Abstract
Antisperm antibodies (ASA) may interfere with different steps of the fertilization process. The dysfunction evolving depends on the cognate antigens of the ASA. The usual technique for their identification is 2D-Western blot and MALDI protein analysis. A great variety of antigens are responsible for sperm agglutination, sperm apoptosis and inhibition of sperm motility. Impairment of migration in the cervix mucus appears mostly to be independent from the cognate antigens of ASA, but to be a consequence complement activation and cell lysis. Influences of ASA on acrosome reaction reveal a special problem, since a large variety of cognate antigen are liberated only after acrosome reaction. The cognate antigens of ASA inhibiting zona binding and oolemma binding are the most promising structures for immunocontraception. Also pronucleus formation may be influenced by ASA, but the clinical relevance of the antigens involved remains unclear.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Auer J, Camoin L, Courtot AM, Hotellier F, De Almeida M (2004) Evidence that P36, a human sperm acrosomal antigen involved in the fertilization process is triosephosphate isomerase. Mol Reprod Dev 68(4):515–523
Bandivdekar AH, Vernekar VJ, Moodbidri SB, Koide SS (2001) Characterization of 80kDa human sperm antigen responsible for immunoinfertility. Am J Reprod Immunol 45:28–34
Bandivdekar AH, Vernekar VJ, Kamada M, Raghavan VP (2005) Antifertility effect of passive administration of antibodies to 80 kDa human sperm antigen and its synthetic peptides in male and female rats. Am J Reprod Immunol 54(6):332–341
Bastian Y, Roa-Espitia AL, Mújica A, Hernández-González EO (2010) Calpain modulates capacitation and acrosome reaction through cleavage of the spectrin cytoskeleton. Reproduction 140(5):673–684
Batova IN, Richardson RT, Widgren EE, O’Rand MG (2000) Analysis of the autoimmune epitopes on human testicular NASP using recombinant and synthetic peptides. Clin Exp Immunol 121:201–209
Bianchi E, Doe B, Goulding D, Wright GJ (2014) Juno is the egg Izumo receptor and is essential for mammalian fertilization. Nature 508(7497):483–487
Bohring C, Skrzypek J, Krause W (2001) Influence of antisperm antibodies on the acrosome reaction as determined by flow cytometry. Fertil Steril 76(2):275–280
Bohring C, Krause E, Habermann B, Krause W (2001a) Isolation and identification of sperm membrane antigens recognized by antisperm antibodies, and their possible role in immunological infertility disease. Mol Hum Reprod 7:113–118
Bronson RA, Cooper GW, Rosenfeld DL, Gilbert JV, Plaut AG (1987) the effect of an IgA1 protease on immunoglobulins bound to the sperm surface and sperm cervical mucus penetrating ability. Fertil Steril 47:985–991
Brunner-Agten S, Pavlovic R, Müller L, Horn MP, Huber AR, Stadler BM, Vogel M (2013) Increased level of antibodies cross-reacting with Ves v 5 and CRISP-2 in MAR-positive patients. Int Arch Allergy Immunol 160(1):47–55
Busso D, Cohen DJ, Hayashi M, Kasahara M, Cuasnicú PS (2005) Human testicular protein TPX1/CRISP-2: localization in spermatozoa, fate after capacitation and relevance for gamete interaction. Mol Hum Reprod 11(4):299–305
Castro A, Parodi D, Morales I, Madariaga M, Rios R, Smith R (2004) Absence of Fas protein detection by flow cytometry in human spermatozoa. Fertil Steril 81(4):1019–1025
Chan CP, Gupta S, Mark GE (1999) Identification of linear surface epitopes on the guinea pig sperm membrane protein PH-20. Life Sci 64:1989–2000
