Abstract
Microsatellites, also referred to as short tandem repeats (STRs) or simple sequence repeats (SSRs) are short sequences of tandem repeats of 1–6 bp in length in clusters of less than 150 bp flanked by sections of non-repetitive unique sequences that are scattered throughout the nuclear genome. These markers are co-dominant and hypervariable, revealing many alleles per locus; they are inherited in Mendelian fashion making them useful for detecting genetic variability within species. Once isolated and characterized, microsatellites can also be used in closely related taxa. Microsatellites can be amplified, even from highly degraded DNA, and are very simple to score. More importantly, these markers are highly polymorphic due to the plethora of variations in the repeat motifs. Several studies have endorsed microsatellite markers as an effective genetic tool to determine the historical distinctiveness of populations, and hence, the designation of species. Being highly polymorphic and selectively neutral, microsatellite markers offer a powerful genetic tool for investigating population structure, colonization processes, temporal and spatial population dynamics and evolutionary trends of insect pests. Furthermore, these markers have been successfully applied to different invasive fruit fly species to infer the evolutionary aspects underlying their invasive processes. Microsatellite markers have offered an analytical tool for the study of fruit fly invasion genetics as exemplified for the Mediterranean fruit fly, Ceratitis capitata. Herein, a detailed utility of microsatellite markers in inferring invasion histories of key fruit flies of economic importance is given, with a special focus on invasion into Africa of Bactrocera dorsalis.
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Abdelkrim J, Robertson BC, Stanton J-A L, Gemmell NJ (2009) Fast, cost-effective development of species-specific microsatellite markers by genomic sequencing. BioTechniques 46:185–192
Abdul-Muneer PM (2014) Application of Microsatellite Markers in Conservation Genetics and Fisheries Management: Recent Advances in Population Structure Analysis and Conservation strategies. Hindawi Publishing Corporation, Gen Res Intern Volume 2014, Article ID 691759, 11. http://dx.doi.org/10.1155/2014/691759
Adsavakulchai A, Baimai V, Prachyabrued W, Grote PJ, Lertlum S (1999) Morphometric study for identification of the Bactrocera dorsalis complex (Diptera: Tephritidae) using wing image analysis. Biotropica 13:37–48
Aketarawong N, Bonizzoni M, Malacrida AR, Gasperi G, Thanaphum S (2006) Seventeen novel microsatellite markers from an enriched library of the pest species Bactrocera dorsalis sensu stricto. Mol Ecol Notes 6:1138–1140
Aketarawong N, Bonizzoni M, Thanaphum S, Gomulski LM, Gasperi G, Malacrida AR, Gugliemino CR (2007) Inferences on the population structure and colonization process of the invasive oriental fruit fly, Bactrocera dorsalis (Hendel). Mol Ecol 16:3522–3532
Aketarawong N, Guglielmino CR, Karam N, Falchetto M, Manni M, Scolari F, Gomulski LM, Gasperi G, Malacrida AR (2014) The oriental fruit fly Bactrocera dorsalis s.s. in East Asia: disentangling the different forces promoting the invasion and shaping the genetic make-up of populations. Genetica 142:201–213
Aluja M, Norrbom AL (2000) Fruit flies (Tephritidae): phylogeny and evolution of behavior. CRC Press, Boca Raton
Armstrong KF, Cameron CM, Frampton ER (1997) Fruit fly (Diptera: Tephritidae) species identification: a rapid molecular diagnostic technique for quarantine application. Bull Entomol Res 87:111–118
