Abstract
Bioethanol is an important biofuel produced by the fermentation of sugars that, together with biodiesel, biobutanol, biomethane, and biohydrogen, composes the range of alternatives to the fossil fuels. Soybean molasses is the main by-product generated at the industrial processing of soybean to produce the soy protein concentrate. It is a rich source of carbohydrates, proteins, lipids, and minerals that could be used as a fermentation medium for microbial growth. The aim of this chapter is to describe studies on the development of processes to produce bioethanol from soybean molasses, focusing on the use of different microorganisms, fermentation scales, and pretreatment strategies. Saccharomyces cerevisiae and Zymomonas mobilis presented interesting ethanol yields and productivities at laboratory scale; however, considering the adequateness to industrial facilities, only the yeast-based process was scaled-up. At pilot scale, an average ethanol yield of 44.13 % over the total initial sugars was achieved, which represented an ethanol yield of 129.2 kg, or 163.6 L of absolute ethanol per ton of dry molasses. The average productivity for the pilot scale fed-batch process was 7.882 g/Lh. After scale-up to an industrial plant, one ton of soybean molasses (dry basis) yielded 162.7 L of absolute ethanol and 3.729 tons of vinasse, a by-product containing 19.5 % solids that had to be concentrated to be employed as an energy source. The pretreatment of soybean molasses by acid and enzymatic hydrolyses provided increases in the ethanol yield over total initial sugars to 62 and 68 %, respectively, and reduced the concentration of residual sugars.
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Abbreviations
- BOD:
-
Biochemical oxygen demand
- Disac:
-
Disaccharides
- FAT:
-
Fundação André Tosello (André Tosello Foundation)
- Fru:
-
Fructose
- Gal:
-
Galactose
- Glu:
-
Glucose
- NRRL:
-
Northern Regional Research Laboratory
- Raf:
-
Raffinose
- SPC:
-
Soy protein concentrate
- Sta:
-
Stachyose
- T. S.:
-
Total sugars
- UFPR:
-
Universidade Federal do Paraná (Federal University of Paraná)
- S :
-
Substrate
- X :
-
Biomass
- P :
-
Product
- r s :
-
Substrate consumption rate
- r x :
-
Biomass production rate
- r p :
-
Product formation rate
- Y X/S :
-
Biomass yield from substrate
- Y P/S :
-
Product yield from substrate
References
Cherubini F (2010) The biorefinery concept: using biomass instead of oil for producing energy and chemicals. Energy Convers Manage 12:1412–1421
Chimilovski JS, Habu S, Teixeira RFB, Thomaz-Soccol V, Noseda MD, Pandey A, Soccol CR (2011) Antitumoral activities of exopolysaccharide of Grifola frondosa produced by submerged fermentation using sugarcane and soybean molasses as carbon source. Food Technol Biotech 49:359–363
Dlugokenski REF, Sella SRBR, Guizelini BP, Vandenberghe LPS, Woiciechowski AL, Soccol CR, Minozzo JC (2011) Use of soybean vinasses as a germinant medium for a Geobacillus stearothermophilus ATCC 7953 sterilization biological indicator. Appl Microbiol Biot 90:713–719
Endres JG (2001) Soy protein products: characteristics, nutritional aspects and utilization. American Oil Chemists’ Society Press, Champaign
Hettiarachchy N, Kalapathy U (1997) Soybean protein products. In: Liu K (ed) Soybeans: chemistry, technology and utilization. Aspen Publishers, Gaithersburg, pp 379–411
IBGE (2015) Levantamento Sistemático da Produção Agrícola. ftp://ibge.gov.br/Producao_Agricola/Levantamento_Sistematico_da_Producao_Agricola_[mensal]/Fasciculo/lspa_201508.pdf Accessed 08 Oct 2015
Karp SG, Igashiyama AH, Siqueira PF, Carvalho JC, Vandenberghe LPS, Thomaz-Soccol V, Coral J, Tholozan JL, Pandey A, Soccol CR (2011) Application of the biorefinery concept to produce L-lactic acid from the soybean vinasse at laboratory and pilot scale. Bioresource Technol 102:1765–1772
Letti LAJ (2007) Production of bioethanol by soybean molasses fermentation by Zymomonas mobilis. Dissertation, Federal University of Paraná/University of Provence
