Abstract
Long single-stranded (ss) DNAs generated by rolling circle amplification (RCA) are attractive vehicles for cellular delivery of drug and/or cellular probes since they can be readily loaded by multiple chemical entities via variety of means. However, they cannot be used for this purpose just such as they are because large and loose DNA particles they normally form could not easily enter into cells. Through DNA origami technology, RCA-generated single-stranded (ss) DNA can be used as scaffolds to produce the condensed two-dimensional (2D) DNA nanoribbons. Besides, the RCA-based DNA origami can be assembled on the surface of gold nanoparticles to create the three-dimensional (3D) DNA-gold nanoribbons. Compared to the conventional DNA origami, RCA offers the design flexibility of both scaffold and staple sequences. Particularly, the assembly of DNA nanoribbons is greatly simplified due to the repetitive sequences in RCA amplicons allowing the involvement of only few staple strands. We demonstrate here that both the 2D DNA nanoribbon and the 3D DNA-gold nanoribbon obtained with the use of RCA can serve as high-efficiency nanocarriers for cellular probe and drug delivery.
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© 2016 Springer International Publishing Switzerland
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Song, S., Fan, C. (2016). RCA-Assisted Self-assembled DNA Origami Nano-constructs as Vehicles for Cellular Delivery of Diagnostic Probes and Therapeutic Drugs. In: Demidov, V. (eds) Rolling Circle Amplification (RCA). Springer, Cham. https://doi.org/10.1007/978-3-319-42226-8_13
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DOI: https://doi.org/10.1007/978-3-319-42226-8_13
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