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Systems Biology and Stem Cell Pluripotency: Revisiting the Discovery of Induced Pluripotent Stem Cell

  • Kaveh Mashayekhi
  • Vanessa Hall
  • Kristine Freude
  • Miya K Hoeffding
  • Luminita Labusca
  • Poul HyttelEmail author
Chapter

Abstract

Recent breakthroughs in stem cell biology have accelerated research in the area of regenerative medicine. Over the past years, it has become possible to derive patient-specific stem cells which can be used to generate different cell populations for potential cell therapy. Systems biological modeling of stem cell pluripotency and differentiation have largely been based on prior knowledge of signaling pathways, gene regulatory networks, and epigenetic factors. However, there is a great need to extend the complexity of the modeling and to integrate different types of data, which would further improve systems biology and its uses in the field. In this chapter, we first give a general background on stem cell biology and regenerative medicine. Stem cell potency is introduced together with the hierarchy of stem cells ranging from pluripotent embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) to tissue-specific multipotent and unipotent stem cells. Secondly, we address some of the systems biological approaches which have already added valuable knowledge to the stem cell field. Particular attention is paid to the most commonly used knowledge-based models as well as to the unsupervised data-driven model. Finally, we will revisit the discovery of the iPSCs by Yamanaka in 2006 and superimpose a data-driven systems biological approach on the data which this amazing discovery was based on. This approach helps to demonstrate how systems biology can complement the field of stem cell biology.

Keywords

Systems biology Stem cells Pluripotency Cell differentiation Regenerative medicine 

Notes

Acknowledgments

We thank the following for financial support: The Danish National Advanced Technology Foundation (project number 047-2011-1; patient-specific stem cell-derived models for Alzheimer’s disease) and the European Union 7th Framework Program (PIAP-GA-2012-324451-STEMMAD) and Innovation Fund Denmark, BrainStem.

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Kaveh Mashayekhi
    • 1
  • Vanessa Hall
    • 2
  • Kristine Freude
    • 2
  • Miya K Hoeffding
    • 3
  • Luminita Labusca
    • 4
    • 5
  • Poul Hyttel
    • 2
    Email author
  1. 1.iGenomix S.L.Parc Cientific Universitat de ValenciaPaternaSpain
  2. 2.Department of Veterinary Clinical and Animal SciencesUniversity of CopenhagenFrederiksberg CDenmark
  3. 3.Copenhagen Consortium for Designer OrganismsUniversity of CopenhagenKoebenhavn NDenmark
  4. 4.Orthopedic and Traumatology ClinicEmergency University Hospital, Saint Spiridon Piaţa Independenţei 1IaşiRomania
  5. 5.National Institute of Research and Development for Technical PhysicsIasiRomania

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