Letti LAJ, Karp SG, Woiciechowski AL, Soccol CR (2012) Ethanol production from soybean molasses by Zymomonas mobilis. Biomass Bioenergy 44:80–86
Long CC, Gibbons WR (2013) Conversion of soy molasses, soy solubles, and dried soybean carbohydrates into ethanol. IJABE 6:62–68
Lusas EW, Riaz MN (1995) Soy protein products: processing and use. J Nutr 125:573–580
Melo AL, Soccol VT, Soccol CR, Nogueira Júnior M (2009) Evaluation of Bacillus sphaericus bioinsecticide produced with white soybean meal as culture medium for the control of Culex (Culex) quinquefasciatus. Cad Saúde Pública 25:563–569
Navarro AR, Sepúlveda MC, Rubio MC (2000) Bio-concentration of vinasse from the alcoholic fermentation of sugar cane molasses. Waste Manage 20:581–585
Peisker M (2001) Manufacturing of soy protein concentrate for animal nutrition. Cahiers Options Mediterraneennes 54:103–107
Prasad S, Singh A, Joshi HC (2007) Ethanol as an alternative fuel from agricultural, industrial and urban residues. Resour Conserv Recy 50:1–39
Rojas MJ, Siqueira PF, Miranda LC, Tardioli PW, Giordano RLC (2014) Sequential proteolysis and cellulolytic hydrolysis of soybean hulls for oligopeptides and ethanol production. Ind Crop Prod 61:201–210
Romão BB, Silva FB, Resende MM, Cardoso VL (2012) Ethanol production from hydrolyzed soybean molasses. Energy Fuel 26:2310–2316
Roukas T (1996) Ethanol production from non-sterilized beet molasses by free and immobilized Saccharomyces cerevisiae cells using fed-batch culture. J Food Eng 27:87–96
Sanada CTN, Karp SG, Spier MR, Portella AC, Gouvêa PM, Yamaguishi CT, Vandenberghe LPS, Pandey A, Soccol CR (2009) Utilization of soybean vinasse for α-galactosidase production. Food Res Int 42:476–483
Silva FB, Romão BB, Cardoso VL, Coutinho Filho U, Ribeiro EJ (2012) Production of ethanol from enzymatically hydrolyzed soybean molasses. Biochem Eng J 69:61–68
Siqueira PF (2007) Production of bio-ethanol from soybean molasses by Saccharomyces cerevisiae. Dissertation, Federal University of Paraná
Siqueira PF, Karp SG, Carvalho JC, Sturm W, Rodríguez-León JA, Tholozan JL, Singhania RR, Pandey A, Soccol CR (2008) Production of bio-ethanol from soybean molasses by Saccharomyces cerevisiae at laboratory, pilot and industrial scales. Bioresource Technol 99:8156–8163
Soccol CR, Vandenberghe LPS, Costa B, Woiciechowski AL, Carvalho JC, Medeiros ABP, Francisco AM, Bonomi LJ (2005) Brazilian biofuel program: an overview. J Sci Ind Res 64:897–904
Soccol CR, Karp SG, Noll MA, Soccol VT, Suarez CB, Pandey A (2009) Application of biorefinery concept for soybean industrialization in Brazil. In: Pandey A, Larroche C, Soccol CR, Dussap CG (eds) New horizons in biotechnology. Asiatech Publishers, New Delhi, pp 186–207
Soccol CR, Karp SG, Siqueira PF, Sanada CTN, Soccol VT, Pandey A (2013) Biorefinery concept applied to valorization of agro-food coproducts and wastes: integrated process for waste recycling. In: Soccol CR, Pandey A, Larroche C (eds) Fermentation processes engineering in the food industry, 1st edn. CRC Press, New York, pp 430–464
Togrul H, Arslan N (2004) Mathematical model for prediction of apparent viscosity of molasses. J Food Eng 62:281–289
USDA (2015) World agricultural supply and demand estimates. http://www.usda.gov/oce/commodity/wasde/latest.pdf. Accessed 08 Oct 2015
Weingartner V (2010) Produção, purificação e identificação de mananase, obtida por fermentação no estado sólido utilizando cascas de soja e Aspergillus niger. Dissertation, Federal University of Paraná
Zech M, Görisch H (1995) Invertase from Saccharomyces cerevisiae: reversible inactivation by components of industrial molasses media. Enzyme Microb Tech 17:41–46
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Karp, S.G., Woiciechowski, A.L., Letti, L.A.J., Soccol, C.R. (2016). Bioethanol from Soybean Molasses. In: Soccol, C., Brar, S., Faulds, C., Ramos, L. (eds) Green Fuels Technology. Green Energy and Technology. Springer, Cham. https://doi.org/10.1007/978-3-319-30205-8_10
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