Pruritus pp 49-55 | Cite as

In Vitro Models of Itch

  • Nicolas LebonvalletEmail author
  • Laurent Misery


Although itch is perceived through the brain, in vitro studies are possible and necessary to study its genesis. Cultures of keratinocytes or neurons or mast cells can be used. However, best models are provided by the co-culture of neurons and other cells: keratinocytes, mast cells, Langerhans cells, etc.… The best model is the co-culture of neurons and skin explants. In these models, representative biochemical, and sometimes electrophysiological, measurements can be performed.


Pruritus Itch Model In vitro Ex vivo Pruritus Histamine Neuropeptide Proteases Neuron Keratinocyte Mast cell Cell culture 



Agonist Peptide


Calcitonin Gene-Related Peptide


Central Nervous System


Dorsal Root Gangli


Matrix Metallo Protease


Nerve Growth Factor


Protease Activated Receptor


Primary Sensory Neuron


Semaphorin 3A


Substance P


Thymic Stromal Lymphopoietin


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Copyright information

© Springer-Verlag London 2016

Authors and Affiliations

  1. 1.Laboratory of Neurosciences of BrestUniversity of BrestBrestFrance
  2. 2.Laboratory of Neurosciences of Brest, Department of DermatologyUniversity Hospital, University of BrestBrestFrance

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