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Mass Spectrometry of Glycosides

  • Marco Brito-Arias
Chapter
  • 597 Downloads

Abstract

High-resolution mass spectrometry has become another valuable tool for characterization of simple and complex glycosides. The method is based on the collision of a high-energy electron against a sample under study producing as result a cation radical fragment known as the molecular ion, which should match with the molecular weight of the molecule. The mass spectrum also registers a number of fragments with the most intense base peak assigned a relative intensity of 100. Mass spectrometry can be applied as high and low ionization experiments, the former the most suitable for glycosides electron impact and the latter for fast atom bombardment (FAB) and electrospray ionization routine experiments for characterization of glycosides. In terms of sensitivity of the measurement this instrumental method requires a small amount of sample, even in the order of nanogram quantities.

Keywords

Fast Atom Bombardment Collisional Activation Fast Atom Bombardment Mass Spectrum Soft Ionization Method Internal Energy Transfer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Marco Brito-Arias
    • 1
  1. 1.Unidad Profesional Interdisciplinaria de Biotecnología Instituto Politécnico Nacional (UPIBI-IPN) Avenida Acueducto s/n Colonia La Laguna TicománCiudad de MéxicoMéxico

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