Modern Approaches in Fascioloides magna Studies

  • Ivica Králová-HromadováEmail author
  • Ludmila Juhásová
  • Eva Bazsalovicsová
Part of the SpringerBriefs in Animal Sciences book series (BRIEFSANIMAL)


The methods of cellular and molecular biology represent useful and attractive tools that have been applied in identification, taxonomy and systematics of broad spectrum of parasitic organisms over the past decades. The pilot molecular data on Fascioloides magna appeared in 90s of the 20th century. After more than 20 years of molecular and cellular research of F. magna, effective markers for accurate species identification and large-scale population studies, detailed subcellular structure of the parasite, and immunologically active molecules, were detected. This chapter is divided into four sections. First one is dealing with general structure and characterization of ribosomal genes and their utilization in molecular taxonomy and phylogeny of F. magna. Second part is focused on characterization and structure of mitochondrial genes and their application in studies on genetic interrelationships, biogeography, origin and transmission routes of F. magna. Microsatellites, biparentally inherited multilocus markers, are useful population genetics markers described in third subchapter. Data on ultrastructure, karyotype and chromosomal location of ribosomal genes of F. magna are presented in the last part of this chapter. In addition, we provided brief overview on current knowledge of F. magna isoenzyme analyses, excretory/secretory proteins, humoral immune responses during experimental infection with F. magna in selected final hosts, and up to date technologies of transcriptome analysis.


Giant liver fluke Ribosomal DNA Mitochondrial DNA Microsatellites Karyotype Transcriptome Excretory/secretory proteins Isoenzyme analysis Molecular taxonomy Phylogeny 


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© The Author(s) 2016

Authors and Affiliations

  • Ivica Králová-Hromadová
    • 1
    Email author
  • Ludmila Juhásová
    • 1
  • Eva Bazsalovicsová
    • 1
  1. 1.Institute of ParasitologySlovak Academy of SciencesKošiceSlovakia

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