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Mitochondrial DNA Diversity of Wild and Hatchery Reared Strains of Indian Lates calcarifer (Bloch)

  • Prasanna Kumar
  • B. Akbar JohnEmail author
  • V. Kanagasabapathy
Chapter

Abstract

Lates calcarifer, locally known as seabass in Asia and barramundi in Australia, is a large, euryhaline member of the family Centropomidae that is widely distributed in the Indo-West Pacific region. Its hardy nature, high tolerance to wide range physiological condition and high commercial value has made it a good candidate species for aquaculture practices. In this study we compared the mtDNA diversity of hatchery reared and wild Lates calcarifer using universal DNA barcode gene (Cytochrome Oxidase C subunit 1 gene) to assess the genetic health of L. calcarifer hatchery practices in India. Sampling stations were randomly chosen to cover both East and West coasts of India. The phylogram constructed with COI sequences (n = 88) of L. calcarifer revealed that geographic distributions of clades are not restricted to any particular sampling stations. Gene flow appeared to have transported haplotypes between the clades from their likely origins across the sampled range. Both Nucleotide (π) and haplotyte (h) diversity of wild L. calcarifer was higher in East coast samples compared to West coast samples. The comparative genetic diversity analysis assessed through COI sequences between hatchery reared and wild catches of L. calcarifer showed that the nucleotide diversity of hatchery strains was 2.7 times lesser than that of wild strains, demanding immediate action plans to restore genetic diversity in L. calcarifer hatchery practices in India.

Keywords

Lates calcarifer COI gene DNA barcoding Haplotype diversity Genetic diversity 

Notes

Acknowledgments

This work was partly supported by UGC fellowship. Our special thanks are due to the Dean of Centre of Advanced Studies in Marine Biology (CASMB), Annamalai University for his constant encouragement and University officials for providing the necessary facilities. Our special thanks to RGCA, Tamil Nadu and Takave farms, Maharastra for their helpfulness in sampling. We acknowledge the technical support extended by Priority Life Sciences, Coimbatore, India and Macrogen Inc., North Korea.

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Prasanna Kumar
    • 1
  • B. Akbar John
    • 2
    Email author
  • V. Kanagasabapathy
    • 3
    • 4
  1. 1.Centre of Advanced Study in Marine BiologyAnnamalai UniversityParangipettaiIndia
  2. 2.INOCEM Research Station (IRS), Kulliyyah of ScienceInternational Islamic University of MalaysiaKuantanMalaysia
  3. 3.Central Institute of Brackish Water Aquaculture (ICAR)ChennaiIndia
  4. 4.Unit of Live Feed Culture, Department of ZoologyUniversity of MadrasChennaiIndia

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