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Investigating the Swimming of Microbial Pathogens Using Digital Holography

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Biophysics of Infection

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 915))

Abstract

To understand much of the behaviour of microbial pathogens, it is necessary to image living cells, their interactions with each other and with host cells. Species such as Escherichia coli are difficult subjects to image: they are typically microscopic, colourless and transparent. Traditional cell visualisation techniques such as fluorescent tagging or phase-contrast microscopy give excellent information on cell behaviour in two dimensions, but no information about cells moving in three dimensions. We review the use of digital holographic microscopy for three-dimensional imaging at high speeds, and demonstrate its use for capturing the shape and swimming behaviour of three important model pathogens: E. coli, Plasmodium spp. and Leishmania spp.

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Notes

  1. 1.

    Although, intriguingly, not yet among the archaea (Cavicchioli et al. 2003).

  2. 2.

    Eukaryotic flagella and motile cilia are structurally very similar; their names appear to be used somewhat interchangeably within the motility literature.

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Authors and Affiliations

  1. Department of Physics, University of York, Heslington, York, YO10 5DD, England

    K. L. Thornton, R. C. Findlay & L. G. Wilson

  2. Centre for Immunology and Infection, Department of Biology, University of York, Heslington, York, YO10 5DD, England

    R. C. Findlay & P. B. Walrad

Authors
  1. K. L. Thornton
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  2. R. C. Findlay
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  3. P. B. Walrad
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  4. L. G. Wilson
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Corresponding author

Correspondence to L. G. Wilson .

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Editors and Affiliations

  1. Biological Physical Sciences Institute, University of York, York, United Kingdom

    Mark C. Leake

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Thornton, K.L., Findlay, R.C., Walrad, P.B., Wilson, L.G. (2016). Investigating the Swimming of Microbial Pathogens Using Digital Holography. In: Leake, M. (eds) Biophysics of Infection. Advances in Experimental Medicine and Biology, vol 915. Springer, Cham. https://doi.org/10.1007/978-3-319-32189-9_3

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