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DNA Nanoclews for Stimuli-Responsive Anticancer Drug Delivery

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Rolling Circle Amplification (RCA)
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Abstract

The technique of rolling circle amplification (RCA) emerged recently as a robust tool to generate the regularly patterned DNA structures in addition to its long-recognized ability of amplifying biological signals. Here, we demonstrate the strategy of RCA-driven generation of nano-scaled cocoon-like DNA particles, designated as DNA nanoclews, for loading and delivering a small molecule anticancer drug. The characteristic biodegradability of DNA was harnessed to control the timing and location of drug release by utilizing a polymeric nanogel encapsulating DNA-degrading enzyme DNase I. This smart drug delivery system based on RCA amplicons could get internalized into targeted cells and release the cargo using the endosomal acidity as a trigger.

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Acknowledgements

This work was supported by the grant from NC TraCS, NIH’s Clinical and Translational Science Awards (CTSA, 1UL1TR001111) at UNC-CH, the NC State Faculty Research and Professional Development Award, and the start-up package from the Joint BME Department of UNC-CH and NCSU to Z.G.

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Correspondence to Wujin Sun or Zhen Gu Ph.D. .

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Sun, W., Gu, Z. (2016). DNA Nanoclews for Stimuli-Responsive Anticancer Drug Delivery. In: Demidov, V. (eds) Rolling Circle Amplification (RCA). Springer, Cham. https://doi.org/10.1007/978-3-319-42226-8_12

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