Skip to main content

Abstract

Platelet adhesion is a critical functional component of blood platelets in pathophysiology and a current focus of drugs modulating platelet activity. In this chapter, we discuss platelet adhesion in terms of both basic science and clinical relevance, not only related to thrombosis and haemostasis, but also in terms of inflammation, infection, and immune-related functions of platelets. In particular, a key factor in the initiation and control of platelet adhesion in the healthy or diseased bloodstream is the effect of changes in fluid shear forces, which is also essential in control of expression and regulation of platelet-specific receptors such as glycoprotein (GP) Ib-IX-V and GPVI, and the interactions with their major binding partners, including von Willebrand factor (VWF) and collagen, respectively. It is the nature of human platelets to rapidly become adhesive to various cellular or non-cellular substrates and to secrete an array of prothrombotic and proinflammatory factors, to localize coagulation and neutrophil-mediated inflammation within the vasculature. Understanding the cellular, biochemical, and shear-related mechanisms involved in platelet adhesion can underpin new advances in the diagnosis and therapy of disease.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 299.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 379.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 379.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Alshehri OM, Hughes CE, Montague S, Watson SK, Frampton J, Bender M, Watson SP (2015) Fibrin activates GPVI in human and mouse platelets. Blood 126(13):1601–1608

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Al-Tamimi M, Tan CW, Qiao J, Pennings GJ, Javadzadegan A, Yong ASC, Arthur JF, Davis AK, Jing J, Mu F-T, Hamilton JR, Jackson SP, Ludwig A, Berndt MC, Ward CM, Kritharides L, Andrews RK, Gardiner EE (2012) Pathological shear triggers shedding of vascular receptors: a novel mechanism for downregulation of platelet glycoprotein (GP)VI in stenosed coronary vessels. Blood 119(18):4311–4320

    Article  CAS  PubMed  Google Scholar 

  • Andrews RK, Berndt MC (2008) Platelet adhesion: a game of catch and release. J Clin Invest 118(9):3009–3011

    CAS  PubMed  PubMed Central  Google Scholar 

  • Andrews RK, Berndt MC (2012) The glycoprotein Ib-IX-V complex. In: Michelson A (ed) Platelets, 3rd edn. Academic Press, San Diego, pp 195–211

    Google Scholar 

  • Andrews RK, Berndt MC (2013) Bernard-Soulier syndrome: an update. Semin Thromb Hemost 39:656–662

    Article  CAS  PubMed  Google Scholar 

  • Andrews RK, López JA, Berndt MC (1997) Molecular mechanisms of platelet adhesion and activation. Int J Biochem Cell Biol 29:91–105

    Article  CAS  PubMed  Google Scholar 

  • Andrews RK, Munday AD, Mitchell CA, Berndt MC (2001) Interaction of calmodulin with the cytoplasmic domain of the glycoprotein Ib-IX-V complex. Blood 98:681–687

    Article  CAS  PubMed  Google Scholar 

  • Andrews RK, Suzuki-Inoue K, Shen Y, Tulasne D, Watson SP, Berndt MC (2002) Interaction of calmodulin with the cytoplasmic domain of platelet glycoprotein VI. Blood 99:4219–4221

    Article  CAS  PubMed  Google Scholar 

  • Andrews RK, Du X, Berndt MC (2007) The 14-3-3ζ–GPIb-IX-V complex as an antiplatelet target. Drug News Perspect 20:285–292

    Article  CAS  PubMed  Google Scholar 

  • Andrews RK, Arthur JA, Gardiner EE (2014a) Neutrophil extracellular traps (NETs) and the role of platelets in infection. Thromb Haemostas 112(4):659–665

    Article  Google Scholar 

  • Andrews RK, Arthur JF, Gardiner EE (2014b) Targeting GPVI as a novel antithrombotic strategy. J Blood Med 5:59–68

    CAS  PubMed  PubMed Central  Google Scholar 

  • Armstrong PC, Kirkby NS, Chan MV, Finsterbusch M, Hogg N, Nourshargh S, Warner TD (2015) Novel whole blood assay for phenotyping platelet reactivity in mice identifies ICAM-1 as a mediator of platelet-monocyte interaction. Blood 126(10):e11–e18

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Arthur JF, Matzaris M, Gardiner EE, Taylor SG, Wijeyewickrema LC, Ozaki Y, Kahn ML, Andrews RK, Berndt MC (2005) Glycoprotein (GP)VI is associated with GPIb-IX-V on the membrane of resting and activated platelets. Thromb Haemostas 93:716–723

