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Invasive Measurement of Pulmonary Function in Mice

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Acute Lung Injury and Repair

Part of the book series: Respiratory Medicine ((RM))

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Abstract

Disease-related structural changes often result in functional consequences. Invasive pulmonary function analysis has significantly advanced our knowledge of lung diseases. The flexiVent in vivo invasive system offers families of dedicated analyses that allow the characterization of respiratory mechanics for laboratory studies of lung disease models in animals. The common parameters derived from these analyses have been shown in animals to change characteristically following disease model establishment and disease intervention that reflect human pathologies such as asthma, pulmonary fibrosis, and acute lung injury.

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References

  1. Allen GB, Cloutier ME, Larrabee YC, Tetenev K, Smiley ST, Bates JH. Neither fibrin nor plasminogen activator inhibitor-1 deficiency protects lung function in a mouse model of acute lung injury. Am J Physiol Lung Cell Mol Physiol. 2009;296(3):L277–85.

    Article  CAS  PubMed  Google Scholar 

  2. Baron RM, Choi AJ, Owen CA, Choi AM. Genetically manipulated mouse models of lung disease: potential and pitfalls. Am J Physiol Lung Cell Mol Physiol. 2012;302(6):L485–97.

    Article  CAS  PubMed  Google Scholar 

  3. Bates J, Irvin C, Brusasco V, Drazen J, Fredberg J, Loring S, Eidelman D, Ludwig M, Macklem P, Martin J, Milic-Emili J, Hantos Z, Hyatt R, Lai-Fook S, Leff A, Solway J, Lutchen K, Suki B, Mitzner W, Paré P, Pride N, Sly P. The use and misuse of Penh in animal models of lung disease. Am J Respir Cell Mol Biol. 2004;31(3):373–4.

    Article  CAS  PubMed  Google Scholar 

  4. Caceres AI, Brackmann M, Elia MD, Bessac BF, del Camino D, D’Amours M, Witek JS, Fanger CM, Chong JA, Hayward NJ, Homer RJ, Cohn L, Huang X, Moran MM, Jordt SE. A sensory neuronal ion channel essential for airway inflammation and hyperreactivity in asthma. Proc Natl Acad Sci U.S.A. 2009;106(22):9099–104.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Card JW, Voltz JW, Carey MA, Bradbury JA, Degraff LM, Lih FB, Bonner JC, Morgan DL, Flake GP, Zeldin DC. Cyclooxygenase-2 deficiency exacerbates bleomycin-induced lung dysfunction but not fibrosis. Am J Respir Cell Mol Biol. 2007;37(3):300–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Cojocaru A, Irvin CG, Haverkamp HC, Bates JH. Computational assessment of airway wall stiffness in vivo in allergically inflamed mouse models of asthma. J Appl Physiol. (1985). 2008;104(6):1601–1610.

    Google Scholar 

  7. Cozzi E, Ackerman KG, Lundequist A, Drazen JM, Boyce JA, Beier DR. The naive airway hyperresponsiveness of the A/J mouse is Kit-mediated. Proc Natl Acad Sci USA. 2011;108(31):12787–92.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. De Sanctis GT, Merchant M, Beier DR, Dredge RD, Grobholz JK, Martin TR, Lander ES, Drazen JM. Quantitative locus analysis of airway hyperresponsiveness in A/J and C57BL/6 J mice. Nat Genet. 1995;11(2):150–4.

    Article  PubMed  Google Scholar 

  9. Drazen JM, Finn PW, De Sanctis GT. Mouse models of airway responsiveness: physiological basis of observed outcomes and analysis of selected examples using these outcome indicators. Annu Rev Physiol. 1999;61:593–625. Review.

    Google Scholar 

  10. Duguet A, Biyah K, Minshall E, Gomes R, Wang C-G, Taoudi-Benchekroun M, Bates JHT, Eidelman DH. Bronchial Responsiveness among Inbred Mouse Strains. Role of Airway Smooth-Muscle Shortening Velocity. Am J Respir Crit Care Med. 2000;161(3):839–48.

    Article  CAS  PubMed  Google Scholar 

  11. Egger C, Gérard C, Vidotto N, Accart N, Cannet C, Dunbar A, Tigani B, Piaia A, Jarai G, Jarman E, Schmid HA, Beckmann N. Lung volume quantified by MRI reflects extracellular-matrix deposition and altered pulmonary function in bleomycin models of fibrosis: effects of SOM230. Am J Physiol Lung Cell Mol Physiol. 2014;306(12):L1064–77.

    Article  CAS  PubMed  Google Scholar 

  12. Ferreira TP, de Arantes AC, do Nascimento CV, Olsen PC, Trentin PG, Rocco PR, Hogaboam CM, Puri RK, Martins MA, Silva PM. IL-13 immunotoxin accelerates resolution of lung pathological changes triggered by silica particles in mice. J Immunol. 2013;191(10):5220–5229.

