Skip to main content

Genome-Wide Analysis of DNA Methylation Patterns by High-Throughput Sequencing

  • Chapter
  • First Online:
Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing

Abstract

Epigenetic modifications of chromatin and DNA are relevant for eukaryotic gene expression. DNA methylation is the paradigm epigenetic modification that is associated with transcriptional repression. Perturbations of DNA methylation patterns are frequently associated with cancer and aging, raising a great interest in understanding the contribution of this mark to human health. High-throughput sequencing allows interrogating the status of methylated DNA at nucleotide resolution and genome-wide, bringing unprecedented views on the distribution and dynamics of this relevant modification in healthy and diseased tissues. Here we discuss commonly used wet-lab methodologies and computational approaches to identify DNA methylation patterns and measure their dynamics during biological processes in a quantitative and unbiased manner.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Altuna Akalin Ph.D. .

Editor information

Editors and Affiliations

Annex: Quick Reference Guide

Annex: Quick Reference Guide

Fig. QG9.1
figure a

Representation of the wet-lab procedure workflow

Fig. QG9.2
figure b

Main steps of the computational analysis pipeline

Table QG9.1 Experimental design considerations
Table QG9.2 Available software recommendations

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer International Publishing Switzerland

About this chapter

Cite this chapter

Baubec, T., Akalin, A. (2016). Genome-Wide Analysis of DNA Methylation Patterns by High-Throughput Sequencing. In: Aransay, A., Lavín Trueba, J. (eds) Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing. Springer, Cham. https://doi.org/10.1007/978-3-319-31350-4_9

Download citation

Publish with us

Policies and ethics