    CAS  Google Scholar 

  • Arthur JF, Dunkley S, Andrews RK (2007) Platelet glycoprotein VI-related clinical defects. Br J Haematol 139:363–372

    Article  CAS  PubMed  Google Scholar 

  • Astarita JL, Acton SE, Turley SJ (2012) Podoplanin: emerging functions in development, the immune system, and cancer. Front Immunol 3:283

    Article  PubMed  PubMed Central  Google Scholar 

  • Benard SA, Smith TM, Cunningham K, Jacob J, DeSilva T, Lin L, Shaw GD, Kriz R, Kelleher KS (2008) Identification of peptide antagonists to glycoprotein Ibα that selectively inhibit von Willebrand factor dependent platelet aggregation. Biochemistry 47(16):4674–4682

    Article  CAS  PubMed  Google Scholar 

  • Bender M, Hagedorn I, Nieswandt B (2011) Genetic and antibody-induced glycoprotein VI deficiency equally protects mice from mechanically and FeCl3-induced thrombosis. J Thromb Haemost 9(7):1423–1426

    Article  CAS  PubMed  Google Scholar 

  • Bender M, May F, Lorenz V, Thielmann I, Hagedorn I, Finney BA, Vögtle T, Remer K, Braun A, Bösl M, Watson SP, Nieswandt B (2013) Combined in vivo depletion of glycoprotein VI and C-type lectin-like receptor 2 severely compromises hemostasis and abrogates arterial thrombosis in mice. Arterioscler Thromb Vasc Biol 33:926–934

    Article  CAS  PubMed  Google Scholar 

  • Bénézech C, Nayar S, Finney BA, Withers DR, Lowe K, Desanti GE, Marriott CL, Watson SP, Caamaño JH, Buckley CD, Barone F (2014) CLEC-2 is required for development and maintenance of lymph nodes. Blood 123(20):3200–3207

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Bergmeier W, Piffath CL, Goerge T, Cifuni SM, Ruggeri ZM, Ware J, Wagner DD (2006) The role of platelet adhesion receptor GPIbα far exceeds that of its main ligand, von Willebrand factor, in arterial thrombosis. Proc Natl Acad Sci U S A 103:16900–16905

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Berndt MC, Phillips DR (1981) Purification and preliminary physicochemical characterization of human platelet membrane glycoprotein V. J Biol Chem 256(1):59–65

    CAS  PubMed  Google Scholar 

  • Best D, Senis YA, Jarvis GE, Eagleton HJ, Roberts DJ, Saito T, Jung SM, Moroi M, Harrison P, Green FR, Watson SP (2003) GPVI levels in platelets: relationship to platelet function at high shear. Blood 102(8):2811–2818

    Article  CAS  PubMed  Google Scholar 

  • Bombeli T, Schwartz BR, Harlan JM (1998) Adhesion of activated platelets to endothelial cells: evidence for a GPIIbIIIa-dependent bridging mechanism and novel roles for endothelial intercellular adhesion molecule 1 (ICAM-1), αvβ3 integrin, and GPIbα. J Exp Med 187(3):329–339

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Brinkmann V, Reichard U, Goosmann C, Fauler B, Uhlemann Y, Weiss DS, Weinrauch Y, Zychlinsky A (2004) Neutrophil extracellular traps kill bacteria. Science 303(5663):1532–1535

    Article  CAS  PubMed  Google Scholar 

  • Bryckaert M, Rosa JP, Denis CV, Lenting PJ (2015) Of von Willebrand factor and platelets. Cell Mol Life Sci 72(2):307–326

    Article  CAS  PubMed  Google Scholar 

  • Bültmann A, Li Z, Wagner S, Peluso M, Schönberger T, Weis C, Konrad I, Stellos K, Massberg S, Nieswandt B, Gawaz M, Ungerer M, Münch G (2010) Impact of glycoprotein VI and platelet adhesion on atherosclerosis—a possible role of fibronectin. J Mol Cell Cardiol 49(3):532–542

    Article  PubMed  CAS  Google Scholar 

  • Burkhart JM, Vaudel M, Gambaryan S, Radau S, Walter U, Martens L, Geiger J, Sickmann A, Zahedi RP (2012) The first comprehensive and quantitative analysis of human platelet protein composition allows the comparative analysis of structural and functional pathways. Blood 120(15):e73–e82