Baliraine FN, Bonizzoni M, Osir EO, Lux SA, Mulaa FJ, Zheng L, Gomulski LM, Gasperi G, Malacrida AR (2002) Comparative analysis of microsatellite loci in four fruit fly species of the genus Ceratitis (Diptera: Tephritidae). In: Proceedings of 6th international fruit fly symposium, 6–10 May 2002, Stellenbosch, South Africa
Baliraine FN, Bonizzoni M, Osir EO, Lux SA, Mulaa FJ, Zheng L, Gomulski LM, Gasperi G, Malacrida AR (2003) Comparative analysis of microsatellite loci in four fruit fly species of the genus Ceratitis (Diptera: Tephritidae). Bull Entomol Res 93:1–10
Baliraine FN, Bonizzoni M, Guglielmino CR, Osir EO, Lux SA, Mulaa FJ, Gomulski LM, Quilici S, Gasperi G, Malacrida AR (2004) Population genetics of the potentially invasive African fruit fly species, Ceratitis rosa and Ceratitis fasciventris (Diptera: Tephritidae). Mol Ecol 13:683–695
Barr NB, Copeland RS, De Meyer M, Masiga D, Kibogo HG, Billah MK, Osir E, Wharton RA, McPheron BA (2006) Molecular diagnostics of economically important Ceratitis fruit fly species (Diptera: Tephritidae) in Africa using PCR and RFLP analyses. Bull Entomol Res 96:505–521
Baruffi L, Damiani G, Guglielmino CR, Malacrida AR, Bandi C, Gasperi G (1995) Polymorphism within and between populations of Ceratitis capitata: comparison between RAPD and multilocus enzyme electrophoresis. Heredity 74:425–437
Bonizzoni M, Malacrida AR, Guglielmino CR, Gomulski LM, Gasperi G, Zheng L (2000) Microsatellite polymorphism in the Mediterranean fruit fly Ceratitis capitata. Insect Mol Biol 9:251–261
Bonizzoni M, Zheng L, Guglielmino CR, Haymer DS, Gasperi G, Gomulski LM, Malacrida AR (2001) Microsatellite analysis of medfly bioinfestations in California. Mol Ecol 10:2515–2524
Bonizzoni M, Guglielmino CR, Smallridge CJ, Gomulski LM, Malacrida AR, Gasperi G (2004) On the origins of medfly invasion and expansion in Australia. Mol Ecol 13:3845–3855
Boykin LM, Schutze MK, Krosch MN, Chomic A, Chapman TA, Englezou A, Armstrong KF, Clarke AR, Hailstones D, Cameron SL (2014) Multi-gene phylogenetic analysis of south-east Asian pest members of the Bactrocera dorsalis species complex (Diptera: Tephritidae) does not support current taxonomy. J Appl Entomol 138:235–253
Bruford MW, Wayne RK (1993) Microsatellite and their application to population genetic studies. Curr Opin Genet De 3:939–943
Castelo AT, Martins W, Gao GR (2002) TROLL-tandem repeat occurrence locator. Bioinformatics 18:634–636
Chen H, Pulido JC, Duyk GM (1995) MATS: A rapid and efficient method for the development of microsatellite markers from YACs. Genomics 25:1–8
Clarke AR, Armstrong KF, Carmichael AE, Milne JR, Raghu S, Roderick GK, Yeates DK (2005) Invasive phytophagous pests arising through a recent evolutionary radiation: the Bactrocera dorsalis complex of fruit flies. Ann Rev Entomol 50:293–319
Copeland RS, White IM, Okumu M, Machera P, Wharton RA (2004) Insects associated with fruits of the Oleaceae (Asteridae, Lamiales) in Kenya, with special reference to the Tephritidae (Diptera). Bishop Mus Bull Entomol 12:135–164
De Meyer M (1998) Revision of the subgenus Ceratitis (Ceratalaspis) Hancock, 1918 (Diptera: Tephritidae, Ceratitini). Bull Entomol Res 88:257–290
De Meyer M (2005) Phylogenetic relationships within the fruit fly genus Ceratitis MacLeay (Diptera: Tephritidae), derived from morphological and host plant evidence. Insect Syst Evol 36:459–480
De Meyer M, Copeland RS, Wharton RA, McPheron BA (2002) On the geographical origin of the medfly, Ceratitis capitata (Wiedemann). Abstract presented at the 6th international symposium on fruit flies of economic importance, Stellenbosch, South Africa, 6–10 May 2002.
