DSC-Differentiated Hepatocytes for Treatment of Liver Diseases

  • Francesco Paduano
  • Massimo Marrelli
  • Akhilesh K. Gaharwar
  • Marco TatulloEmail author
Part of the Stem Cell Biology and Regenerative Medicine book series (STEMCELL)


Dental stem cells (DSCs) can generate hepatic-like cells and represent a promising therapeutic approach for the treatment of patients suffering from liver disease such as cirrhosis and acute hepatic injury (AHF). A range of studies showed successful differentiation of DSCs into hepatocyte-like cells, demonstrating that the dental stem/progenitor cells of dental tissue. The progenitor cells isolated from dental pulp (DPSCs) and exfoliated deciduous teeth (SHEDs), under specific stimuli, are able to express hepatic specific markers, and most importantly, can engraft functionally into murine and rat models of liver injury. Furthermore, other reports demonstrated that DSCs can be reprogrammed into induced pluripotent stem cells (DP-iPSCs), and then successfully differentiated into hepatocyte-like cells (DP-iPSC-Heps) having characteristics of mature hepatocytes. The engraftment of DP-iPSC-Heps in immunocompromised AHF mouse model can be improved by the additional use of scaffolds that release hepatocyte growth factor (HGF) or liver-specific microRNA122 (miR122). This chapter shows that DSCs are a good candidate to generate hepatocyte-like cells and dental pulp-derived iPSCs-differentiated hepatocytes (DP-iPSCs-Heps) and demonstrates that they could be considered an optimal cell source for liver cell therapy.


Dental stem cells (DSCs) Hepatocyte-like cells Hepatic differentiation 



Adipose stem cells


Acute hepatic failure




Acute liver failure


Alanine aminotransferase




Bone marrow stem cells


Carboxymethyl-hexanol chitosan


miR122 complexed with PU-PEI in nanostructured CHC


Cytokeratin 18


Carbamoyl phosphate synthetase




Hepatocytes-like cells from dental pulp derived-iPSCs


Dental pulp-derived iPSC


Dental pulp stem cells


Dental stem cells


Deciduous tooth pulp cells


Epidermal growth factor


Embryonyc stem cell-derived hepatocytes


Embryonic stem cells


Embryotrophic factor


Fetal bovine serum


Fibroblast growth factor 1


Hepatocellular carcinoma


Hepatitis C virus


Human hepatically differentiated SHED


Hepatocyte growth factor


Tooth-pulp stem cells


Interleukin 6


iPSCs-derived hepatocyte-like cells


Induced pluripotent stem cells


Insulin-transferrin selenium


Multipotent adult progenitor cells




Oncostatin M


Polyurethane-graft-short-branch polyethylenimine copolymer


Exfoliated deciduous teeth stem cells


Signal transducer and activator of transcription 3




Tooth germ progenitor cells


Wharton’s jelly-derived MSCs


Wisdom tooth pulp cells




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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Francesco Paduano
    • 1
  • Massimo Marrelli
    • 2
  • Akhilesh K. Gaharwar
    • 3
    • 4
  • Marco Tatullo
    • 1
    Email author
  1. 1.Research and Development in Biomedicine – Stem Cells UnitTECNOLOGICA Research InstituteCrotoneItaly
  2. 2.Department of Maxillofacial SurgeryCalabrodentalCrotoneItaly
  3. 3.Department of Biomedical EngineeringTexas A&M UniversityCollege StationUSA
  4. 4.Department of Materials Science and EngineeringTexas A&M UniversityCollege StationUSA

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