GARD: Genomic Allergen Rapid Detection

  • Malin Lindstedt
  • Kathrin Stephanie Zeller
  • Henrik Johansson
  • Carl Borrebaeck
Chapter

Abstract

Genomic allergen rapid detection (GARD) is an in vitro assay for the prediction of skin sensitizing capacity of chemicals. The GARD assay monitors changes in the expression of 196 genes, termed the GARD prediction signature. In short, MUTZ-3 cells, representing a well-characterized in vitro model of human dendritic cells (DCs), are exposed to the test substances for 24 h. After RNA extraction and analysis of expression levels, compounds are predicted as either sensitizers or non-sensitizers by a support vector machine model. Due to high information content, the test can deliver novel insights into pathway utilization and potency. Based on blinded test evaluations, GARD has been shown to have an accuracy of 89%.

Keywords

Dendritic cells Sensitization Human In vitro assay Biomarker signatures 

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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  • Malin Lindstedt
    • 1
  • Kathrin Stephanie Zeller
    • 1
  • Henrik Johansson
    • 2
  • Carl Borrebaeck
    • 1
  1. 1.Department of ImmunotechnologyLund UniversityLundSweden
  2. 2.SenzaGen ABLundSweden

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