De Meyer M, Robertson MP, Mansell MW, Ekesi S, Tsuruta K, Mwaiko W, Vayssières JF, Peterson AT (2009) Ecological niche and potential geographic distribution of the invasive fruit fly Bactrocera invadens (Diptera, Tephritidae). Bull Entomol Res 100:35–48
Drew RAI, Tsuruta K, White IM (2005) A new species of pest fruit fly (Diptera: Tephritidae: Dacinae) from Sri Lanka and Africa. Afri Entomol 13:149–154
Drew RAI, Dorji C, Romig MC, Loday P (2006) Attractiveness of various combinations of colors and shapes to females and males of Bactrocera minax (Diptera: Tephritidae) in a commercial mandarin grove in Bhutan. J Econ Entomol 99:1651–1656
Drew RAI, Raghu S, Halcoop P (2008) Bridging the morphological and biological species concepts: studies on the Bactrocera dorsalis (Hendel) complex (Diptera: Tephritidae: Dacinae) in South-east Asia. Biol J Linn Soc 93:217–226
Edwards A, Civitello A, Hammond HA, Caskey CT (1991) DNA typing and genetic mapping with trimeric and tetrameric tandem repeats. Am J Hum Genet 49:746–756
Edwards KJ, Barker JHA, Daly A, Jones C, Karp A (1996) Microsatellite libraries enriched for several microsatellite sequences in plants. Biotechniques 20:758–760
Ekesi S, Billah MK (2007) A field guide to the management of economically important Tephritid fruit flies in Africa. 2nd ed, icipe Science press B1–I4
Ekesi S, Nderitu P, Rwomushana I (2006) Field infestation, life history and demographic parameters of the fruit fly Bactrocera invadens (Diptera: Tephritidae) in Africa. Bull Entomol Res 96:379–386
Estoup A, Jarne P, Cornuet JM (2002) Homoplasy and mutation model at microsatellite loci and their consequences for population genetics analysis. Mol Ecol 11:1591–1604
European and Mediterranean Plant Protection Organization, EPPO (2009) Bactrocera invadens (Diptera: Tephritidae) http://www.eppo.org/QUARANTINE/Alert_List/insects/BCTRIN.htm
Faircloth BC (2008) MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Resour 8:92–94
Fletcher BS (1989) Movements of tephritid fruit flies. In: Robinson AS, Hooper GH (eds) Fruit flies: their biology, natural enemies and control, vol 3B. Elsevier Science Publications, Amsterdam, pp 209–219
French C (2005) The new invasive Bactrocera species. pp. 19–20 In: Insect pest control newsletter, No. 65. International Atomic Energy Agency, Vienna, Austria
Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Method Enzymol 395:202–222
Golein B, Talaie A, Zamani Z, Moradi B (2006) Development and characterization of new microsatellite loci from lemon (Citrus limon). Int J Agr Biol 8:172–174
Gomulski LM, Bourtzis K, Brogna S, Morandi PA, Bonvicini C, Sebastiani F, Torti C, Guglielmino CR, Savakis C, Gasperi G, Malacrida AR (1998) Intron size polymorphism of the Adh1 gene parallels the worldwide colonization history of the Mediterranean fruit fly, Ceratitis capitata. Mol Ecol 7:1729–1742
Gupta PK, Rustgi S, Sharma S, Singh R, Kumar N, Balyan HS (2003) Transferable EST-SSR markers for the study of polymorphism and genetic diversity in bread wheat. Mol Genet Genomics 270:315–323
Hamada HM, Petrino MG, Kakunaga T (1982) A novel repeated element with Z-DNA forming potential is widely found in evolutionarily diverse eukaryotic genomes. PNAS 79:6465–6469
Han HY, McPheron BA (1997) Molecular phylogenetic study of Tephritidae (Insecta: Diptera) using partial sequences of the mitochondrial 16S ribosomal DNA. Mol Phyl Evol 7:17–32
He GH, Meng RH, Newman M, Gao G, Pittman RN, Prakash CS (2003) Microsatellites as DNA markers in cultivated peanut (Arachis hypogaea L.). BMC Plant Biol 3:3
