Skip to main content
View expanded cover

Nanoscale Imaging and Characterisation of Amyloid-β pp 53–72Cite as

Experimental Methodology

  • 337 Accesses

Part of the Springer Theses book series (Springer Theses)

Abstract

Within this chapter are detailed descriptions of all techniques used during the work in this thesis are described. The chapter begins by discussing the SPM techniques used in this chapter as multiple modes have been used. Care is given to describe and highlight the differences in data collection from these different systems. Details of the sample preparation and any biological experimental design are noted in the latter part of the chapter. Where work was conducted by another researcher, or a contribution has been made in some manner, it is also noted here.

Keywords

  • Translational Diffusion Coefficient
  • Ultrasonic Force Microscopy
  • Scanner Tube
  • Isopropanol Alcohol
  • Anasys Instrument

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

This is a preview of subscription content, access via your institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • DOI: 10.1007/978-3-319-39534-0_4
  • Chapter length: 20 pages
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
eBook
USD   109.00
Price excludes VAT (USA)
  • ISBN: 978-3-319-39534-0
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD   139.99
Price excludes VAT (USA)
Hardcover Book
USD   139.99
Price excludes VAT (USA)
Learn about institutional subscriptions
Fig. 4.1
Fig. 4.2
Fig. 4.3
Fig. 4.4
Fig. 4.5
Fig. 4.6
Fig. 4.7
Fig. 4.8
Fig. 4.9
Fig. 4.10
Fig. 4.11
Fig. 4.12

References

  1. Digital Instruments MultiMode SPM Manual. (1999).

    Google Scholar 

  2. Yamanaka, K., Ogiso, H., & Kolosov, O. (1994). Analysis of subsurface imaging and effect of contact elasticity in the ultrasonic force microscope. Japanese Journal of Applied Physics Part 1—Regular Papers Short Notes & Review Papers, 33, 3197–3203.

    Google Scholar 

  3. Tovee, P. D., & Kolosov, O. V. (2013). Mapping nanoscale thermal transfer in-liquid environment-immersion scanning thermal microscopy. Nanotechnology, 24, 8.

    CrossRef  Google Scholar 

  4. Dobson, P.S., Weaver, J. M. R., & Mills, G. (2007). New methods for calibrated Scanning Thermal Microscopy (SThM). IEEE Sensors.

    Google Scholar 

  5. Tovee, P., Pumarol, M., Zeze, D., Kjoller, K., & Kolosov, O. (2012). Nanoscale spatial resolution probes for scanning thermal microscopy of solid state materials. Journal of Applied Physics, 112.

    Google Scholar 

  6. Mayes, J., et al. (2014). beta-amyloid fibrils in alzheimer disease are not inert when bound to copper ions but can degrade hydrogen peroxide and generate reactive oxygen species. Journal of Biological Chemistry, 289, 12052–12062.

    CrossRef  Google Scholar 

  7. Smith, A.D., et al. (2013). Near-field optical microscopy with an infra-red free electron laser applied to cancer diagnosis. Applied Physics Letters, 102.

    Google Scholar 

  8. Thompson, N. R., et al. (2012). First lasing of the ALICE infra-red free-electron laser. Nuclear Instruments & Methods in Physics Research Section a-Accelerators Spectrometers Detectors and Associated Equipment, 680, 117–123.

    ADS  CrossRef  Google Scholar 

  9. Horcas, I., et al. (2007). WSXM: A software for scanning probe microscopy and a tool for nanotechnology. Review of Scientific Instruments, 78, 013705.

    ADS  CrossRef  Google Scholar 

  10. Kiselyova, O., & Yaminsky, I. (2004). In P. Braga & D. Ricci (Eds.), Atomic force microscopy (pp. 217–230). Humana Press.

    Google Scholar 

  11. Manzoni, C., et al. (2009). Overcoming synthetic A beta peptide aging: A new approach to an age-old problem. Amyloid-Journal of Protein Folding Disorders, 16, 71–80.

    CrossRef  Google Scholar 

  12. Levine, H. (1993). Thioflavine-T interaction with synthetic alzheimers-disease beta-amyloid peptides—Detection of amyloid aggregation in solution. Protein Science, 2, 404–410.

    CrossRef  Google Scholar 

  13. Cizas, P., et al. (2010). Size-dependent neurotoxicity of beta-amyloid oligomers. Archives of Biochemistry and Biophysics, 496, 84–92.

    CrossRef  Google Scholar 

  14. Parbhu, A., Lin, H., Thimm, J., & Lal, R. (2002). Imaging real-time aggregation of amyloid beta protein (1-42) by atomic force microscopy. Peptides, 23, 1265–1270.

    CrossRef  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physics, Lancaster University, Lancaster, UK

    Claire Louisa Tinker-Mill

Authors
  1. Claire Louisa Tinker-Mill
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Claire Louisa Tinker-Mill .

Rights and permissions

Reprints and Permissions

Copyright information

© 2016 Springer International Publishing Switzerland

About this chapter

Cite this chapter

Tinker-Mill, C.L. (2016). Experimental Methodology. In: Nanoscale Imaging and Characterisation of Amyloid-β. Springer Theses. Springer, Cham. https://doi.org/10.1007/978-3-319-39534-0_4

Download citation