    Article  CAS  PubMed  Google Scholar 

  • Cheli Y, Jensen D, Marchese P, Habart D, Wiltshire T, Cooke M, Fernandez JA, Ware J, Ruggeri ZM, Kunicki TJ (2008) The Modifier of hemostasis (Mh) locus on chromosome 4 controls in vivo hemostasis of GP6-/- mice. Blood 111(3):1266–1273

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Chen H, Locke D, Liu Y, Liu C, Kahn ML (2002) The platelet receptor GPVI mediates both adhesion and signalling responses to collagen in a receptor density-dependent fashion. J Biol Chem 277(4):3011–3019

    Article  CAS  PubMed  Google Scholar 

  • Chung DW (2015) Polymerized fibrin activates glycoprotein VI. Blood 126(5):570–571

    Article  CAS  PubMed  Google Scholar 

  • Coller BS (2015) αIIbβ3: structure and function. J Thromb Haemost 13(Suppl 1):S17–S25

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Cosemans JM, Angelillo-Scherrer A, Mattheij NJ, Heemskerk JW (2013) The effects of arterial flow on platelet activation, thrombus growth, and stabilization. Cardiovasc Res 99:342–352

    Article  CAS  PubMed  Google Scholar 

  • Cox D, Kerrigan SW, Watson SP (2011) Platelets and the innate immune system: mechanisms of bacterial-induced platelet activation. J Thromb Haemost 9(6):1097–1107

    Article  CAS  PubMed  Google Scholar 

  • Cranmer SL, Ashworth KJ, Yao Y, Berndt MC, Ruggeri ZM, Andrews RK, Jackson SP (2011) High shear-dependent loss of membrane integrity and defective platelet adhesion following disruption of the GPIbα-filamin interaction. Blood 117(9):2718–2727

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Da Q, Teruya M, Guchhait P, Teruya J, Olson JS, Cruz MA (2015) Free hemoglobin increases von Willebrand factor-mediated platelet adhesion in vitro: implications on circulatory devices. Blood 126(20):2338–2341

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • De Candia E (2012) Mechanisms of platelet activation by thrombin: a short history. Thromb Res 129(3):250–256

    Article  PubMed  CAS  Google Scholar 

  • Deckmyn H, De Meyer SF, Broos K, Vanhoorelbeke K (2012) Inhibitors of interactions between collagen and its receptors on platelets. Antiplatelet Agents (210):311–328

    Google Scholar 

  • Ed Rainger G, Chimen M, Harrison MJ, Yates CM, Harrison P, Watson SP, Lordkipanidzé M, Nash GB (2015) The role of platelets in the recruitment of leukocytes during vascular disease. Platelets 26(6):507–520

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Estevez B, Shen B, Du X (2015) Targeting integrin and integrin signalling in treating thrombosis. Arterioscler Thromb Vasc Biol 35:24–29

    Article  CAS  PubMed  Google Scholar 

  • Etulain J, Martinod K, Wong SL, Cifuni SM, Schattner M, Wagner DD (2015) P-selectin promotes neutrophil extracellular trap formation in mice. Blood 126(2):242–246

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Farndale RW, Slatter DA, Siljander PR, Jarvis GE (2007) Platelet receptor recognition and cross-talk in collagen-induced activation of platelets. J Thromb Haemost 5(Suppl 1):220–229

    Article  CAS  PubMed  Google Scholar 

  • Feys HB, Roodt J, Vandeputte N, Pareyn I, Mottl H, Hou S, Lamprecht S, Van Rensburg WJ, Deckmyn H, Vanhoorelbeke K (2012) Inhibition of von Willebrand factor-platelet glycoprotein Ib interaction prevents and reverses symptoms of acute acquired thrombotic thrombocytopenic purpura in baboons. Blood 120(17):3611–3614

    Article  CAS  PubMed  Google Scholar 

  • Fong KP, Barry C, Tran AN, Traxler EA, Wannemacher KM, Tang HY, Speicher KD, Blair IA, Speicher DW, Grosser T, Brass LF (2011) Deciphering the human platelet sheddome. Blood 117(1):e15–e26

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Franco AT, Corken A, Ware J (2015) Platelets at the interface of thrombosis, inflammation, and cancer. Blood 126(5):582–588

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gachet C (2015) Antiplatelet drugs: which targets for which treatments? J Thromb Haemost 13(Suppl 1):S313–S322