Cheng GY, Shi JL, Wang M, Hu YQ, Liu CM, Wang YF, Xu C (2007) Inhibition of mouse acrosome reaction and sperm-zona pellucida binding by anti-human sperm membrane protein 1 antibody. Asian J Androl 9(1):23–29
Chiu WW, Erikson EK, Sole CA, Shelling AN, Chamley LW (2004) SPRASA, a novel sperm protein involved in immune-mediated infertility. Hum Reprod 19(2):243–249
Clarke GN (1985) Induction of the shaking phenomenon by IgA class antispermatozoal antibodies from serum. Am J Reprod Immunol Microbiol 9:12–14
Domagala A, Pulido S, Kamieniczna M, Kurpisz M, Herr JC (2011) An isoimmune response to human sperm clathrin in an infertile woman with systemic lupus erythematosus. J Reprod Immunol 89(1):95–102
Feng HL, Han YB, Sparks AE, Sandlow JI (2008) Characterization of human sperm antigens reacting with anti-sperm antibodies from an infertile female patient’s serum. J Androl 29(4):440–448
Feng HL, Sandlow JI, Zheng LJ (2005) C-kit receptor and its possible function in human spermatozoa. Mol Reprod Dev 70(1):103–110
Francavilla F, Romano R, Santucci R (1991) Effect of sperm-antibodies on acrosome reaction of human sperm used for the hamster egg penetration assay. Am J Reprod Immunol 25:77–80
Fu J, Wang Y, Fok KL, Yang D, Qiu Y, Chan HC, Koide SS, Miao S, Wang L (2012) Anti-ACTL7a antibodies: a cause of infertility. Fertil Steril 97(5):1226–33.e1–8
Grizzi F, Chiriva-Internati M, Franceschini B, Hermonat PL, Soda G, Lim SH, Dioguardi N (2003) Immunolocalization of sperm protein 17 in human testis and ejaculated spermatozoa. J Histochem Cytochem 51(9):1245–1248
Haas GG Jr (1987) Immunologic infertility. Obstet Gynecol Clin North Am 14:1069–1085
Hamatani T, Tanabe K, Kamei K, Sakai N, Yamamoto Y, Yoshimura Y (2000) A monoclonal antibody to human SP-10 inhibits in vitro the binding of human sperm to hamster oolemma but not to human Zona pellucida. Biol Reprod 62(5):1201–1208
Hasegawa A, Shigeta M, Shibahara H (2016) Sperm immobilizing antibody and its target antigen. Immune Infertility [Epub ahead of print]
Inaba K, Morisawa S, Morisawa M (1998) Proteasomes regulate the motility of salmonid fish sperm through modulation of cAMP-dependent phosphorylation of an outer arm dynein light chain. J Cell Sci 111:1105–1115
Inoue N, Ikawa M, Isotani A, Okabe M (2005) The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs. Nature 434(7030):234–238
Jager S, Kremer J, Kuiken J, van Slochteren-Draaisma T, Mulder I, de Wilde-Janssen IW (1981) Induction of the shaking phenomenon by pretreatment of spermatozoa with sera containing antispermatozoal antibodies. Fertil Steril 36(6):784–791
Jiang H, Pillai S (1998) Complement regulatory proteins on the sperm surface: relevance to sperm motility. Am J Reprod Immunol 39:243–248
Kamieniczna M, Domagała A, Kurpisz M (2003) The frequency of antisperm antibodies in infertile couples – a Polish pilot study. Med Sci Monit 9(4):CR142–CR149
Koide SS, Wang L, Kamada M (2000) Antisperm antibodies associated with infertility: properties and encoding genes of target antigens. Proc Soc Exp Biol Med 224:123–132
Kong M, Richardson RT, Widgren EE, O’Rand MG (1995) Sequence and localization of the mouse sperm autoantigenic protein, Sp17. Biol Reprod 53(3):579–590
Kuang Y, Yan YC, Gao AW, Zhai YM, Miao SY, Wang LF, Koide SS (2000) Immune responses in rats following oral immunization with attenuated Salmonella typhimurium expressing human sperm antigen. Arch Androl 45:169–180
