Skip to main content
SpringerLink
Log in

Somatic Embryogenesis in Capsicum spp.

  • Chapter
  • First Online:
Somatic Embryogenesis: Fundamental Aspects and Applications

Abstract

Capsicum species represent a horticultural crop of great importance worldwide, which is frequently exposed to different adverse environmental abiotic or biotic factors that affect field yields. Biotechnology merges as an alternative to improve agriculturally important characteristics in Capsicum through modern approaches. Tissue culture together with molecular biology techniques is the foundation of plant biotechnology. Efficient in vitro plant regeneration protocols need to be developed and established to provide appropriate systems for micropropagation and manipulation through genetic engineering for modern crop improvement programs. Organogenesis and somatic embryogenesis are the two ways of generating entire plants in vitro. Somatic embryogenesis, the asexual process of producing bipolar structures that resemble the sexually generated zygotic embryos, has been described for a number of plant species; however, in the case of Capsicum species the in vitro embryogenesis systems have faced recalcitrancy problems. In this chapter, the information on somatic embryogenesis in Capsicum is presented, and the recalcitrancy problems are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 219.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 279.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 279.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  • Avilés-Viñas SA, Lecona-Guzmán CA, Canto-Flick A et al (2013) Morpho-histological and ultrastructural study on direct somatic embryogenesis of Capsicum chinense Jacq. in liquid medium. Plant Biotechnol Rep 7:277–286. doi:10.1007/s11816-012-0261-0

    Article  Google Scholar 

  • Binzel ML, Sankhla N, Sangeeta J, Sankhla D (1996) Induction of direct somatic embryogenesis and plant regeneration in pepper (Capsicum annuum L.). Plant Cell Rep 15:536–540. doi:10.1007/BF00232989

    Article  CAS  PubMed  Google Scholar 

  • Bodhipadma K, Leung DWM (2002) Factors important for somatic embryogenesis in zygotic embryo explants of Capsicum annuum L. J Plant Biol 45:49–55. doi:10.1007/BF03030432

    Article  Google Scholar 

  • Buyukalaca S, Mavituna F (1996) Somatic embryogenesis and plant regeneration of pepper in liquid media. Plant Cell Tiss Org Cult 46:227–235. doi:10.1007/BF02307099

    Article  CAS  Google Scholar 

  • Jo J-Y, Choi E-Y, Choi D, Lee K-W (1996) Somatic embryogenesis and plant regeneration from immature zygotic embryo culture in pepper (Capsicum annuum L.). J Plant Biol 39:127–135

    Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158. doi:10.1016/0014-4827(68)90403-5

    Article  CAS  PubMed  Google Scholar 

  • Greer MS, Kavalchuk I, Eudes F (2009) Ammonium nitrate improves direct somatic embryogenesis and biolistic transformation of Triticum aestivum. New Biotechnol 26:44–52. doi:10.1016/j.nbt.2009.02.003

    Article  CAS  Google Scholar 

  • Grimes HD, Hodges TK (1990) The inorganic NO3 −:NH4 + ratio influence plant regeneration and auxin sensitivity in primary callus derived from immature embryos of indica rice (Oryza sativa L.). J Plant Physiol 136:362–367. doi:10.1016/S0176-1617(11)80063-5

    Article  CAS  Google Scholar 

  • Harini I, Lakshmi Sita G (1993) Direct somatic embryogenesis and plant regeneration from immature embryos of chilli (Capsicum annuum L.). Plant Sci 89:107–112. doi:10.1016/0168-9452(93)90176-Z

    Article  Google Scholar 

  • Heidmann I, de Lange B, Lambalk J et al (2011) Efficient sweet pepper transformation mediated by the BABY BOOM transcription factor. Plant Cell Rep 30:1107–1115. doi:10.1007/s00299-011-1018-x

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kaparakis G, Alderson PG (2008) Role for cytokinins in somatic embryogenesis of pepper (Capsicum annuum L.). J Plant Growth Reg 27:110–114. doi:10.1007/s00344-007-9037-0

    Article  CAS  Google Scholar 

  • Khan H, Siddique I, Anis M (2006) Thidiazuron induced somatic embryogenesis and plant regeneration in Capsicum annuum. Biol Plant 50:789–792. doi:10.1007/s10535-006-0133-y

    Article  CAS  Google Scholar 

  • Kintzios S, Drossopoulos JB, Lymperopoulos Ch (2001) Effect of vitamins and inorganic micronutrients on callus growth and somatic embryogenesis from leaves of chilli pepper. Plant Cell Tiss Org 67:55–62. doi:10.1023/A:1011610413177

    Article  CAS  Google Scholar 

  • Kintzios S, Drossopoulos JB, Shortsianitis E, Peppes D (2000) Induction of somatic embryogenesis from young, fully expanded leaves of chilli pepper (Capsicum annuum L.): effect of leaf position, illumination and explant pretreatment with high cytokinin concentrations. Sci Hortic 85:137–144. doi:10.1016/S0304-4238(99)00135-1

