  Google Scholar 

  9. Aptula AO, Patlewicz G, Roberts DW, Schultz TW. Non-enzymatic glutathione reactivity and in vitro toxicity: a non-animal approach to skin sensitization. Toxicol In Vitro. 2006;20:239–47.

    Article  CAS  PubMed  Google Scholar 

  10. Jacquoilleot S, Sheffield D, Olayanju A, Sison-Young R, Kitteringham NR, Naisbitt DJ, Aleksic M. Glutathione metabolism in the HaCaT cell line as a model for the detoxification of the model sensitizers 2,4-dinitrohalobenzenes in human skin. Toxicol Lett. 2015;237:11–20.

    Article  CAS  PubMed  Google Scholar 

  11. Saito K, Nukada Y, Takenouchi O, Miyazawa M, Sakaguchi H, Nishiyama N. Development of a new in vitro skin sensitization assay (epidermal sensitization assay; EpiSensA) using reconstructed human epidermis. Toxicol In Vitro. 2013;27:2213–24.

    Article  CAS  PubMed  Google Scholar 

  12. van der Veen JW, Pronk TE, van Loveren H, Ezendam J. Applicability of a keratinocyte gene signature to predict skin sensitizing potential. Toxicol In Vitro. 2013;27:314–22.

    Article  CAS  PubMed  Google Scholar 

  13. Nukada Y, Miyazawa M, Kosaka N, Ito Y, Sakaguchi H, Nishiyama N. Production of IL-8 in THP-1 cells following contact allergen stimulation via mitogen-activated protein kinase activation or tumor necrosis factor-alpha production. J Toxicol Sci. 2008;33:175–85.

    Article  CAS  PubMed  Google Scholar 

  14. Python F, Goebel C, Aeby P. Assessment of the U937 cell line for the detection of contact allergens. Toxicol Appl Pharmacol. 2007;220:113–24.

    Article  CAS  PubMed  Google Scholar 

  15. Toebak MJ, Gibbs S, Bruynzeel DP, Scheper RJ, Rustemeyer T. Dendritic cells: biology of the skin. Contact Dermatitis. 2009;60:2–20.

    Article  CAS  PubMed  Google Scholar 

  16. Takahashi T, Kimura Y, Saito R, Nakajima Y, Ohmiya Y, Yamasaki K, Aiba S. An in vitro test to screen skin sensitizers using a stable THP-1-derived IL-8 reporter cell line, THP-G8. Toxicol Sci. 2011;124(2):359–69.

    Article  CAS  PubMed  Google Scholar 

  17. Villablanca EJ, Russo V, Mora JR. Dendritic cell migration and lymphocyte homing imprinting. Histol Histopathol. 2008;23:897–910.

    PubMed  CAS  Google Scholar 

  18. Ouwehand K, Scheper RJ, de Gruijl TD, Gibbs S. Epidermis-todermis migration of immature Langerhans cells upon topical irritant exposure is dependent on CCL2 and CCL5. Eur J Immunol. 2012;40:2026–34.

    Article  CAS  Google Scholar 

  19. Ouwehand K, Spiekstra SW, Waaijman T, Scheper RJ, de Gruijl TD, Gibbs S. Langerhans cells derived from a human cell line in a full-thickness skin equivalent undergo allergen-induced maturation and migration. Tech Adv. 2011;90:1028–33.

    Google Scholar 

  20. Rees B, Spiekstra SW, Carfi M, Ouwehand K, Williams CA, Corsini E, McLeod JD, Gibbs S.Inter-laboratory study of the in vitro dendritic cell migration assay for identification of contact allergens. Toxicol In Vitro. 2011;25:2124–34.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. 3Rs Management and Consulting ApS, Kongens Lyngby, Denmark

    Erwin L. Roggen

Authors
  1. Erwin L. Roggen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Erwin L. Roggen .

Editor information

Editors and Affiliations

  1. SeCAM, Magliaso, Switzerland

    Chantra Eskes

  2. Innovitox Consulting & Services, Houten, The Netherlands

    Erwin van Vliet

  3. Department of Dermatology, University of California School of Medicine, San Francisco, California, USA

    Howard I. Maibach

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer International Publishing AG

About this chapter

Cite this chapter

Roggen, E.L. (2017). Promising Test Systems Beyond the Current Status. In: Eskes, C., van Vliet, E., Maibach, H. (eds) Alternatives for Dermal Toxicity Testing. Springer, Cham. https://doi.org/10.1007/978-3-319-50353-0_32

Download citation

  • DOI: https://doi.org/10.1007/978-3-319-50353-0_32

  • Published:

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-50351-6

  • Online ISBN: 978-3-319-50353-0

  • eBook Packages: MedicineMedicine (R0)

Publish with us

Policies and ethics

Search

Navigation