Abstract
The method of electron spin resonance (ESR, Sect. 3.1) has proven to be the leading approach in the investigation of electron spin interactions in chemistry, biology and physics. Structural and dynamic properties of molecular objects of interest can be measured and characterized by suitable parameters through an arsenal of experimental ESR methods including conventional Continuous wave (CW ESR), electrically (ED ESR) and optically (OD ESR) detected ESR and advanced pulse techniques. The CW technique is used for collecting information of interest through the analysis of ESR spectra or saturation curves, obtained from electron-electron double resonance (ELDOR) or double electron-electron resonance (DEER), electron-nuclear double resonance (ENDOR ) or multifrequency ESR (MF ESR) techniques. OD ESR makes it possible to acquire good ESR, ELDOR and ENDOR spectra using the simple and very sensitive luminescence technique. Application of such methods as two-dimensional ESR (2D ESR), (fried) two-dimensional electron-electron double resonance (2D-ELDOR ), ENDOR with circularly polarized radiofrequency fields (CP-ENDOR), electron-nuclear-nuclear resonance (double ENDOR), proton-electron double resonance imaging (PEDRI), and electron-nuclear-nuclear triple resonance (TRIPLE) can be more informative than simple CW ESR experiments for appropriate systems. Methods of reaction yield detection magnetic resonance (RYDMR) and magnetically affected reaction yield (MARY) can also be used in the context of CW and pulse techniques. This chapter briefly describes theoretical grounds and typical application of the various methods of modern CW ESR spectroscopy, which continue to evolve synergetic and at a rather rapid pace.
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References
A.J. Hoff (ed.), Advanced EPR. Applications in Biology and Biochemistry (Elsevier, Amsterdam, 1989)
C.P. Poole, Electron Spin Resonance: A Comprehensive Treatise on Experimental Techniques (Dover Publcation, 1997)
G.R. Eaton, K.M. Salikhov, S.S. Eaton, Foundations of Modern EPR (World Scientific, Singapore, 1998)
J. Yamauchi, in Likhtenshtein GI, Yamauchi J, Nakatsuji S, Smirnov A., Tamura R. Nitroxides: Application in Chemistry, Biomedicine, and Materials Science (WILEY-VCH, Weinhem, 2008), p. 71
A.I. Smirnov, in Likhtenshtein GI, Yamauchi J, Nakatsuji S, Smirnov A., Tamura R. Nitroxides: Application in Chemistry, Biomedicine, and Materials Science (WILEY-VCH, Weinhem, 2008), p. 121
M.R. Brustolon, Principles and Applications of Electron Paramagnetic Resonance Spectroscopy (Blackwell Publishers, 2008)
W.R Hagen, Biomolecular EPR Spectroscopy (CRC, 2008)
Savitsky A, Mobius K High-Field EPR Spectroscopy on Proteins and Their Model Systems (Wiley-VCH Verlag GmbH, 2009)
G.R. Eaton, S.S. Eaton, D.P. Barr, R.T. Weber, Quantitative EPR (Springer, 2010)
B.C. Gilbert, M.J. Davies, K.A. McLauchlan (eds.), Electron Paramagnetic Resonance, a Specialist Periodical Report, vol. 17 (Royal Society of Chemistry, 2010)
G.M. Smith, J. Keeble, O. Schiemann (eds.), Introduction to Modern EPR Spectroscopy (CRC, 2010)