    Article  CAS  PubMed  Google Scholar 

  • García A, Zitzmann N, Watson SP (2004a) Analyzing the platelet proteome. Semin Thromb Hemost 30(4):485–489

    Article  PubMed  Google Scholar 

  • García A, Prabhakar S, Brock CJ, Pearce AC, Dwek RA, Watson SP, Hebestreit HF, Zitzmann N (2004b) Extensive analysis of the human platelet proteome by two-dimensional gel electrophoresis and mass spectrometry. Proteomics 4(3):656–668

    Article  PubMed  CAS  Google Scholar 

  • Gardiner EE, Andrews RK (2014a) Structure and function of platelet receptors initiating blood clotting. In: Kimmel M, Corey S (eds) A systems biology approach to hematology. Springer, New York, pp 263–275

    Google Scholar 

  • Gardiner EE, Andrews RK (2014b) Platelet receptor expression and shedding: GPIb-IX-V and GPVI. Transfus Med Rev 28(2):56–60

    Article  PubMed  Google Scholar 

  • Gardiner EE, Andrews RK (2014c) Plasma sGPVI: changing levels in human disease. Thromb Res 133:306–307

    Article  CAS  PubMed  Google Scholar 

  • Gardiner EE, Arthur JF, Kahn ML, Berndt MC, Andrews RK (2004) Regulation of platelet membrane levels of glycoprotein VI by a platelet-derived metalloproteinase. Blood 104:3611–3617

    Article  CAS  PubMed  Google Scholar 

  • Gardiner EE, Karunakaran D, Shen Y, Andrews RK, Berndt MC (2007) Controlled shedding of platelet glycoprotein (GP)VI and GPIb-IX-V by ADAM family metalloproteinases. J Thromb Haemost 5:1530–1537

    Article  CAS  PubMed  Google Scholar 

  • Gardiner EE, Arthur JF, Shen Y, Karunakaran D, Moore LA, Schulte Am Esch II Jr, Andrews RK, Berndt MC (2010) GPIbα-selective activation of platelets induces platelet signalling events comparable to GPVI activation events. Platelets 21:244–252

    Article  CAS  PubMed  Google Scholar 

  • Gardiner EE, Ward CM, Andrews RK (2012) The NET effect of clot formation. J Thromb Haemost 10(1):133–135

    Article  CAS  PubMed  Google Scholar 

  • Gawaz M, Vogel S, Pfannenberg C, Pichler B, Langer H, Bigalke B (2014) Implications of glycoprotein VI for theranostics. Thromb Haemost 112(1):26–31

    Article  CAS  PubMed  Google Scholar 

  • Gitz E, Koopman CD, Giannas A, Koekman CA, van den Heuvel DJ, Deckmyn H, Akkerman JW, Gerritsen HC, Urbanus RT (2013) Platelet interaction with von Willebrand factor is enhanced by shear-induced clustering of glycoprotein Ibα. Haematologica 98(11):1810–1818

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Glezeva N, Gilmer JF, Watson CJ, Ledwidge M (2016) A central role for monocyte-platelet interactions in heart failure. J Cardiovasc Pharmacol Ther 21:245–261

    Article  PubMed  Google Scholar 

  • Grässle S, Huck V, Pappelbaum KI, Gorzelanny C, Aponte-Santamaría C, Baldauf C, Gräter F, Schneppenheim R, Obser T, Schneider SW (2014) von Willebrand factor directly interacts with DNA from neutrophil extracellular traps. Arterioscler Thromb Vasc Biol 34(7):1382–1389

    Article  PubMed  CAS  Google Scholar 

  • Grewal PK (2010) The Ashwell-Morell receptor. Methods Enzymol 479:223–241

    Article  CAS  PubMed  Google Scholar 

  • Grewal PK, Uchiyama S, Ditto D, Varki N, Le DT, Nizet V, Marth JD (2008) The Ashwell receptor mitigates the lethal coagulopathy of sepsis. Nat Med 14(6):648–655

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Herzog BH, Fu J, Wilson SJ, Hess PR, Sen A, McDaniel JM, Pan Y, Sheng M, Yago T, Silasi-Mansat R, McGee S, May F, Nieswandt B, Morris AJ, Lupu F, Coughlin SR, McEver RP, Chen H, Kahn ML, Xia L (2013) Podoplanin maintains high endothelial venule integrity by interacting with platelet CLEC-2. Nature 502(7469):105–109

