Daily, Weekly, and Regular Preparations for the IVF Laboratory
This chapter will discuss the various rooms or areas of specialization found within an IVF embryology laboratory today and how each room or area should be monitored on a daily, weekly, and regular basis. Standards for each room and piece of equipment should be established so equipment is functioning at its optimum in order to minimize any impact possible on the developing embryos. Topics such as suggestions for frequency in monitoring temperatures, gas levels, and pH measurements will be discussed in detail. Differences in terms of incubator monitoring between benchtop and big box incubators will be discussed as well. Parameters used for gas manifold room measurements will also be reviewed in addition to the HVAC room. Frequency of filter inspections and changes will be discussed. Ultimately, laboratories will have to establish standards by determining the ideal ranges of acceptable variability through empirical tests designed to optimize the laboratory environment for growing healthy embryos within that specific environment.
KeywordsPreparations IVF Laboratory Monitoring temperatures Gas levels pH measurements
- 1.The Practice Committee of the American Society of Reproductive Medicine and the Practice Committee of the Society for Assisted reproductive Technology. Revised guidelines for human embryology and andrology laboratories. Fertil Steril. 2008;90:S45–59.Google Scholar
- 3.Wiemer KE, Anderson A, Weikert L. Quality control in the IVF laboratory. In: Dunitz M, editor. Textbook of assisted reproductive techniques. London: Martin Dunitz Ltd; 2001. p. 27–34.Google Scholar