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Preparing Tissues for IHC Staining

  • Alexander E. KalyuzhnyEmail author
Chapter
Part of the Techniques in Life Science and Biomedicine for the Non-Expert book series (TLSBNE)

Abstract

Fresh histological tissues cannot be used “as is” and must undergo special processing to turn them into a suitable experimental material: tissues need to be first chemically fixed and then cut into thin sections using special histological instruments. Fixation and tissue sectioning are critical to the success of IHC staining. If they are not done correctly, staining quality can be badly compromised regardless of the use of high-quality primary and secondary antibodies, buffers, and detection reagents.

Keywords

Tissue Section Protein Antigen Tissue Antigen Optimal Cutting Temperature Perfusion Fixation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  1. 1.University of MinnesotaMinneapolisUSA

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