Development of the Biochemical Synthesis for Practical Applications

Abstract

This chapter includes the results of our works fulfilled with an aim to solve the three tasks essential for the further researches of the biological effects of silver nanoparticles, namely; (1) determination of the nanoparticle concentration in micellar solution, (2) preparation of micellar solutions with high nanoparticle concentrations and (3) preparation of solutions containing small-sized nanoparticles (below 20 nm) with narrow distribution for the studies of their size effects on biological objects. These tasks were solved, respectively, by (1) determination of the nanoparticles’ extinction coefficient in micellar solution (2) studies on the influence of metal ions/flavonoid concentration ratio on the yield of nanoparticles and (3) investigation of the influence of silver salt and stabilizer concentrations and that of hydration extent on the mean size and size distribution width of the nanoparticles.