Kurth BE, Digilio L, Snow P, Bush LA, Wolkowicz M, Shetty J, Mandal A, Hao Z, Reddi PP, Flickinger CJ, Herr JC (2008) Immunogenicity of a multi-component recombinant human acrosomal protein vaccine in female Macaca fascicularis. J Reprod Immunol 77(2):126–141. Epub 2007 Jul 23
Lea IA, Adoyo P, O’Rand MG (1997) Autoimmunogenicity of the human sperm protein Sp17 in vasectomized men and identification of linear B cell epitopes. Fertil Steril 67(2):355–361
Lea IA, Richardson RT, Widgren EE, O’Rand MG (1996) Cloning and sequencing of cDNAs encoding the human sperm protein, Sp17. Biochim Biophys Acta 1307(3):263–266
Lea IA, Widgren EE, O’Rand MG (2004) Association of sperm protein 17 with A-kinase anchoring protein 3 in flagella. Reprod Biol Endocrinol 2:57
Li H, Ding X, Guo C, Guan H, Xiong C (2012) Immunization of male mice with B-cell epitopes in transmembrane domains of CatSper1 inhibits fertility. Fertil Steril 97(2):445–452
Li HG, Liao AH, Ding XF, Zhou H, Xiong CL (2006) The expression and significance of CATSPER1 in human testis and ejaculated spermatozoa. Asian J Androl 8(3):301–306
Liu B, Tang M, Han Z, Li J, Zhang J, Lu P, Song N, Wang Z, Yin C, Zhang W (2014) Co-incubation of human spermatozoa with anti-VDAC antibody reduced sperm motility. Cell Physiol Biochem 33(1):142–150
Liu DY, Clarke GN, Baker HW (1991) Inhibition of human sperm-zona pellucida and sperm-oolemma binding by antisperm antibodies. Fertil Steril 55:440–442
Mahony MC, Blackmore PF, Bronson RA, Alexander NJ (1991) Inhibition of human sperm-zona pellucida tight binding in the presence of antisperm antibody positive polyclonal patient sera. J Reprod Immunol 19:287–301
Margalit M, Yogev L, Yavetz H, Lehavi O, Hauser R, Botchan A, Barda S, Levitin F, Weiss M, Pastan I, Wreschner DH, Paz G, Kleiman SE (2012) Involvement of the prostate and testis expression (PATE)-like proteins in sperm-oocyte interaction. Hum Reprod 27(5):1238–1248
Menge AC, Christman GM, Ohl DA, Naz RK (1999) Fertilization antigen-1 removes antisperm autoantibodies from spermatozoa of infertile men and results in increased rates of acrosome reaction. Fertil Steril 71:256–260
Naaby-Hansen S, Herr JC (2010) Heat shock proteins on the human sperm surface. J Reprod Immunol 84(1):32–40. doi:10.1016/j.jri.2009.09.006. Epub 2009 Dec 3
Naz RK (1992) Effects of antisperm antibodies on early cleavage of fertilized ova. Biol Reprod 46:130–139
Naz RK (2008) Immunocontraceptive effect of Izumo and enhancement by combination vaccination. Mol Reprod Dev 75(2):336–344
Naz RK, Zhu X, Kadam AL (2000) Identification of human sperm peptide sequence involved in egg binding for immunocontraception. Biol Reprod 62:318–324
Neilson LI, Schneider PA, Van Deerlin PG, Kiriakidou M, Driscoll DA, Pellegrini MC, Millinder S, Yamamoto KK, French CK, Strauss JF 3rd (1999) cDNA cloning and characterization of a human sperm antigen (SPAG6) with homology to the product of the Chlamydomonas PF16 locus. Genomics 60:272–280
Norton EJ, Diekman AB, Westbrook VA, Flickinger CJ, Herr JC (2001) RASA, a recombinant single-chain variable fragment (scFv) antibody directed against the human sperm surface: implications for novel contraceptives. Hum Reprod 16:1854–1860
Paasch U, Grunewald S, Glander HJ (2002) Presence of up- and downstream caspases in relation to impairment of human spermatogenesis. Andrologia 34:279–280, abstract
Palermo GD, Colombero LT, Rosenwaks Z (1997) The human sperm centrosome is responsible for normal syngamy and early embryonic development. Rev Reprod 2:19–27
Petit FM, Serres C, Auer J (2014) Moonlighting proteins in sperm-egg interactions. Biochem Soc Trans 42(6):1740–1743