    Google Scholar 

  13. Glaab T, Taube C, Braun A, Mitzner W. Invasive and noninvasive methods for studying pulmonary function in mice. Respir Res. 2007;8:63. Review.

    Google Scholar 

  14. Grünig G, Warnock M, Wakil AE, Venkayya R, Brombacher F, Rennick DM, Sheppard D, Mohrs M, Donaldson DD, Locksley RM, Corry DB. Requirement for IL-13 independently of IL-4 in experimental asthma. Science. 1998;282(5397):2261–3.

    Article  PubMed  PubMed Central  Google Scholar 

  15. Håkansson HF, Smailagic A, Brunmark C, Miller-Larsson A, Lal H. Altered lung function relates to inflammation in an acute LPS mouse model. Pulm Pharmacol Ther. 2012;25(5):399–406.

    Article  CAS  PubMed  Google Scholar 

  16. Hamelmann E, Schwarze J, Takeda K, Oshiba A, Larsen GL, Irvin CG, Gelfand EW. Noninvasive measurement of airway responsiveness in allergic mice using barometric plethysmography. Am J Respir Crit Care Med. 1997;156(3 Pt 1):766–75.

    Article  CAS  PubMed  Google Scholar 

  17. Hantos Z, Daroczy B, Suki B, Nagy S, Fredberg JJ. Input impedance and peripheral inhomogeneity in dog lungs. J Appl Physiol. 1992;72(1):168–78.

    Article  CAS  PubMed  Google Scholar 

  18. Jucker M. The benefits and limitations of animal models for translational research in neurodegenerative diseases. Nat Med. 2010;16(11):1210–4.

    Article  CAS  PubMed  Google Scholar 

  19. Kearley J, Erjefalt JS, Andersson C, Benjamin E, Jones CP, Robichaud A, Pegorier S, Brewah Y, Burwell TJ, Bjermer L, Kiener PA, Kolbeck R, Lloyd CM, Coyle AJ, Humbles AA. IL-9 governs allergen-induced mast cell numbers in the lung and chronic remodeling of the airways. Am J Respir Crit Care Med. 2011;183(7):865–75.

    Article  CAS  PubMed  Google Scholar 

  20. Köhl J, Baelder R, Lewkowich IP, Pandey MK, Hawlisch H, Wang L, Best J, Herman NS, Sproles AA, Zwirner J, Whitsett JA, Gerard C, Sfyroera G, Lambris JD, Wills-Karp M. A regulatory role for the C5a anaphylatoxin in type 2 immunity in asthma. J Clin Invest. 2006;116(3):783–96.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Kudo M, Melton AC, Chen C, Engler MB, Huang KE, Ren X, Wang Y, Bernstein X, Li JT, Atabai K, Huang X*, Sheppard D. IL-17A produced by αβ T cells drives airway hyper-responsiveness in mice and enhances mouse and human airway smooth muscle contraction. Nat Med. 2012;18(4):547–54.

    Google Scholar 

  22. Kuperman DA, Huang XZ, Koth LL, Chang GH, Dolganov GM, Zhu Z, Elias JA, Sheppard D, Erle DJ. Direct effects of interleukin-13 on epithelial cells cause airway hyperreactivity and mucus overproduction in asthma. Nat Med. 2002;8:885–9.

    CAS  PubMed  Google Scholar 

  23. Lovgren AK, Jania LA, Hartney JM, Parsons KK, Audoly LP, Fitzgerald GA, Tilley SL, Koller BH. COX-2-derived prostacyclin protects against bleomycin-induced pulmonary fibrosis. Am J Physiol Lung Cell Mol Physiol. 2006;291(2):L144–56.

    Article  CAS  PubMed  Google Scholar 

  24. McGovern TK, Robichaud A, Fereydoonzad L, Schuessler TF, Martin JG. Evaluation of respiratory system mechanics in mice using the forced oscillation technique. J Vis Exp. 2013;75:e50172. doi:10.3791/50172.

    Google Scholar 

  25. Mouded M, Egea EE, Brown MJ, Hanlon SM, Houghton AM, Tsai LW, Ingenito EP, Shapiro SD. Epithelial cell apoptosis causes acute lung injury masquerading as emphysema. Am J Respir Cell Mol Biol. 2009;41(4):407–14.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  26. North ML, Amatullah H, Khanna N, Urch B, Grasemann H, Silverman F, Scott JA. Augmentation of arginase 1 expression by exposure to air pollution exacerbates the airways hyperresponsiveness in murine models of asthma. Respir Res. 2011;12(1):19.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Orme J Jr, Romney JS, Hopkins RO, Pope D, Chan KJ, Thomsen G, Crapo RO, Weaver LK, Pulmonary function and health-related quality of life in survivors of acute respiratory distress syndrome. Am J Respir Crit Care Med. 2003;167(5):690–694.

