Ancillary Reagents for IHC

  • Alexander E. KalyuzhnyEmail author
Part of the Techniques in Life Science and Biomedicine for the Non-Expert book series (TLSBNE)


Although primary and secondary antibodies are essential for IHC procedure, they cannot be used alone and require ancillary reagents: buffer solutions, antibody diluents, and mounting media. Without them, IHC staining cannot be successfully started and completed. Let us go over each of them and discuss their role in IHC procedure.


Wash Buffer Block Buffer Tissue Antigen Epitope Retrieval Endogenous Enzyme 
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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  1. 1.University of MinnesotaMinneapolisUSA

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