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Microfluidic Methods for Molecular Biology pp 205–218Cite as

Microfluidic Paper-Based Multiplexing Biosensors for Electrochemical Detection of Metabolic Biomarkers

Abstract

Paper-based microfluidic devices, which have emerged as a low-cost analytical platform technology, promise simple yet powerful solutions for point-of-care (POC) disease diagnostics. In this chapter, we present a microfluidic paper-based electrochemical biosensor array capable of multiplexed detection of physiologically related metabolic markers. Compared with existing paper-based electrochemical devices, our design consists of a multiplexing array of eight electrochemical biosensors and works together with a portable, custom-made potentiostat for signal readout. The integrated platform, including the paper-based biosensor array and the portable potentiostat, is able to detect multiple target markers from one sample and carry out multiple measurements for each marker. Based on this platform, we conduct the detection of three metabolic biomarkers (i.e., glucose, lactate, and uric acid) in artificial urine, and achieve analytical performance comparable to existing commercial and paper-based analytical devices. As an integrated electrochemical system, the proposed multiplexing biosensor array will permit rapid and inexpensive disease diagnosis at the POC and/or in resource-limited regions.

Keywords

  • Paper-based microfluidics
  • Biosensor
  • Electrochemical detection
  • Multiplexing
  • Metabolic markers
  • Point-of-care diagnostics

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Author information

Authors and Affiliations

  1. Department of Mechanical Engineering, McGill University, 817 Sherbrooke Street West, MD270, Montreal, QC, H3A 0C3, Canada

    Chen Zhao & Xinyu Liu Ph.D.

  2. Department of Materials Science and Engineering, Iowa State University, 2220A Hoover, Ames, IA, 50011, USA

    Martin M. Thuo Ph.D.

Authors
  1. Chen Zhao
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  2. Martin M. Thuo Ph.D.
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  3. Xinyu Liu Ph.D.
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Corresponding author

Correspondence to Xinyu Liu Ph.D. .

Editor information

Editors and Affiliations

  1. Virginia Tech, Blacksburg, Virginia, USA

    Chang Lu

  2. Dpt of Biomedical Engg. & Mechanics, Virginia Tech, Blacksburg, Virginia, USA

    Scott S. Verbridge

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Zhao, C., Thuo, M.M., Liu, X. (2016). Microfluidic Paper-Based Multiplexing Biosensors for Electrochemical Detection of Metabolic Biomarkers. In: Lu, C., Verbridge, S. (eds) Microfluidic Methods for Molecular Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-30019-1_10

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