Biological Inoculants for Sustainable Intensification of Agriculture in Sub-Saharan Africa Smallholder Farming Systems

  • C. MassoEmail author
  • R. W. Mukhongo
  • M. Thuita
  • R. Abaidoo
  • J. Ulzen
  • G. Kariuki
  • M. Kalumuna


Land degradation in the smallholder farming systems in sub-Saharan Africa is mainly related to insufficient adoption of sustainable agriculture technologies. This study was aimed at investigating the potential of biological inoculants to improve crop yields and control plant diseases in a profitable manner. Three rhizobia inoculants for soybean or common bean, 2 arbuscular mycorrhizae fungi (AMF) for sweet potato, and 2 Trichoderma products for tomato were applied to determine their effect on yields and tomato late blight disease. The study was conducted in Ghana, Kenya, Tanzania, and Uganda, but the treatments varied among the countries. The Rhizobia inoculants produced significant soybean or common bean yield increases in Ghana, Kenya, and Tanzania at p ≤ 0.05 when compared to the untreated control, and an economic analysis of the Ghanaian data found that Legumefix was profitable with a value–cost ratio of >3. There was significant spatial variability in crop yields (coefficients of variation: 37–64 %), indicating a need for further investigation to correct the limiting factors. The sweet potato response to AMF was variable across sites and seasons, and a significant response (p ≤ 0.05) was shown only under drought conditions in a soil with low organic matter content (1.2 %). The Trichoderma inoculants controlled late blight disease in tomatoes significantly better than Ridomil (p ≤ 0.05), a synthetic fungicide currently used by farmers in Kenya. Biological inoculants can therefore improve the productivity of the sub-Saharan Africa smallholder farming systems, and awareness of them should be created for relevant stakeholders to increase understanding and adoption of technologies for sustainable agricultural intensification.


Biological inoculants Smallholder farmers Land degradation Technology adoption Sustainable intensification 



The authors take this opportunity to reiterate their appreciation of the financial support from the Bill & Melinda Gates Foundation through the COMPRO-II project grant to IITA. The authors are also grateful for technical support from the various project partners involved in the data collection. IITA’s institutional support and the Innovative Agriculture Research Initiative’s (iAGRI) facilitation during the development of this article are highly appreciated as well.


