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Molecular Diversity and Detection of Endophytic Fungi Based on Their Antimicrobial Biosynthetic Genes

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Molecular Markers in Mycology

Part of the book series: Fungal Biology ((FUNGBIO))

Abstract

Endophytic fungi are important component of plant microbiota which ubiquitously resides in the plant tissues at least for a part of their life cycle without causing any harm to the host. Endophytic fungi have been reported from wide variety of host plants from different ecosystems However many endophytic fungi do not sporulate and use of morphology based identification approach limits their identification. To overcome this difficulty, DNA based molecular markers and metagenomics using next generation sequencing (NGS), have successfully employed in genetic diversity estimation and community analysis of endophytic fungi. Endophytic fungi synthesizes plethora of antimicrobial secondary metabolites which plays key role in protecting the host against various biotic stresses including alkaloids, polyketides, aliphatic compounds, peptides etc. Polyketide synthase (PKS) and non ribosomal peptide synthases (NRPS) encoded by PKS gene and NRPS gene respectively biosynthesize polyketides and oligopeptide secondary metabolites, which have been reported to associate with antimicrobial activity of endophytic fungi. This chapter summarises approaches employed in detection and analysis of diversity of endophytic fungi as well as detection of antimicrobial genes of endophytic fungi by using molecular tools.

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Acknowledgements

BPS is thankful to University Grant Commission (UGC) for financial support as major research project. VKM is thankful to Department of Science and Technology (DST), New Delhi for providing fellowship under DST INSPIRE fellowship (IF 130374). Authors are also thankful to the Department of Biotechnology, New Delhi for establishment of DBT-BIF centre and DBT-State Biotech hub in the Department of Biotechnology, Mizoram University which has been used for the present study.

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  1. Molecular Microbiology and Systematic Laboratory, Department of Biotechnology, Mizoram University, Aizawl, Mizoram, 796004, India

    Vineet Kumar Mishra, Ajit Kumar Passari, Vincent Vineeth Leo & Bhim Pratap Singh

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Correspondence to Bhim Pratap Singh .

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  1. Department of Biotechnology, Mizoram University, Aizawl, Mizoram, India

    Bhim Pratap Singh

  2. Department of Biochemistry, National University of Ireland Galway, Galway, Ireland

    Vijai Kumar Gupta

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Mishra, V.K., Passari, A.K., Leo, V.V., Singh, B.P. (2017). Molecular Diversity and Detection of Endophytic Fungi Based on Their Antimicrobial Biosynthetic Genes. In: Singh, B.P., Gupta, V.K. (eds) Molecular Markers in Mycology. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-34106-4_1

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