A. Belay, Fluorescence and Electron Paramagnetic Resonance (EPR) Spectroscopy (LAP LAMBERT Academic Publishing, 2011)
S.K. Misra (ed.), Multifrequency Electron Paramagnetic Resonance (Wiley-VCH, New York, 2011)
G.I. Likhtenshtein, Biophysical Labeling Methods in Molecular Biology (Cambridge University Press. Cambridge, NY, 1993)
A. Brunetti, O. Baffa, Radiat. Meas. 32,361 (2000)
E. Husted, A. Beth, in Magnetic Resonance in Biology, vol. 19, ed. by L. Berliner, S. Eaton, G. Eaton (Kluwer Academic Publishers, Dordrecht 2000), p. 155
W. Xiao, Y.-K. Shin, in Magnetic Resonance in Biology, vol. 19, ed. by L. Berliner, S. Eaton, G. Eaton (Kluwer Academic Publishers, Dordrecht 2000), p. 249
M. Portis, Phys. Rev. 91, 1071 (1953)
T.G. Jr, Castner. Phys. Rev. 115, 1506 (1959)
Ya. S, Lebedev, and V.I. Muromtsev, ESR and Relaxation of Stable Radicals (Khimiya, Moscow, 1972)
A.V. Kulikov, G.I. Likhtenstein, Biofizika 19, 420 (1974)
A.V. Kulikov, G.I. Likhtenstein, Adv. Molecul. Relax. Proc. 10, 47 (1977)
G.I. Likhtenstein, in Magnetic Resonance in Biology, vol. 19, ed. by L. Berliner, S. Eaton, G. Eaton (Kluwer Academic Publishers, Dordrecht 2000), p. 309
A. Lund, E. Sagstuen, A. Sanderud, J. Maruani, Radiat. Res. 172, 753 (2009)
G.I. Likhtenshtein, A.V. Kulikov, A.I. Kotelnikov, V.R. Bogatyrenko, Photobiochem. Photobiol. 3, 178 (1982)
A.V. Kulikov, Mol. Biol. (Moscow) 10, 109 (1976)
K.M. Salikhov, J. Magn. Reson. 63, 241 (1985)
Shojiro Kimura, Yuya Sawada, Yasuo Narumi, Kazuo Watanabe, Masayuki Hagiwara, Koichi Kindo, Hiroaki Ueda, Phys. Rev. B 92, 144410 (2015)
K.I. Zamaraev, Yu.N. Molin, K.M. Salikhov, Spin Exchange. Theory and Physicochemical Application (Springer-Verlag. Heidelberg, 1981)
G.I. Likhtenstein, Y.B. Grebentchikov, P.K. Bobodzhanov, Y.V. Kokhanov, Molec. Biol. (Moscow) 4, 782 (1970)
J.S. Hyde, H.M Swartz, W.E. Antholine, The spin probe-spin label methods, in Spin Labeling. Theory and Application, vol. 2, ed. by L. Berliner (Academic Press, New York, 1976), p. 72
C. Altenbach, W. Froncisz, J.S. Hyde, W.L. Hubbell, Biophys. J. 56, 61183 (1989)
K.M. Salikhov, A.B. Doctorov, YuN Molin, K.I. Zamaraev, Spin relaxation of radicals and complexes upon encounters in solution. J. Magnet. Reson. 5, 189 (1971)
V.N. Parmon, A.I. Kokorin, G.M. Zhidomirov, Stable Biradicals (Nauka, Moscow, 1980)
G.R. Eaton S. S. Eaton, Biol. Magn. Reson. 8, 339 (1989)
S. Ngo, V.V. Chiang, Z. Guo, J. Struct. Biol. 180, 374 (2012)
V. Paredes-García, R.C. Santana, R. Madrid, B. Baldo, A. Vega, E. Spodine, J. Inorg. Biochem. 114, 75 (2012)
H.S. Mchaourab, S. Pfenninger, W.E. Antholine, W.E. Antholine, C.C. Felix, J.S. Hyde, P.M. Kroneck, Biophys. J. 64, 1576 (1993)
C.S. Klug, T.G. Camenisch, W.L. Hubbell, J.S. Hyde, Biophys. J. 88, 3641 (2005)
E.J. Hustedt, A.H. Beth, Ann. Rev. Biophys. Biomol. Struc. 28, 129 (1999)
C. Riplinger, J.P.Y. Kao, G.M. Rosen, V. Kathirvelu, G.R. Eaton, S.S. Eaton, A. Kutateladze, F. Neese, J. Am. Chem. Soc. 131, 10092 (2009)
S.K Misra, J.H. Freed, in Multifrequency Electron Paramagnetic Resonance, ed. by S.K Misra (Wiley-VCH, New York, 2011), p. 545