Shibahara H, Shiraishi Y, Hirano Y, Kasumi H, Koyama K, Suzuki M (2007) Relationship between level of serum sperm immobilizing antibody and its inhibitory effect on sperm migration through cervical mucus in immunologically infertile women. Am J Reprod Immunol 57(2):142–146
Tamburrino L, Marchiani S, Vicini E, Muciaccia B, Cambi M, Pellegrini S, Forti G, Muratori M, Baldi E (2015) Quantification of CatSper1 expression in human spermatozoa and relation to functional parameters. Hum Reprod 30(7):1532–1544. doi:10.1093/humrep/dev103. Epub 2015 May 15
Vazquez-Levin MH, MarÃn-Briggiler CI, Veaute C (2014) Antisperm antibodies: invaluable tools toward the identification of sperm proteins involved in fertilization. Am J Reprod Immunol 72(2):206–218
Veaute C, Furlong LI, Bronson R, Harris JD, Vazquez-Levin MH (2009) Acrosin antibodies and infertility. I. Detection of antibodies towards proacrosin/acrosin in women consulting for infertility and evaluation of their effects upon the sperm protease activities. Fertil Steril 91(4):1245–1255
Wagner A, Holland OJ, Tong M, Shelling AN, Chamley LW (2015) The role of SPRASA in female fertility. Reprod Sci 22(4):452–461
Wang M, Shi JL, Cheng GY, Hu YQ, Xu C (2009) The antibody against a nuclear autoantigenic sperm protein can result in reproductive failure. Asian J Androl 11(2):183–192
Wang Z, Zhang Y, Mandal A, Zhang J, Giles FJ, Herr JC, Lim SH (2004) The spermatozoa protein, SLLP1, is a novel cancer-testis antigen in hematologic malignancies. Clin Cancer Res 10(19):6544–6550
Wang C, Baker HW, Jennings MG, Burger HG, Lutjen P (1985) Interaction between human cervical mucus and sperm surface antibodies. Fertil Steril 44:484–488
Williams J, Samuel A, Naz RK (2008) Presence of antisperm antibodies reactive with peptide epitopes of FA-1 and YLP12 in sera of immunoinfertile women. Am J Reprod Immunol 59(6):518–524
Xiang SD, Gao Q, Wilson KL, Heyerick A, Plebanski M. Mapping T and B cell epitopes in sperm protein 17 to support the development of an ovarian cancer vaccine. Vaccine. 2015. 33(44):5950–5959. pii: S0264-410X(15)01098-1
Xu B, Copolla M, Herr JC, Timko MP (2007) Expression of a recombinant human sperm-agglutinating mini-antibody in tobacco (Nicotiana tabacum L.). Soc Reprod Fertil Suppl 63:465–477
Zhang CH, Li FQ, Yang AL, Sun W, Miao JW (2007) Detection of anti-Sp17 antibodies in infertile patients’ serum and its clinical significance. Zhonghua Nan Ke Xue 13(1):27–29
Zhang J, Ding X, Bian Z, Xia Y, Lu C, Wang S, Song L, Wang X (2010) The effect of anti-eppin antibodies on ionophore A23187-induced calcium influx and acrosome reaction of human spermatozoa. Hum Reprod 25(1):29–36
Zhang X, Ibrahim E, de Rivero Vaccari JP, Lotocki G, Aballa TC, Dietrich WD, Keane RW, Lynne CM, Brackett NL (2013) Involvement of the inflammasome in abnormal semen quality of men with spinal cord injury. Fertil Steril 99(1):118–124
Zhu X, Naz RK (1997) Fertilization antigen-1: cDNA cloning, testis-specific expression, and immunocontraceptive effects. Proc Natl Acad Sci U S A 944:704–709
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer International Publishing Switzerland
About this chapter
Cite this chapter
Krause, W.K.H. (2017). Sperm Functions Influenced by Immune Reactions. In: Krause, W., Naz, R. (eds) Immune Infertility. Springer, Cham. https://doi.org/10.1007/978-3-319-40788-3_4
Download citation
DOI: https://doi.org/10.1007/978-3-319-40788-3_4
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-40786-9
Online ISBN: 978-3-319-40788-3
eBook Packages: MedicineMedicine (R0)