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Inoue O, Suzuki-Inoue K, McCarty OJ, Moroi M, Ruggeri ZM, Kunicki TJ, Ozaki Y, Watson SP (2006) Laminin stimulates spreading of platelets through integrin alpha6beta1-dependent activation of GPVI. Blood 107(4):1405–1412

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jenne CN, Urrutia R, Kubes P (2013) Platelets: bridging hemostasis, inflammation, and immunity. Int J Lab Hematol 35:254–261

    Article  CAS  PubMed  Google Scholar 

  • Jiang P, Jandrot-Perrus M (2014) New advances in treating thrombotic diseases: GPVI as a platelet drug target. Drug Discov Today 19(9):1471–1475

    Article  CAS  PubMed  Google Scholar 

  • Jiang P, Loyau S, Tchitchinadze M, Ropers J, Jondeau G, Jandrot-Perrus M (2015) Inhibition of glycoprotein VI clustering by collagen as a mechanism of inhibiting collagen-induced platelet responses: the example of losartan. PLoS One 10(6):e0128744

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Kroll MH, Hellums JD, McIntire LV, Schafer AI, Moake JL (1996) Platelets and shear stress. Blood 88(5):1525–1541

    CAS  PubMed  Google Scholar 

  • Kruss S, Erpenbeck L, Amschler K, Mundinger TA, Helms H-J, Friede T, Andrews RK, Schön MP, Spatz JP (2013) Adhesion maturation of neutrophils on nanoscopically presented platelet receptor GPIbα. ACS Nano 7(11):9984–9996

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lancellotti S, Dragani A, Ranalli P, Petrucci G, Basso M, Tartaglione R, Rocca B, De Cristofaro R (2015) Qualitative and quantitative modifications of von Willebrand factor in patients with essential thrombocythemia and controlled platelet count. J Thromb Haemost 13(7):1226–1237

    Article  CAS  PubMed  Google Scholar 

  • Li N (2015) Platelets in cancer metastasis: to help the “villain” to do evil. Int J Cancer 138(9):2078–2087

    Article  PubMed  CAS  Google Scholar 

  • Li J, van der Wal DE, Zhu G, Xu M, Yougbare I, Ma L, Vadasz B, Carrim N, Grozovsky R, Ruan M, Zhu L, Zeng Q, Tao L, Zhai ZM, Peng J, Hou M, Leytin V, Freedman J, Hoffmeister KM, Ni H (2015) Desialylation is a mechanism of Fc-independent platelet clearance and a therapeutic target in immune thrombocytopenia. Nat Commun 6:7737

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Libby P (2015) Murine “model” monotheism: an iconoclast at the altar of mouse. Circ Res 117(11):921–925

    Article  CAS  PubMed  Google Scholar 

  • Litvinov RI, Weisel JW (2015) Not fibrin(ogen), but fibrinogen or fibrin. Blood 126(17):1977–1978

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • López JA, Dong JF (2005) Shear stress and the role of high molecular weight von Willebrand factor multimers in thrombus formation. Blood Coagul Fibrinolysis 16(Suppl 1):S11–S16

    Article  PubMed  Google Scholar 

  • López JA, Andrews RK, Afshar-Kharghan V, Berndt MC (1998) Bernard-Soulier syndrome. Blood 91:4397–4418

    PubMed  Google Scholar 

  • Loyau S, Dumont B, Ollivier V, Boulaftali Y, Feldman L, Ajzenberg N, Jandrot-Perrus M (2012) Platelet glycoprotein VI dimerization, an active process inducing receptor competence, is an indicator of platelet reactivity. Arterioscler Thromb Vasc Biol 32(3):778–785

    Article  CAS  PubMed  Google Scholar 

  • Mammadova-Bach E, Ollivier V, Loyau S, Schaff M, Dumont B, Favier R, Freyburger G, Latger-Cannard V, Nieswandt B, Gachet C, Mangin PH, Jandrot-Perrus M (2015) Platelet glycoprotein VI binds to polymerized fibrin and promotes thrombin generation. Blood 126(5):683–691

    Article  CAS  PubMed  Google Scholar 

  • Martinod K, Wagner DD (2014) Thrombosis: tangled up in NETs. Blood 123(18):2768–2776

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Maurer E, Schaff M, Receveur N, Bourdon C, Mercier L, Nieswandt B, Dubois C, Jandrot-Perrus M, Goetz J, Lanza F, Gachet C, Mangin PH (2015) Fibrillar cellular fibronectin supports efficient platelet function and procoagulant activity. Thromb Haemost 114(6):1175–1188