    Google Scholar 

  28. Peták F, Habre W, Donati YR, Hantos Z, Barazzone-Argiroffo C, Hyperoxia-induced changes in mouse lung mechanics: forced oscillations vs. barometric plethysmography. J Appl Physiol. (1985). 2001;90(6):2221–2230.

    Google Scholar 

  29. Rao S, Verkman AS. Analysis of organ physiology in transgenic mice. Am J Physiol Cell Physiol. 2000;279(1):C1–18.

    CAS  PubMed  Google Scholar 

  30. Robichaud A, Fereydoonzad L, Urovitch IB, Brunet JD. Comparative study of three flexiVent system configurations using mechanical test loads. Exp Lung Res. 2014. doi:10.3109/01902148.2014.971921 (Epub ahead of print).

  31. Shalaby KH, Gold LG, Schuessler TF, Martin JG, Robichaud A. Combined forced oscillation and forced expiration measurements in mice for the assessment of airway hyperresponsiveness. Respir Res. 2010;11:82.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Suzukawa M, Morita H, Nambu A, Arae K, Shimura E, Shibui A, Yamaguchi S, Suzukawa K, Nakanishi W, Oboki K, Kajiwara N, Ohno T, Ishii A, Körner H, Cua DJ, Suto H, Yoshimoto T, Iwakura Y, Yamasoba T, Ohta K, Sudo K, Saito H, Okumura K, Broide DH, Matsumoto K, Nakae S. Epithelial cell-derived IL-25, but not Th17 cell-derived IL-17 or IL-17F, is crucial for murine asthma. J Immunol. 2012;189(7):3641–52 Epub 2012 Aug 31.

    Article  CAS  PubMed  Google Scholar 

  33. Tanaka K-I, Azuma A, Sato K, Mizushima T. Effects of lecithinized superoxide dismutase and/or pirfenidone against bleomycin-induced pulmonary fibrosis. Chest. 2012;142(4):1011–9.

    Article  CAS  PubMed  Google Scholar 

  34. Tomioka S, Bates JH, Irvin CG. Airway and tissue mechanics in a murine model of asthma: alveolar capsule vs. forced oscillations. J Appl Physiol. (1985). 2002;93(1):263–70.

    Google Scholar 

  35. Wong PC, Cai H, Borchelt DR, Price DL. Genetically engineered mouse models of neurodegenerative diseases. Nat Neurosci. 2002;5(7):633–9.

    Article  CAS  PubMed  Google Scholar 

  36. Vanoirbeek JA, Rinaldi M, De Vooght V, Haenen S, Bobic S, Gayan-Ramirez G, Hoet PH, Verbeken E, Decramer M, Nemery B, Janssens W. Noninvasive and invasive pulmonary function in mouse models of obstructive and restrictive respiratory diseases. Am J Respir Cell Mol Biol. 2010;42(1):96–104.

    Article  CAS  PubMed  Google Scholar 

  37. Voltz JW, Card JW, Carey MA, DeGraff LM, Ferguson CD, Flake GP, Bonner JC, Korach KS, Zeldin DC. Male sex hormones exacerbate lung function impairment after bleomycin-induced pulmonary fibrosis. Am J Respir Cell Mol Biol. 2008;39(1):45–52.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Zheng T, Zhu Z, Wang Z, Homer RJ, Ma B, Riese RJ Jr, Chapman HA Jr, Shapiro SD, Elias JA. Inducible targeting of IL-13 to the adult lung causes matrix metalloproteinase- and cathepsin-dependent emphysema. J Clin Invest. 2000;106(9):1081–93.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgments

The authors thank Dr. Xin Ren for assistance in lung function measurements, Dr. Dean Sheppard for helpful comments during the preparation of this chapter.

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Authors and Affiliations

  1. Department of Medicine, University of California, San Francisco, California, USA

    Xiaozhu Huang

  2. SCIREQ Scientific Respiratory Equipment Inc., Montreal, Québec, Canada

    Annette Robichaud

Authors
  1. Xiaozhu Huang
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Correspondence to Xiaozhu Huang .

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Editors and Affiliations

  1. Pulmonary, Critical Care, Allergy and Sleep Medicine, Medical University of South Carolina, Charleston, South Carolina, USA

    Lynn M. Schnapp

  2. Division of Rheumatology and Immunology, Medical University of South Carolina, Charleston, South Carolina, USA

    Carol Feghali-Bostwick

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Huang, X., Robichaud, A. (2017). Invasive Measurement of Pulmonary Function in Mice. In: Schnapp, L., Feghali-Bostwick, C. (eds) Acute Lung Injury and Repair. Respiratory Medicine. Humana Press, Cham. https://doi.org/10.1007/978-3-319-46527-2_5

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  • DOI: https://doi.org/10.1007/978-3-319-46527-2_5

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  • Publisher Name: Humana Press, Cham

  • Print ISBN: 978-3-319-46525-8

  • Online ISBN: 978-3-319-46527-2

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