Staining Protocols

  • Alexander E. KalyuzhnyEmail author
Part of the Techniques in Life Science and Biomedicine for the Non-Expert book series (TLSBNE)


Due to the inherent nature of different types of histological tissues composing the body as well as the complexity of tissue antigens, there are no universal “one-size-fits-all” staining protocols in IHC: the approach that works for a particular type of tissue and “to-be detected” antigens may be completely ineffective for other types of tissue and antigens. That is why the most critical step in IHC is the optimization of a staining protocol allowing for the best detection of tissue antigens.


Staining Protocol Nonspecific Staining Tissue Antigen Main Color Endogenous Biotin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  1. 1.University of MinnesotaMinneapolisUSA

Personalised recommendations