Hedrick PW, Parker KM, Lee RN (2001) Using microsatellite and MHC variation to identify species, ESUs, and Mus in endangered Sonoran topminnow. Mol Ecol 10:1399–1412
Innan H, Terauchi R, Miyashita NT (1997) Microsatellite polymorphism in natural populations of the wild plant Arabidopsis thaliana. Genetics 146:1441–1452
Jacob HJ, Lindpaintner K, Lincoln SE, Kusumi K, Bunker RK, Mao YP, Ganten D, Dzau VJ, Lander ES (1991) Genetic mapping of a gene causing hypertensive rat. Cell 67:213–224
Jarne P, Lagoda PJL (1996) Microsatellites, from molecules to populations and back. TREE 11:424–429
Kandpal RP, Kandpal G, Weissman SM (1994) Construction of libraries enriched for sequence repeats and jumping clones, and hybridization selection for region-specific markers. PNAS 91:88–92
Karagyozov L, Kalcheva ID, Chapman VM (1993) Construction of random small-insert genomic libraries highly enriched for simple sequence repeats. Nucleic Acids Res 21:3911–3912
Khamis F, Karam N, Guglielmino CR, Ekesi S, Masiga D, De Meyer M, Kenya EU, Malacrida AR (2008) Isolation and characterization of microsatellite markers in the newly discovered invasive fruit fly pest in Africa, Bactrocera invadens (Diptera: Tephritidae). Mol Ecol Res 8:1509–1511
Khamis FM, Karam N, Ekesi S, De Meyer M, Bonomi A, Gomulski LM, Scolari F, Gabrieli P, Siciliano P, Masiga D, Kenya EU, Gasperi G, Malacrida AR, Guglielmino CR (2009) Uncovering the tracks of a recent and rapid invasion: the case of the fruit fly pest Bactrocera invadens (Diptera: Tephritidae) in Africa. Mol Ecol 18:4798–4810
Khamis FM, Masiga DK, Mohamed SA, Salifu D, De Meyer M, Ekesi S (2012) Taxonomic identity of the invasive fruit fly pest, Bactrocera invadens: concordance in morphometry and DNA barcoding. PLoS One 7:e44862
Kinyanjui G, Khamis FM, Mohamed S, Ombura LO, Warigia M, Ekesi S (2016) Identification of aphid (Hemiptera: Aphididae) species of economic importance in Kenya using DNA barcodes. Bull Entomol Res 106:63–72. doi:10.1017/S0007485315000796
Kofler R, Schlotterer C, Lelley T (2007) SciRoKo: a new tool for whole genome microsatellite search and investigation. Biogeosciences 23:1683–1685
Kumar P, Gupta VK, Misra AK, Modi DR, Pandey BK (2009) Potential of molecular markers in plant biotechnology. Plant Omics J 2:141–162
Lee JM, Nahm SH, Kim YM, Kim BD (2004) Characterization and molecular genetic mapping of microsatellite loci in pepper. Theor Appl Genet 108:619–627
Levinson G, Gutman GA (1987) Slipped-strand mispairing: a major mechanism for DNA sequence evolution. Mol Biol Evol 4:203–221
Litt M, Luty JA (1989) A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. Am J of Hum Gen 44:397–401
Loxdale HD, Lushai G (1998) Molecular markers in entomology. Bull Entomol Res 88:557–600
Lux SA, Copeland RS, White IM, Manrakhan A, Billah MK (2003) A new invasive fruit fly species from the Bactrocera dorsalis (Hendel) group detected in East Africa. Insect Sci Appl 23:355–361
Malacrida AR, Guglielmino CR, D’Adamo P, Torti C, Marinoni F, Gasperi G (1996) Allozyme divergence and phylogenetic relationships among species of tephritid flies. Heredity 76:592–602
Malacrida AR, Marinoni F, Torti C, Gomulski LM, Sebastiani F, Bonvicini C, Gasperi G, Guglielmino CR (1998) Genetic aspects of the worldwide colonization process of Ceratitis capitata. J Hered 89:501–507
Malacrida AR, Gomulski LM, Bonizzoni M, Bertin S, Gasperi G, Guglielmino CR (2007) Globalization and fruit fly invasion and expansion: the medfly paradigm. Genetica. doi:10.1007/s10709-006-9117-2