    Article  CAS  Google Scholar 

  • Kothari SL, Joshi A, Kachhwaha S, Ochoa-Alejo N (2010) Chilli peppers - A review on tissue culture and transgenesis. Biotechnol Rev 28:35–48

    Google Scholar 

  • López-Puc G, Canto-Flick A, Barredo-Pool F et al (2006) Direct somatic embryogenesis: A highly efficient protocol for in vitro regeneration of Habanero (Capsicum chinense Jacq.). HortScience 41:1645–1650

    Google Scholar 

  • Malabadi RB, Staden JV (2006) Cold enhanced somatic embryogenesis in Pinus patula is mediated by calcium. S Afr J Bot 72:613–618. doi:10.1016/j.sajb.2006.04.001

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497. doi:10.1111/j.1399-3054.1962.tb08052.x

    Article  CAS  Google Scholar 

  • Ochoa-Alejo N, Ramírez-Malagón R (2001) In vitro chili pepper biotechnology. In Vitro Cell Dev Biol-Plant 37:701–729

    Google Scholar 

  • Pullman GS, Chopra R, Chase K-M (2006) Loblolly pine (Pinus taeda L.) somatic embryogenesis: Improvements in embryogeneic tissue initiation by supplementation of medium with organic acids, vitamins B12 and E. Plant Sci 170:648–658. doi:10.1016/j.plantsci.2005.10.018

    Article  CAS  Google Scholar 

  • Pullman GS, Chase K-M, Skryabina A, Bucalo K (2009) Conifer embryogenic tissue initiation: Improvements by supplementation of medium with D-xylose and D-chiro-inositol. Tree Physiol 29:147–156. doi:10.1093/treephys/tpn013

    Article  CAS  PubMed  Google Scholar 

  • Pullman GS, Zeng X, Copeland-Kamp B et al (2015) Conifer somatic embryogenesis: improvements by supplementation of medium with oxidation-reduction agents. Tree Physiol 35:209–224. doi:10.1093/treephys/tpu117

    Article  PubMed  Google Scholar 

  • Santana-Buzzy N, López-Puc G, Canto-Flick A et al (2009) Ontogenesis of the somatic embryogenesis of Habanero pepper (Capsicum chinense Jacq.). HortScience 44:113–118

    Google Scholar 

  • Solís-Ramos LY, Nahuath-Dzib S, Andrade-de-Torres A et al (2010) Indirect somatic embryogenesis and morphological analysis in Capsicum chinense. Biologia 65:504–511. doi:10.2478/s11756-010-0049-z

    Article  CAS  Google Scholar 

  • Steinitz B, Küsek M, Tabib Y et al (2003) Pepper (Capsicum annuum L.) regenerants obtained by direct somatic embryogenesis fail to develop a shoot. In Vitro Cell Dev-Pl 39:296–303. doi:10.1079/IVP2002405

    Article  CAS  Google Scholar 

  • Valle-Gough RE, Avilés-Viñas SA, López-Erosa S et al (2015) Polyamines and WOX genes in the recalcitrance to plant conversion of somatic embryos of Habanero pepper (Capsicum chinense Jacq.). Afr J Biotechnol 14:569–581. doi:10.5897/AJB2014.14313

    Article  CAS  Google Scholar 

  • Vieira LN, Santa-Catarina C, de FreitasFraga HP et al (2012) Glutathione improves early somatic embryogenesis in Araucaria angustifolia (Bert) O. Kuntze by alteration in nitric oxide emission. Plant Sci 195:80–87. doi:10.1016/j.plantsci.2012.06.011

    Article  CAS  Google Scholar 

  • Zapata-Castillo PY, Canto-Flick A, López-Puc G et al (2007) Somatic embryogenesis in Habanero pepper (Capsicum chinense Jacq.) from cell suspensions. HortScience 42:329–333

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Ingeniería Genética, and Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Unidad Irapuato, Km 9.6 libramiento norte carretera Irapuato-León, 36821, Irapuato, Guanajuato, Mexico

    Neftalí Ochoa-Alejo

Authors
  1. Neftalí Ochoa-Alejo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Neftalí Ochoa-Alejo .

Editor information

Editors and Affiliations

  1. Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, Mexico

    Víctor M Loyola-Vargas

  2. Departamento de Ingeniería Genética de Plantas, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Irapuato, Mexico

    Neftalí Ochoa-Alejo

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer International Publishing Switzerland

About this chapter

Cite this chapter

Ochoa-Alejo, N. (2016). Somatic Embryogenesis in Capsicum spp.. In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Somatic Embryogenesis: Fundamental Aspects and Applications. Springer, Cham. https://doi.org/10.1007/978-3-319-33705-0_14

Download citation

Publish with us

Policies and ethics

Search

Navigation