  1. Ahmad F et al (2013) Phosphorus-microbes interaction on growth, yield and phosphorus-use efficiency of irrigated cotton. Arch Agron Soil Sci 59(3):341–351. doi: 10.1080/03650340.2011.646994 CrossRefGoogle Scholar
  2. Alarcón D, Bodouroglou C (2011) Agricultural innovations for food security and environmental sustainability in the context of the recent economic crisis: why a gender perspective. Accessed 30 June 2015
  3. Anderson JM, Ingram JSI (eds) (1993) Tropical soil biology and fertility: a handbook of methods, 2nd edn. CAB International, The Cambrian News, Aberstwyth, UKGoogle Scholar
  4. Bhaduri D et al (2014) Primary and secondary nutrients—a boon to defense system against plant diseases. Int J Bioresour Stress Manag 5(3):461–466. doi: 10.5958/0976-4038.2014.00597.1 CrossRefGoogle Scholar
  5. Bhattacharyya P, Tandon HLS (2012) Biofertilizer handbook—research, production, application. Fertilizer Development and Consultation Organization, New Delhi, IndiaGoogle Scholar
  6. Bordeleau LM, Prévost D (1994) Nodulation and nitrogn fixation in extreme conditions. Plant Soil 161:115–125CrossRefGoogle Scholar
  7. Buondonno A et al (1995) Comparing tests for soil fertility. II. The hydrogen peroxide/sulfuric acid treatment as an alternative to the copper/selenium catalyzed digestion process for routine determination of soil nitrogen-kjeldahl. Commun Soil Sci Plant Anal 26(9–10):1607–1619. doi: 10.1080/00103629509369394 CrossRefGoogle Scholar
  8. Chianu J et al (2011) Biological nitrogen fixation and socio-economic factors for legume production in sub-Saharan Africa: a review. Agron Sustain Dev 31(1):139–154. doi: 10.1051/agro/2010004 CrossRefGoogle Scholar
  9. Dittoh SA et al (2012) Improving the effectiveness, efficiency and sustainability of fertilizer use in sub-Saharan Africa—policy research paper number 3. Accessed 30 June 2015
  10. Fattah OA (2013) Effect of mycorrhiza and phosphorus on micronutrients uptake by soybean plant grown in acid soil. Int J Agron Plant Prod 4:429–437Google Scholar
  11. Ghosh N (2003) Promoting bio-fertilizers in Indian agriculture. Institute of Economic Growth, University Enclave. Accessed 25 Apr 2015
  12. Guo Z, Koo J, Wood S (2009) Fertilizer profitability in East Africa: a spatially explicit policy analysis. Paper presented at the International Association of Agricultural Economics Conference, Beijing, 16–22 Aug 2009Google Scholar
  13. Harikumar VS, Potty VP (2002) Arbuscular mycorrhizal inoculation and phosphorus mobility in phosphorus-fixing sweet potato soils. Malays J Soil Sci 11:45–56Google Scholar
  14. Havlin JL et al (2005) Soil fertility and fertilizers—an introduction to nutrient management, 7th edn. Pearson Prentice Hall, Upper Saddle River, NJGoogle Scholar
  15. Heanes DL (1984) Determination of organic C in soils by an improved chromic acid digestion and spectrophotometric procedure. Commun Soil Sci Plant Anal 15:1191–1213. doi: 10.1080/00103628409367551 CrossRefGoogle Scholar
  16. Hu S, Rufty T (2007) Linking arbuscular mycorrhizal fungi with plant health: mechanisms and challenges. Phytopathol 97(7):142Google Scholar
  17. International Fertilizer Industry Association (2009) Fertilizers, climate change and enhancing agricultural productivity sustainably. Accessed 30 June 2015
  18. Jefwa JM et al (2014) Do commercial biological and chemical products increase crop yields and economic returns under smallholder farmer conditions? In: Vanlauwe B, van Asten P, Blomme G (eds) Challenges and opportunities for agricultural intensification of the humid highland systems of sub-Saharan Africa. Springer International Publishing Switzerland, Cham, pp 81–96. doi: 10.1007/978-3-319-07662-1_7 Google Scholar
  19. Khan AA et al (2009) Phosphorus solubilizing bacteria: occurrence, mechanisms and their role in crop production. J Agric Biol Sci 1(1):48–58Google Scholar
  20. Kisaka-Lwayo M et al (2005) Analysis of risk attitudes of smallholder crop farmers in Umbumbulu district, Kwazulu-Natal, South Africa. In: African Crop Science Society (ed) African crop science conference proceedings, Kampala, 5–9 December 2005, vol 7, pp 909–914Google Scholar
  21. Lambers H et al (2008) Plant nutrient-acquisition strategies change with soil age. Trends Ecol Evol 23:95–103CrossRefPubMedGoogle Scholar
  22. Masso C et al (2015) Worldwide contrast in application of bio-fertilizers for sustainable agriculture: lessons for sub-Saharan Africa. J Biol Agric Healthc 5(2):34–50Google Scholar
  23. Mbarga JB et al (2012) Mycoparasitic trichoderma viridae as a bio-control agent against fusarium oxysporum f. sp. adzuki and pythium arrhenomanes and as a growth promoter of soybean. J Crop Prot 29:1452–1459. doi: 10.1016/j.cropro.2010.08.004 Google Scholar
  24. Minde I et al (2008) Improving access and utilization of fertilizers by smallholder farmers in the Limpopo province of South Africa. Accessed 30 June 2015
  25. Mujeri MK et al (2012) Improving the effectiveness, efficiency and sustainability of fertilizer use in South Asia. Global Development Network (GDN). Accessed 30 June 2015
  26. Murphy J, Riley JP (1962) A modified single solution method for determination of phosphate in natural waters. Anal Chim Acta 27:31–36. doi: 10.1016/S0003-2670(00)88444-5 CrossRefGoogle Scholar
