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Genome Editing pp 65–77Cite as

CRISPR/Cas9 and the Paradigm Shift in Mouse Genome Manipulation Technologies

Abstract

The CRISPR revolution that began in 2013 has been adopted and embraced by many researchers worldwide, including the mouse molecular genetics community. CRISPR represents one of only a few radical and transformative shifts in transgenic technologies over the past 30 years. This chapter discusses the paradigm shift that CRISPR technology has brought about in the field of mouse genome editing.

Keywords

  • Transgenic
  • Knockout
  • Knock-in
  • Genome editing
  • CRISPR/Cas9
  • Mouse genome
  • Microinjection

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Acknowledgments

C.B.G. acknowledges funding support by the NIH grants: Institutional Development Award (IDeA) from the National Institutes for General Medical Sciences grant number P20GM103471 (NIGMS) and NIH Office of Research Infrastructure Programs (ORIP) grant number R24OD018546 (ORIP/DPCPSI). M.O. acknowledges the funding support by the 2014 Tokai University School of Medicine Research Aid and Grant-in-Aid for challenging Exploratory Research (15 K14371) from Japan Society for the Promotion of Science (JSPS).

Author information

Authors and Affiliations

  1. Developmental Neuroscience, Munroe Meyer Institute for Genetics and Rehabilitation, University of Nebraska Medical Center, Omaha, NE, USA

    Channabasavaiah B. Gurumurthy

  2. Mouse Genome Engineering Core Facility, Office of the Vice Chancellor for Research, University of Nebraska Medical Center, Omaha, NE, USA

    Channabasavaiah B. Gurumurthy & Rolen M. Quadros

  3. Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University, Kagoshima, 890-8544, Japan

    Masahiro Sato

  4. Institute of Experimental Animal Sciences, Graduate School of Medicine, Osaka University, Osaka, 565-0871, Japan

    Tomoji Mashimo

  5. Mouse Biology Program, University of California, Davis, 2795 2nd Street, Davis, CA, 95618, USA

    K. C. Kent Lloyd

  6. Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan

    Masato Ohtsuka

Authors
  1. Channabasavaiah B. Gurumurthy
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  2. Rolen M. Quadros
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  3. Masahiro Sato
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  4. Tomoji Mashimo
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  5. K. C. Kent Lloyd
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  6. Masato Ohtsuka
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Corresponding authors

Correspondence to Channabasavaiah B. Gurumurthy or Masato Ohtsuka .

Editor information

Editors and Affiliations

  1. Ottawa, Ontario, Canada

    Kursad Turksen

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© 2016 Springer International Publishing Switzerland

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Gurumurthy, C.B., Quadros, R.M., Sato, M., Mashimo, T., Lloyd, K.C.K., Ohtsuka, M. (2016). CRISPR/Cas9 and the Paradigm Shift in Mouse Genome Manipulation Technologies. In: Turksen, K. (eds) Genome Editing. Springer, Cham. https://doi.org/10.1007/978-3-319-34148-4_4

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