A.W. Kittell, E.J. Hustedt, J.S. Hyde, J. Magn. Res. 221, 51 (2012)
A. Berliner., S. Eaton, G. Eaton (eds.) Magnetic Resonance in Biology. Distance Measurement in Biological Systems by ESR, vol. 19 (Kluwer Academic Publishers, Dordrecht, 2000)
O.Y. Grinberg, A.A. Dubinskii, V.F. Shuvalov, L.G. Oranskii, V.I. Kurochkin, YaS Lebedev, Dokl. Akad. Nauk SSSR 230, 884 (1976)
Ya. S. Lebedev, Appl. Magn. Res. 7, 339 (1994)
V.I. Krinichnyi, 2-mm Wave Band EPR Spectroscopy of Condensed Systems (Boca Raton, CRC Press, 1995)
O.Y. Grinberg, L.J. Berliner (eds.) Very High Frequency (VHF) ESR/EPR, vol. 22. Series Biological Magnetic Resonance (Kluwer Academic Plenum Publishers, 2004)
V.I. Krinichnyi, in Spectroscopy of Polymer Nanocomposites, ed. by D. Ponnamma, D. Rouxel, S. Thomas (Elsevier, 2015), p. 202
D.E. Budil, K.A. Earle, W.B. Lynch, J.H. Freed, in Advanced ESR: Application in Biology and Biochemistry, ed. by A.J. Hoff (Elsevier, Amsterdam, 1989)
J.H. Freed, Ann. Rev. Phys. Chem. 51, 655 (2000)
D. Kivelson, J. Chem. Phys. 33, 1094 (1960)
J.H. Freed, in Spin Labeling. Theory and Applications, vol. 1, ed. by L. Berliner (Academic Press, New York, 1976)
A. Polimeno, V. Barone, J.H. Freed, in Computational Spectrocopy, Chapter 12, ed. by V. Barone (Wiley, New York, NY, 2012)
S.K. Misra, J.H. Freed, Molecular motions, in Multifrequency Electron Paramagnetic Resonance, ed. by S.K Misra (Wiley-VCH, New York, 2011), p. 497
Z. Zhang, M.R. Fleissner, Z. Liang, D.S. Tipikin, K. Moscicki, K.A. Earle, W.L. Hubbell, J.H. Freed, J. Phys. Chem. B 114, 5503 (2010)
Y. Sun, Z. Zhang, V.M. Grigoryants, W.K. Myers, F. Liu, K.A. Earle, J.H. Freed, C.P. Scholes, Biochemistry 51, 8530 (2012)
M.D. Smigel, L.R. Dalton, J.S. Hyde, J.S. Dalton, Proc. Nat. Acad. Sci. USA 71, 1925 (1974)
A. Kusumi, J.S. Hyde, Biochemistry 21, 5978 (1982)
P. Fajer, D.D. Thomas, J.B. Feix, J.S. Hyde, Biophys. J. 50, 1195 (1986)
E.J. Hustedt, A. Beth, Biophys. J. 86, 3940 (2004)
A.H. Beth, Biophys. J. 103, 1109 (2012)
G.G. Lazarev, Ya.S. Lebedev, Khimicheskaya Fizika 7, 1159 (1988)
G. Feher. Phys. Rev. 834 (1956)
J.S. Hyde, J. Chem. Phys. 43, 1806 (1965)
H. Kurreck, B. Kirste, W. Lubitz, Electron Nuclear Double Resonance Spectroscopy of Radicals in Solution (VCH Publishers, New York, 1988)
D.M. Murphy, R.D. Farley, Chem. Soc. Rev. 35, 249 (2006)
C.E Tait,. P. Neuhaus, H.L. Anderson, C. R. Timmel, J. Am. Chem. Soc. 137, 6670 (2015)
B. Katterle, R.I. Gvozdev, N. Abudu, T. Ljones, K.K. Andersson, Biochem. J. 363, 677 (2002)
L. Kulik, W. Lubitz, Photosynth. Res. 102, 391 (2009)
J.S. Hyde, J.C.W. Chien, J.H. Freed, J. Chem. Phys. 48, 4211 (1968)
V.A. Benderskii, L.A. Blyumenfel’d, P.A. Stunzhas, E.A. Sokolov, Nature 220, 365 (1968)
J.J. Yin, J.B. Feix, J.S. Hyde, Biophys. J. 52, 1031 (1987)
L.D. Kispert, Biol. Magn. Res. (Biomedical EPR, Part B) 24, 165 (2005)
E. Reijerse, F. Lendzian, R. Isaacson, W. Lubitz, J. Magn. Res. 214, 237 (2012)
A.L. Buchachenko, Chemical Nuclear and Electron Polarization (Nauka, Moscow, 1974). (in Russian)
