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Processing of Sperm Samples in HIV-Positive Patients

  • Rocio Rivera-Egea
  • Thamara Viloria Samochin
  • Marcos Meseguer EscriváEmail author
Chapter

Abstract

Human immunodeficiency virus (HIV) is an important public health problem. Approximately 37 million people around the world are infected with HIV type 1 virus. In recent years, prolonged survival of patients receiving highly active antiretroviral therapy has given rise to a number of couples in which only one member is HIV positive (serodiscordant (SDC) couples). This increase has been accompanied by growth of a new demand from SDC couples who wish to have children; therefore, such couples in which the male is infected need to make use of assisted reproduction technology (ART), in combination with semen washing, to decrease the risk of HIV transmission to the partner or to the newborn. Thus, it is essential to test the effectiveness of techniques aimed at reducing HIV and hepatitis C virus (HCV) loads in infected semen, using semen washing and molecular biology tests to eliminate the presence of the virus. This option has been demonstrated to be effective and is widely used in such cases. Semen washing consists of strict selection of the best motile sperm, removing tiny amounts of other cell types and seminal plasma, given that sperm seminal plasma and nonspermatic cells are considered the main reservoir of HIV. Our aim in this chapter is to compile the available information about sperm washing and ART with sperm samples obtained from SDC males with HIV, to describe the current possibilities for these men.

Keywords

Sperm HIV Assisted reproduction PCR Sperm sample processing HIV and assisted reproduction 

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Rocio Rivera-Egea
    • 1
  • Thamara Viloria Samochin
    • 2
  • Marcos Meseguer Escrivá
    • 3
    Email author
  1. 1.Andrology Laboratory and Sperm Bank, IVIRMA ValenciaValenciaSpain
  2. 2.IVF Laboratory, Instituto Universitario IVI ValenciaValenciaSpain
  3. 3.IVF LaboratoryIVI ValenciaValenciaSpain

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