    Article  PubMed  Google Scholar 

  • McEwan PA, Andrews RK, Emsley J (2009) Glycoprotein Ibα inhibitor complex structure reveals a combined steric and allosteric mechanism of von Willebrand factor antagonism. Blood 114:4883–4885

    Article  CAS  PubMed  Google Scholar 

  • Metharom P, Berndt MC, Baker R, Andrews RK (2015) Current state of antiplatelet therapy. Arterioscler Thromb Vasc Biol 35(6):1327–1338

    Article  CAS  PubMed  Google Scholar 

  • Meyer Dos Santos S, Blankenbach K, Scholich K, Dörr A, Monsefi N, Keese M, Linke B, Deckmyn H, Nelson K, Harder S (2015) Platelets from flowing blood attach to the inflammatory chemokine CXCL16 expressed in the endothelium of the human vessel wall. Thromb Haemost 114(2):297–312

    Article  CAS  PubMed  Google Scholar 

  • Moog S, Mangin P, Lenain N, Strassel C, Ravanat C, Schuhler S, Freund M, Santer M, Kahn M (2001) Platelet glycoprotein V binds to collagen and participates in platelet adhesion and aggregation. Blood 98(4):1038–1046

    Article  CAS  PubMed  Google Scholar 

  • Morrell CN, Aggrey AA, Chapman LM, Modjeski KL (2014) Emerging roles for platelets as immune and inflammatory cells. Blood 123(18):2759–2767

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Mu F-T, Andrews RK, Arthur JF, Munday AM, Cranmer SL, Jackson SP, Stomski F, Lopez AF, Berndt MC (2008) A functional 14-3-3ζ-independent association of PI3-kinase with glycoprotein Ibα, the major ligand-binding subunit of the platelet glycoprotein Ib-IX-V complex. Blood 111:4580–4587

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Mu F-T, Cranmer SL, Andrews RK, Berndt MC (2010) Functional association of PI3-kinase with platelet glycoprotein Ibα, the major ligand-binding subunit of the glycoprotein Ib-IX-V complex. J Thromb Haemost 8:324–330

    Article  CAS  PubMed  Google Scholar 

  • Murphy D, Aubron C, Lohito P, Gardiner EE, Andrews RK, Davis AK (2015) Extracorporeal membrane oxygenation (ECMO)—haemostatic complications. Transf Med Rev 29(2):90–101

    Article  Google Scholar 

  • Navarro-Núñez L, Langan SA, Nash GB, Watson SP (2013) The physiological and pathophysiological roles of platelet CLEC-2. Thromb Haemost 109(6):991–998

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Navarro-Núñez L, Pollitt AY, Lowe K, Latif A, Nash GB, Watson SP (2015) Platelet adhesion to podoplanin under flow is mediated by the receptor CLEC-2 and stabilised by Src/Syk-dependent platelet signalling. Thromb Haemost 113(5):1109–1120

    Article  PubMed  PubMed Central  Google Scholar 

  • Nuyttens BP, Thijs T, Deckmyn H, Broos K (2011) Platelet adhesion to collagen. Thromb Res 127:S26–S29

    Article  CAS  PubMed  Google Scholar 

  • Owaynat H, Yermolenko IS, Turaga R, Lishko VK, Sheller MR, Ugarova TP (2015) Deposition of fibrinogen on the surface of in vitro thrombi prevents platelet adhesion. Thromb Res 136(6):1231–1239

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ozaki Y, Suzuki-Inoue K, Inoue O (2013) Platelet receptors activated via multimerization: glycoprotein VI, GPIb-IX-V, and CLEC-2. J Thromb Haemost 11(Suppl 1):330–339

    Article  PubMed  Google Scholar 

  • Padilla A, Moake JL, Bernardo A, Ball C, Wang Y, Arya M, Nolasco L, Turner N, Berndt MC, Anvari B, López JA, Dong JF (2004) P-selectin anchors newly released ultralarge von Willebrand factor multimers to the endothelial cell surface. Blood 103(6):2150–2156

    Article  CAS  PubMed  Google Scholar 

  • Pawelski H, Lang D, Reuter S (2014) Interactions of monocytes and platelets: implication for life. Front Biosci (Schol Ed) 6:75–91