  27. Mwanga ROM et al (2011) ‘NASPOT 11’, a sweet potato bred by a participatory plant-breeding approach in Uganda. HortScience 46(2):317–321Google Scholar
  28. Nezomba H et al (2015) Point of no return? Rehabilitating degraded soils for increased crop productivity on smallholder farms in eastern Zimbabwe. Geoderma 239–240:143–155. doi: 10.1016/j.geoderma.2014.10.006 CrossRefGoogle Scholar
  29. Nezomba H et al (2008) Nitrogen fixation and biomass productivity of indigenous legumes for fertility restoration of abandoned soils in smallholder farming systems. S Afr J Plant Soil 25(3):161–171. doi: 10.1080/02571862.2008.10639912 CrossRefGoogle Scholar
  30. Nkonya E et al (2011) Climate risk management through sustainable land management in sub-Saharan Africa. Accessed 30 June 2015
  31. Okalebo JR (2002) Laboratory methods for soil and plant analysis. A working manual, 2nd edn. Tropical Soil Fertility and Biology Program-CIAT and SACRED Africa, NairobiGoogle Scholar
  32. Öpik M, Moora M (2012) Missing nodes and links in mycorrhizal networks. New Phytol 194:304–306. doi: 10.1111/j.1469-8137.2012.04121.x CrossRefPubMedGoogle Scholar
  33. Parewa HP et al (2010) Evaluation of maize cultivars for phosphorus use efficiency in an Inceptisol. Int J Agric Environ Biotechnol 3(2):195–198Google Scholar
  34. Pender J (2004) Development pathways and land management in Uganda. World Dev 32:767–792. doi: 10.1016/j.worlddev.2003.11.003 CrossRefGoogle Scholar
  35. Porcel R, Ruiz-Lozano JM (2004) Arbuscular mycorrhizal influence on leaf water potential, solute accumulation, and oxidative stress in soybean plants subjected to drought stress. J Exp Bot 55(403):1743–1750. doi: 10.1093/jxb/erh188 CrossRefPubMedGoogle Scholar
  36. Rengel Z, Marschner P (2005) Nutrient availability and management in the rhizosphere: Exploiting genotypic differences. New Phytol 168:305–312. doi: 10.1111/j.1469-8137.2005.01558.x CrossRefPubMedGoogle Scholar
  37. Roesti D et al (2006) Plant growth stage, fertiliser management and bio-inoculation of arbuscular mycorrhizal fungi and plant growth promoting rhizobacteria affect the rhizobacterial community structure in rain-fed wheat fields. Soil Biol Biochem 38(5):1111–1120. doi: 10.1016/j.soilbio.2005.09.010 CrossRefGoogle Scholar
  38. Robert M (2006) Global change and carbon cycle: the position of soils and agriculture. In: Roose EJ et al (eds) Soil erosion and carbon dynamics—advances in soil science. Taylor & Francis, New York, pp 3–12Google Scholar
  39. Sheahan M, Barrett CB (2014) Understanding the agricultural input landscape in sub-Saharan Africa—recent plot, household, and community-level evidence. World Bank Group. Accessed 30 June 2015
  40. Sutton MA et al (2013) Our nutrient world: the challenge to produce more food and energy with less pollution. Centre for Ecology and Hydrology (CEH), EdinburghGoogle Scholar
  41. United Nations Industrial Development Organization (2014) Africa industrialization day—agribusinesses’ contribution to food security. Accessed 30 June 2015
  42. van der Heijden MGA et al (2006) The mycorrhizal contribution to plant productivity, plant nutrition and soil structure in experimental grassland. New Phytol 172:739–752. doi: 10.1111/j.1469-8137.2006.01862.x CrossRefPubMedGoogle Scholar
  43. Waddington S et al (2004) Progress in lifting soil fertility in Southern Africa. In: New directions for a diverse planet—proceedings of the 4th International Crop Science Congress, Brisbane, Australia, 26 September–1 October 2004Google Scholar
  44. Wanzala M (2011) The Abuja declaration on fertilizers for an African green revolution—status of implementation at regional and national levels June 2011. The New Partnership for Africa’s Development (NEPAD). Accessed 30 June 2015
  45. World Meteorological Organization (2005) Climate and land degradation. Accessed 30 June 2015
  46. Wolf B (1999) The fertile triangle—the interrelationship of air, water, and nutrients in maximizing soil productivity. Food Products Press, Binghamton, New YorkGoogle Scholar
  47. Woomer PL et al (2014) N2Africa final report of the first phase 2009–2013. Accessed 30 June 2015
  48. Wu JL et al (2004) Sorghum diversity evaluated by simple sequence repeat (SSR) markers and phenotypic performance. Plant Prod Sci 7:301–308. doi: 10.1626/pps.7.301 CrossRefGoogle Scholar
  49. Yaseen T et al (2011) Effect of arbuscular mycorrhiza inoculation on nutrient uptake, growth and productivity of cowpea (vigna unguiculata) varieties. Afr J Biotechnol 10(43):8593–8598. doi: 10.5897/AJB10.1494 CrossRefGoogle Scholar

Copyright information

© Springer International Publishing AG 2016

Authors and Affiliations

  • C. Masso
    • 1
    Email author
  • R. W. Mukhongo
    • 2
  • M. Thuita
    • 1
  • R. Abaidoo
    • 3
  • J. Ulzen
    • 3
  • G. Kariuki
    • 4
  • M. Kalumuna
    • 5
  1. 1.International Institute of Tropical Agriculture (IITA), C/O ICIPENairobiKenya
  2. 2.Department of Agricultural Production, School of Agricultural Sciences, College of Agriculture and Environmental SciencesMakerere UniversityKampalaUganda
  3. 3.Department of Crop and Soil SciencesKwame Nkrumah University of Science and TechnologyKumasiGhana
  4. 4.Department of Biological SciencesEgerton UniversityNjoroKenya
  5. 5.Agricultural Research Institute, MlinganoTangaTanzania

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