Manni M, Lima KM, Guglielmino CR, Lanzavecchia SB, Juri M, Vera T, Cladera J, Scolari F, Gomulski L, Bonizzoni M, Gasperi G, Silva JG, Malacrida AR (2015) Relevant genetic differentiation among Brazilian populations of Anastrepha fraterculus (Diptera, Tephritidae). ZooKeys 540:157–173
McPheron BA (2000) Population genetics and cryptic species. In: Tan KH (ed) Area-wide control of fruit flies and other insect pests. Penerbit Universiti Sains Malaysia, Penang, pp 483–490
Meixner MD, McPheron BA, Silva JG, Gasparich GE, Sheppard WS (2002) The Mediterranean fruit fly in California: evidence for multiple introductions and persistent populations based on microsatellite and mitochondrial DNA variability. Mol Ecol 11:891–899
Morrow J, Scott L, Congdon B, Yeates D, Frommer M, Sved J (2000) Close genetic similarity between two sympatric species of tephritid fruit fly reproductively isolated by mating time. Evolution 54:899–910
Mwatawala MW, White IM, Maerere AP, Senkondo FJ, De Meyer M (2004) A new invasive Bactrocera species (Diptera: Tephritidae) in Tanzania. Afri Entomol 12:154–156
Mwatawala MW, De Meyer M, Makundi RH, Maerere AP (2006) Biodiversity of fruit flies (Diptera, Tephritidae) at orchards in different agro-ecological zones of the Morogoro region, Tanzania. Fruits 61:321–332
Nardi F, Carapelli A, Dallai R, Roderick GK, Frati F (2005) Population structure and colonization history of the olive fly, Bactrocera oleae (Diptera, Tephritidae). Mol Ecol 14:2729–2738
Ostrander EA, Jong PM, Rine J, Duyk G (1992) Construction of small-insert genomic DNA libraries highly enriched for microsatellite repeat sequences. PNAS 89:3419–3423
Paetkau D (1999) Microsatellites obtained using strand extension: an enrichment protocol. Biotechniques 26:690–697
Pandolfo M (1992) A rapid method to isolate (GT)n repeats from yeast artificial chromosomes. Nucleic Acids Res 20:1154
Piry S, Alapetite A, Cornuet JM, Paetkau D, Baudouin L, Estoup A (2004) Geneclass 2: a software for genetic assignment and first s – generation migrant detection. J Hered 95:536–539
Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotypic data. Genetics 155:945–959
Rassmann K, Schlotterer C, Tautz D (1991) Isolation of simple sequence loci for use in polymerase chain reaction-based DNA fingerprinting. Electrophoresis 12:113–118
Roderick G, Navajas M (2003) Genotypes in novel environments: genetics and evolution in biological control. Nat Rev Gen 4:889–899
Rudd S (2003) Expressed sequence tags: alternative or complement to whole genome sequences? Trends Plant Sci 8:321–329
Schlötterer C, Tautz D (1992) Slippage synthesis of simple sequence DNA. Nuc Acids Res 20:211–215
Schuler GD, Boguski MS, Stewart EA, Stein LD, Gyapay G, Rice K, White RE, Rodriguez-Tomé P, Aggarwal A, Bajorek E, Bentolila S, Birren BB, Butler A, Castle AB, Chiannilkulchai N, Chu A, Clee C, Cowles S, Day PJ, Dibling T, Drouot N, Dunham I, Duprat S, East C, Edwards C, Fan JB, Fang N, Fizames C, Garrett C, Green L, Hadley D, Harris M, Harrison P, Brady S, Hicks A, Holloway E, Hui L, Hussain S, Louis-Dit-Sully C, Ma J, MacGilvery A, Mader C, Maratukulam A, Matise TC, McKusick KB, Morissette J, Mungall A, Muselet D, Nusbaum HC, Page DC, Peck A, Perkins S, Piercy M, Qin F, Quackenbush J, Ranby S, Reif T, Rozen S, Sanders C, She X, Silva J, Slonim DK, Soderlund C, Sun WL, Tabar P, Thangarajah T, Vega-Czarny N, Vollrath D, Voyticky S, Wilmer T, Wu X, Adams MD, Auffray C, Walter NA, Brandon R, Dehejia A, Goodfellow PN, Houlgatte R, Hudson JR Jr, Ide SE, Iorio KR, Lee WY, Seki N, Nagase T, Ishikawa K, Nomura N, Phillips C, Polymeropoulos MH, Sandusky M, Schmitt K, Berry R, Swanson K, Torres R, Venter JC, Sikela JM, Beckmann JS, Weissenbach J, Myers RM, Cox DR, James MR, Bentley D, Deloukas P, Lander ES, Hudson TJ (1996) A gene map of the human genome. Science 274:540–546