    Google Scholar 

  • Penz S, Reininger AJ, Brandl R, Goyal P, Rabie T, Bernlochner I, Rother E, Goetz C, Engelmann B, Smethurst PA, Ouwehand WH, Farndale R, Nieswandt B, Siess W (2005) Human atheromatous plaques stimulate thrombus formation by activating platelet glycoprotein VI. FASEB J 19(8):898–909

    Article  CAS  PubMed  Google Scholar 

  • Prasad JM, Gorkun OV, Raghu H, Thornton S, Mullins ES, Palumbo JS, Ko YP, Höök M, David T, Coughlin SR, Degen JL, Flick MJ (2015) Mice expressing a mutant form of fibrinogen that cannot support fibrin formation exhibit compromised antimicrobial host defense. Blood 126(17):2047–2058

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Qiao J, Shen Y, Gardiner EE, Andrews RK (2010) Proteolysis of platelet receptors in humans and other species. Biol Chem 391:893–900

    Article  CAS  PubMed  Google Scholar 

  • Romo GM, Dong JF, Schade AJ, Gardiner EE, Kansas GS, Li CQ, McIntire LV, Berndt MC, López JA (1999) The glycoprotein Ib-IX-V complex is a platelet counterreceptor for P-selectin. J Exp Med 190(6):803–814

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rondina MT, Weyrich AS, Zimmerman GA (2013) Platelets as cellular effectors of inflammation in vascular diseases. Circ Res 112:1506–1519

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Safiullin R, Christenson W, Owaynat H, Yermolenko IS, Kadirov MK, Ros R, Ugarova TP (2015) Fibrinogen matrix deposited on the surface of biomaterials acts as a natural anti-adhesive coating. Biomaterials 67:151–159

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Salles II, Crawley JT (2012) Blocking VWF platelet binding to treat TTP. Blood 120(17):3390–3392

    Article  CAS  PubMed  Google Scholar 

  • Samaha FF, Hibbard C, Sacks J, Chen H, Varello MA, George T, Kahn ML (2005) Density of platelet collagen receptors glycoprotein VI and α2β1 and prior myocardial infarction in human subjects, a pilot study. Med Sci Monitor 11(5):CR224–CR229

    CAS  Google Scholar 

  • Schrottmaier WC, Kral JB, Badrnya S, Assinger A (2015) Aspirin and P2Y12 Inhibitors in platelet-mediated activation of neutrophils and monocytes. Thromb Haemost 114(3):478–489

    Article  PubMed  Google Scholar 

  • Seizer P, Gawaz M, May AE (2008) Platelet-monocyte interactions—a dangerous liaison linking thrombosis, inflammation and atherosclerosis. Curr Med Chem 15(20):1976–1980

    Article  CAS  PubMed  Google Scholar 

  • Senzel L, Gnatenko DV, Bahou WF (2009) The platelet proteome. Curr Opin Hematol 16(5):329–333

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Shao L, Wu Y, Zhou H, Qin P, Ni H, Peng J, Hou M (2015) Successful treatment with oseltamivir phosphate in a patient with chronic immune thrombocytopenia positive for anti-GPIb/IX autoantibody. Platelets 26(5):495–497

    Article  CAS  PubMed  Google Scholar 

  • Shen Y, Romo GM, Dong J-F, Schade A, McIntire LV, Kenny D, Whisstock JC, Berndt MC, López JA, Andrews RK (2000) Requirement of leucine-rich repeats of GPIbα for shear-dependent and static binding of von Willebrand factor to the platelet membrane GP Ib-IX-V complex. Blood 95:903–910

    CAS  PubMed  Google Scholar 

  • Shen Y, Cranmer SL, Aprico A, Whisstock JC, Jackson SP, Berndt MC, Andrews RK (2006) Leucine-rich repeats 2-4 (Leu60-Glu128) of platelet glycoprotein Ibα regulate shear-dependent cell adhesion to von Willebrand factor. J Biol Chem 281:26419–26423

    Article  CAS  PubMed  Google Scholar 

  • Simon DI (2012) Inflammation and vascular injury: basic discovery to drug development. Circ J 76(8):1811–1818

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Simon SI, Goldsmith HL (2002) Leukocyte adhesion dynamics in shear flow. Ann Biomed Eng 30(3):315–332

