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Microtechnology for Cell Manipulation and Sorting pp 1–14Cite as

Microfluidic Cell Sorting and Separation Technology

Part of the Microsystems and Nanosystems book series (MICRONANO)

Abstract

Cell sorting and separation is widely used as a critical first step for research and clinical applications where it is needed to isolate individual cell types from a heterogeneous biological sample. In this introductory chapter, we review conventional cell sorting and separation techniques and their applications. To meet the complex and diversifying needs for cell sorting, many microfluidic techniques based on diverse sorting criteria have been developed recently. Microfluidics has many advantages including variety of sorting principles, precise cell manipulation capability, and combination with downstream analysis. We highlight microfluidic cell sorting and separation techniques and their principles, and establish terminologies and metrics used in their analysis. Lastly, we provide perspective of potential future applications or directions for microtechnologies.

Keywords

  • Cell sorting
  • Cell separation
  • Microfluidics
  • Magnetophoresis
  • Dielectrophoresis
  • Optical sorting
  • Acoustic sorting
  • Gravity-driven cell manipulation
  • Inertial microfluidics
  • Aqueous two-phase system
  • FACS
  • MACS
  • Centrifugation
  • Filtration
  • Terminology

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  • DOI: 10.1007/978-3-319-44139-9_1
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Authors and Affiliations

  1. Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-Gu, Daejeon, 34141, Korea

    Wonhee Lee

  2. Tufts University, 200 Boston Ave, Medford, MA, 02155, USA

    Peter Tseng

  3. Department of Bioengineering, California NanoSystems Institute, Jonsson Comprehensive Cancer Center, University of California, Los Angeles, 420 Westwood Plaza, Los Angeles, CA, 90095, USA

    Dino Di Carlo

Authors
  1. Wonhee Lee
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  2. Peter Tseng
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  3. Dino Di Carlo
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Corresponding authors

Correspondence to Wonhee Lee , Peter Tseng or Dino Di Carlo .

Editor information

Editors and Affiliations

  1. KAIST, Yuseong-gu, Korea (Republic of)

    Wonhee Lee

  2. Tufts University, Medford, Massachusetts, USA

    Peter Tseng

  3. University of California, Los Angeles, Los Angeles, California, USA

    Dino Di Carlo

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Lee, W., Tseng, P., Di Carlo, D. (2017). Microfluidic Cell Sorting and Separation Technology. In: Lee, W., Tseng, P., Di Carlo, D. (eds) Microtechnology for Cell Manipulation and Sorting. Microsystems and Nanosystems. Springer, Cham. https://doi.org/10.1007/978-3-319-44139-9_1

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  • DOI: https://doi.org/10.1007/978-3-319-44139-9_1

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-44137-5

  • Online ISBN: 978-3-319-44139-9

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