Skip to main content

Planning the Experiment

  • Chapter
  • First Online:
Elispot for Rookies (and Experts Too)

Abstract

A good Elispot experiment starts with a well-thought-through plan of execution. The quality of an experiment and its results are determined by the controls it includes. Here is a summary of important controls:

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 59.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 79.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Cox JH, Ferrari G, Janetzki S. Measurement of cytokine release at the single cell level using the ELISPOT assay. Methods. 2006;38(4):274–82.

    Article  CAS  PubMed  Google Scholar 

  2. Jahnmatz M, Kesa G, Netterlid E, Buisman AM, Thorstensson R, Ahlborg N. Optimization of a human IgG B-cell ELISpot assay for the analysis of vaccine-induced B-cell responses. J Immunol Methods. 2013;391(1-2):50–9.

    Article  CAS  PubMed  Google Scholar 

  3. Currier JR, Kuta EG, Turk E, Earhart LB, Loomis-Price L, Janetzki S, et al. A panel of MHC class I restricted viral peptides for use as a quality control for vaccine trial ELISPOT assays. J Immunol Methods. 2002;260(1-2):157–72. doi:S002217590100535X [pii].

    Article  CAS  PubMed  Google Scholar 

  4. Maecker HT, Dunn HS, Suni MA, Khatamzas E, Pitcher CJ, Bunde T, et al. Use of overlapping peptide mixtures as antigens for cytokine flow cytometry. J Immunol Methods. 2001;255(1-2):27–40. doi:S0022175901004161 [pii].

    Article  CAS  PubMed  Google Scholar 

  5. Smith JG, Joseph HR, Green T, Field JA, Wooters M, Kaufhold RM, et al. Establishing acceptance criteria for cell-mediated-immunity assays using frozen peripheral blood mononuclear cells stored under optimal and suboptimal conditions. Clin Vaccine Immunol. 2007;14(5):527–37. doi:10.1128/CVI.00435-06. CVI.00435-06 [pii].

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Bidmon N, Attig S, Rae R, Schroder H, Omokoko TA, Simon P, et al. Generation of TCR-engineered T cells and their use to control the performance of T cell assays. J Immunol. 2015;194(12):6177–89. doi:10.4049/jimmunol.1400958.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer International Publishing Switzerland

About this chapter

Cite this chapter

Janetzki, S. (2016). Planning the Experiment. In: Elispot for Rookies (and Experts Too). Techniques in Life Science and Biomedicine for the Non-Expert. Springer, Cham. https://doi.org/10.1007/978-3-319-45295-1_5

Download citation

Publish with us

Policies and ethics