Abstract
In this laboratory exercise, sarcoplasmic muscle proteins of fish are extracted with a salt solution, the protein content of the extract is measured by the bicinchoninic acid (BCA) colorimetric assay, and the proteins in the fish extracts are separated and visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Visualization of the protein banding patterns (subunit size and relative quantity) makes it possible to distinguish among different types of fish since many fish have a characteristic protein subunit pattern.
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Bio-Rad (2013) A Guide to Polyacrylamide Gel Electrophoresis and Detection. Bulletin 6040 Rev B. Bio-Rad Laboratories, http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_6040.pdf Accessed June 24, 2015
Chang SKC, Zhang Y (2017) Protein analysis. Ch. 18. In: Nielsen SS (ed) Food analysis, 5th edn. Springer, New York
Etienne M et al. (2000) Identification of fish species after cooking by SDS-PAGE and urea IEF: a collaborative study. J Agr Food Chem 48:2653–2658
Etienne M et al. (2001) Species identification of formed fishery products and high pressure-treated fish by electrophoresis: a collaborative study. Food Chem 72:105–112
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Olsen BJ and Markwell J (2007) Assays for the determination of protein concentration. Unit 3.4 Basic Protocol 3. BCA Assay. Current Protocols in Protein Science John Wiley and Sons, New York
Pierce (2013) Instructions: Pierce BCA protein assay kit. Pierce Biotechnology, Rockford, IL https://tools.lifetechnologies.com/content/sfs/manuals/MAN0011430_Pierce_BCA_Protein_Asy_UG.pdf Accessed June 24, 2015
Piñeiro C et al (1999) Development of a sodium dodecyl sulfate-polyacrylamide gel electrophoresis reference method for the analysis and identification of fish species in raw and heat-processed samples: a collaborative study. Electrophoresis 20:1425–1432
Smith DM (2017) Protein separation and characterization. Ch. 24. In: Nielsen SS (ed) Food analysis, 5th edn. Springer, New York
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Smith, D.M. (2017). Proteins: Extraction, Quantitation, and Electrophoresis. In: Food Analysis Laboratory Manual. Food Science Text Series. Springer, Cham. https://doi.org/10.1007/978-3-319-44127-6_23
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DOI: https://doi.org/10.1007/978-3-319-44127-6_23
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