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Fluorescence Studies for Biomolecular Structure and Dynamics

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Reviews in Fluorescence 2016

Part of the book series: Reviews in Fluorescence ((RFLU))

Abstract

Bio-molecular structure and dynamics have been studied using different fluorescence monitoring methods. In the present review, we have discuss the principle and application of different methods used to monitor conformation and dynamics of proteins (beta-lactamase, barnase, Ribonuclease) and DNA (duplex, triplex and quadruple), and macromolecules assembly (membrane) etc. We have also discussed the rules that are used to monitor the conformation of biomolecules using intrinsic and extrinsic fluorescence as well as selective monitoring of surface tryptophan of protein (beta-lactamase) using excitation at 305 nm. Therefore, the present fluorescence studies will be useful to monitor the bio-molecular structure and dynamics.

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Acknowledgement

VT would like to thanks SERB, DST, India for Start Up grant (SB/YS/LS-07/2014). VS would like to thanks UGC for the JRF fellowship.

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The authors have declared that no competing interests exist.

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Correspondence to Vishvanath Tiwari .

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Tiwari, V., Solanki, V. (2017). Fluorescence Studies for Biomolecular Structure and Dynamics. In: Geddes, C. (eds) Reviews in Fluorescence 2016. Reviews in Fluorescence. Springer, Cham. https://doi.org/10.1007/978-3-319-48260-6_12

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