R.W. Field, R.S. Bradford, D.O. Harris, H.P. Broida, J. Chem. Phys. 56, 4712–4714 (1972)
O.A. Anisimov, V.M. Grigoryants, V.K. Molchanov, YuN Molin, Chem. Phys. Lett. 66, 265 (1979)
K.M. Salikhov, Y. Sakaguchi, H. Hayashi, Chem. Phys. 220, 355 (1997)
YuN Molin, O.A. Anisimov, V.M. Grigoryants, V.K. Molchanov, K.M. Salikhov, J. Phys. Chem. 84, 1853 (1980)
E.L. Frankevich, S.I. Kubarev, Triplet State Optical Detection Magnetic Resonance Spectroscopy, ed. by R.H. Clarke (John Wiley & Sons, Inc., New York, 1982)
O.A. Anisimov, V.N. Verkhovlyuk, S.B. Zikirin, A.G. Matveeva, S.I. Trashkeev, Yu.N. Molin, Appl. Magn. Reson. 45, 881 (2014)
M. Vyushkova, P. Potashov, V. Borovkov, V. Bagryansky, Y.N. Molin, in Selectivity, Control, and Fine Tuning in High-Energy Chemistry, ed. by D.V. Stass, V.I. Feldman (2011), p. 191
A. Dreau, M. Lesik, L. Rondin, P. Spinicelli, O. Arcizet, J.-F. Roch, V. Jacques, Phys. Rev. B: Condens. Matter Mater. Phys. 84, 195204/1 (2011)
E.L. Frankevich, A.I. Pristupa, V.I. Lesin, Chem. Phys. Lett. 54, 99 (1978)
Masaharu Okazaki, Takesui Shiga, Product yield of magnetic-field-dependent photochemical reaction modulated by electron spin resonance. Nature 323, 240–243 (1986)
T. Miura, A. Kageyama, S. Torii, H. Murai, J. Phys. Chem. B 114, 14550 (2010)
C.J. Wedge, J.C.S. Lau, K.-A. Ferguson, S.A. Norman, P.J. Hore, C.R. Timmel, Phys. Chem. Chem. Phys. 15, 16043 (2013)
S.N. Batchelor, K.A. McLauchlan, I.A. Shkrob, Time-resolved reaction yield detected magnetic resonance (RYDMR). Chem. Phys. Lett. 181, 327 (1991)
C.A. Hamilton, J.P. Hewitt, K.A. McLauchlan, U.E Steiner, Mol. Phys. 65,423 (1988)
V.N. Verkhovlyuk, N.N. Lukzen, J.B. Pedersen, D.V. Stass, Y.N. Molin, Dokl. Phys. Chem. 417, 311 (2007)
K.B. Henbest, E. Athanassiades, K. Maeda, I. Kuprov, P.J. Hore, C.R. Timmel, Molec. Phys. 104, 1789 (2006)
V.N. Verkhovlyuk, N.N. Lukzen, J.B. Pedersen, D.V. Stass, Y.N. Molin, Dokl. Phys. Chem. 417, 311 (2007) (Pleiades Publishing, Ltd)
K.L. Ivanov, D.V. Stass, E.V. Kalneus, R. Kaptein, N.N. Lukzen, Appl. Magn. Reson. 44, 217 (2013)
N.L. Lavrik, YuN Molin, Int. J. Quan. Chem. 40, 387 (1991)
P.A. Potashov, L.N. Shchegoleva, N.A. Gritsan, V.A. Bagryansky, Y.N. Molin, J. Phys. Chem. A 116, 3110 (2012)
V.A. Bagryansky, V.I. Borovkov, Y.N. Molin, Phys. Chem. Chem. Phys. 6, 924 (2004)
M.E. Michel-Beyerle, H.W. Krüger, R. Haberkorn, H. Seidlitz, Chem. Phys. 42, 441 (1979)
Y.N. Molin, Mendeleev Commun. 3, 85 (2004)
J. Klein, R. Voltz, Can. J. Chem. 55, 2103 (1977)
O.A. Anisimov, V.L. Bizyaev, N.N. Lukzen, V.M. Grigoryants, YuN Molin, Chem. Phys. Lett. 101, 131 (1983)
Y. Kitahama, Y. Sakaguchi, J. Phys. Chem. A 112, 176 (2008)
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Likhtenshtein, G. (2016). Experimental Methods of Investigation of Electron Spin Interactions Based on ESR Phenomena: Continuous Wave EPR Measurements. In: Electron Spin Interactions in Chemistry and Biology. Biological and Medical Physics, Biomedical Engineering. Springer, Cham. https://doi.org/10.1007/978-3-319-33927-6_5
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