Chlorophyll Fluorescence for High-Throughput Screening of Plants During Abiotic Stress, Aging, and Genetic Perturbation
Chlorophyll (Chl) is nature’s gift to oxygenic photosynthetic organisms which capture solar radiation and convert it into chemical energy to drive the whole process of photosynthesis for proper growth and development of plants. Understanding the responses of photosynthetic apparatus in crop plants under various stress conditions has become a major target for many research programs. In this chapter, we describe the principal of Chl fluorescence and the recent advances in the application of Chl fluorescence. Chl fluorescence measurement is one of the most useful, cost-effective, and non-invasive tools to measure efficiency of photosystem II photochemistry. Incorporated with improved imaging and computer technologies, it can be utilized on a small or large scale for examination of photosynthetic performance, stress tolerance, and aging. Further advancements are being made to develop efficient more tools to apply Chl fluorescence measurement for large-scale high-throughput photosynthesis phenotyping, forestry and crop management.