    Article  PubMed  Google Scholar 

  • Simon DI, Chen Z, Xu H, Li CQ, Dong JF, McIntire LV, Ballantyne CM, Zhang L, Furman MI, Berndt MC, López JA (2000) Platelet glycoprotein Ibα is a counterreceptor for the leukocyte integrin Mac-1 (CD11b/CD18). J Exp Med 192(2):193–204

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Suzuki-Inoue K (2011) Essential in vivo roles of the platelet activation receptor CLEC-2 in tumour metastasis, lymphangiogenesis and thrombus formation. J Biochem 150(2):127–132

    Article  CAS  PubMed  Google Scholar 

  • Suzuki-Inoue K, Tulasne D, Bori-Sanz T, Inoue O, Jung SM, Moroi M, Shen Y, Andrews RK, Berndt MC, Watson SP (2002) Association of fyn and lyn with the proline rich domain of GPVI regulates intracellular signalling. J Biol Chem 277:21561–21566

    Article  CAS  PubMed  Google Scholar 

  • Ungerer M, Rosport K, Bultmann A, Piechatzek R, Uhland K, Schlieper P, Gawaz M, Munch G (2011) Novel antiplatelet drug revacept (Dimeric Glycoprotein VI-Fc) specifically and efficiently inhibited collagen-induced platelet aggregation without affecting general hemostasis in humans. Circulation 123:1891–1899

    Article  CAS  PubMed  Google Scholar 

  • Varga-Szabo D, Pleines I, Nieswandt B (2008) Cell adhesion mechanisms in platelets. Arterioscler Thromb Vasc Biol 28:1–10

    Article  CAS  Google Scholar 

  • Ward CM, Tetaz TJ, Andrews RK, Berndt MC (1997) Binding of the von Willebrand factor A1 domain to histone. Thromb Res 86:469–477

    Article  CAS  PubMed  Google Scholar 

  • Watson SP (2009) Platelet activation by extracellular matrix proteins in haemostasis and thrombosis. Curr Pharm Des 15(12):1358–1372

    Article  CAS  PubMed  Google Scholar 

  • Watson SP, Bahou WF, Fitzgerald D, Ouwehand W, Rao AK, Leavitt AD, ISTH Platelet Physiology Subcommittee (2005) Mapping the platelet proteome: a report of the ISTH Platelet Physiology Subcommittee. J Thromb Haemost 3(9):2098–2101

    Article  CAS  PubMed  Google Scholar 

  • Yago T, Lou J, Wu T, Yang J, Miner JJ, Coburn L, López JA, Cruz MA, Dong JF, McIntire LV, McEver RP, Zhu C (2008) Platelet glycoprotein Ibα forms catch bonds with human WT vWF but not with type 2B von Willebrand disease vWF. J Clin Invest 118(9):3195–3207

    CAS  PubMed  PubMed Central  Google Scholar 

  • Yong AS, Pennings GJ, Chang M, Hamzah A, Chung T, Qi M, Brieger D, Behnia M, Krilis SA, Ng MK, Lowe HC, Kritharides L (2011) Intracoronary shear-related up-regulation of platelet P-selectin and platelet-monocyte aggregation despite the use of aspirin and clopidogrel. Blood 117(1):11–20

    Article  CAS  PubMed  Google Scholar 

  • Zahid M, Mangin P, Loyau S, Hechler B, Billiald P, Gachet C, Jandrot-Perrus M (2012) The future of glycoprotein VI as an antithrombotic target. J Thromb Haemost 10(12):2418–2427

    Article  CAS  PubMed  Google Scholar 

  • Zheng XL (2015) ADAMTS13 and von Willebrand factor in thrombotic thrombocytopenic purpura. Annu Rev Med 66:211–225

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhou Z, Nguyen TC, Guchhait P, Dong JF (2010) von Willebrand factor, ADAMTS-13, and thrombotic thrombocytopenic purpura. Semin Thromb Hemost 36(1):71–81

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

We thank the editors for the opportunity to provide this review and our colleagues and coworkers for many helpful discussions. We thank the National Health and Medical Research Council (NH&MRC), Australia, for financial support.

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to Elizabeth E. Gardiner or Robert K. Andrews .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer International Publishing AG

About this chapter

Cite this chapter

Gardiner, E.E., Andrews, R.K. (2017). Platelet Adhesion. In: Gresele, P., Kleiman, N., Lopez, J., Page, C. (eds) Platelets in Thrombotic and Non-Thrombotic Disorders. Springer, Cham. https://doi.org/10.1007/978-3-319-47462-5_23

Download citation

Publish with us

Policies and ethics