Preparing Tissues for IHC Staining

  • Alexander E. KalyuzhnyEmail author
Part of the Techniques in Life Science and Biomedicine for the Non-Expert book series (TLSBNE)


Fresh histological tissues cannot be used “as is” and must undergo special processing to turn them into a suitable experimental material: tissues need to be first chemically fixed and then cut into thin sections using special histological instruments. Fixation and tissue sectioning are critical to the success of IHC staining. If they are not done correctly, staining quality can be badly compromised regardless of the use of high-quality primary and secondary antibodies, buffers, and detection reagents.


Tissue Section Protein Antigen Tissue Antigen Optimal Cutting Temperature Perfusion Fixation 
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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  1. 1.University of MinnesotaMinneapolisUSA

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