Schutze MK, Aketarawong N, Amornsak W, Armstrong KF, Augustinos AA, Barr N, Bo W, Bourtzis K, Boykin LM, Cáceres C, Cameron SL, Chapman TA, Chinvinijku S, Chomiˇc A, De Meyer M, Drosopoulou E, Englezou A, Ekesi S, Gariou- Papalexiou A, Geib SM, Hailstones D, Hasanuzzaman M, Haymer D, Hee AKW, Hendrichs J, Jessup A, Ji Q, Khamis FM, Krosch MN, Leblanc L, Mahmood K, Malacrida AR, Mavragani-Tsipidou P, Mwatawala M, Nishida R, Ono H, Reyes J, Rubinoff D, San Jose M, Shelly TE, Srikachar S, Tan KH, Thanaphum S, Haq I, Vijaysegaran S, Wee SL, Yesmin F, Zacharopoulou A, Clarke AR (2014) Snonymization of key pest species within the Bactrocera dorsalis species complex (Diptera: Tephritidae): Taxonomic changes based on a review of 20 years of integrative morphological, molecular, cytogenetic, behavioural and chemoecological data. System Entomol. doi:10.1111/syen.12113
Senan S, Kizhakayil D, Sasikumar B, Sheeja TE (2014) Methods for development of microsatellite markers: an overview. Not Sci Biol 6:1–13
Shearman DCA, Gilchrist AS, Crisafulli D, Graham G, Lange C, Frommer M (2006) Microsatellite markers for the pest fruit fly, Bactrocera papayae (Diptera: Tephritidae) and other Bactrocera species. Mol Ecol Notes 6:4–7
Sonvinco A, Manso F, Quesada-Allue LA (1996) Discrimination between the immature stages of Ceratitis capitata and Anastrepha fraterculus (Diptera: Tephritidae) populations by random amplified polymorphic DNA polymerase chain reaction. J Econ Entomol 89:1208–1212
Thiel T, Michalek W, Varshney RK, Graner A (2003) Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.). Theor Appl Genet 106:411–422
Wang R, Zheng L, Touré YT, Dandekar T, Kafatos F (2001) When genetic distance matters: measuring genetic differentiation at microsatellite loci in whole-genome scans of recent and incipient mosquito species. PNAS 98:10769–10774
Weber JL, May PE (1989) Abundant class of human DNA polymorphism which can be typed using polymerase chain reaction. Am J Hum Gen 44:388–396
White IM, Elson-Harris MM (1992) Fruit flies of economic significance: their identification and bionomics. C.A.B International and Australian Centre for International Agricultural Research, Canberra, p 601
White G, Powell W (1997) Isolation and characterization of microsatellite loci in Swietenia humilis (Meliaceae): an endangered tropical hardwood species. Mol Ecol 6:851–860
Wu Y, Li Z, Wu J (2009) Polymorphic microsatellite markers in the melon fruit fly, Bactrocera cucurbitae (Coquillett) (Diptera: Tephritidae). Mol Ecol Resour 9:1404–1406
Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16
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Research activities were funded by grants from GIZ/BMZ, Biovision, the EU, IAEA, DFID and DAAD to the African Fruit Fly Programme (AFFP) of icipe.
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Khamis, F.M., Malacrida, A.R. (2016). Role of Microsatellite Markers in Molecular Population Genetics of Fruit Flies with Emphasis on the Bactrocera dorsalis Invasion of Africa. In: Ekesi, S., Mohamed, S., De Meyer, M. (eds) Fruit Fly Research and Development in Africa - Towards a Sustainable Management Strategy to Improve Horticulture. Springer, Cham. https://doi.org/10.1007/978-3-319-43226-7_4
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