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Ocular Drug Delivery: Impact of In Vitro Cell Culture Models

  • Aum Solanki
  • Sumir Desai
  • Aditya Grover
  • Anjali Hirani
  • Yashwant PathakEmail author
  • Vijaykumar SutariyaEmail author
Chapter

Abstract

The physiological characteristics make effective drug delivery very challenging. Various structures such as the cornea, conjunctival epithelium, blood-retinal barrier (BRB), and the retinal epithelium block or severely reduce the concentration of drugs that can reach particular locations in the eye. Because of this, in vitro models that can effectively model each of these barriers are highly desirable. Such in vitro modeling allows researchers to minimize the use of animal studies, as drug delivery experiments require frequent euthanization. As a substitute for animal experiments, various types of in vitro models have been developed that are made up of primary cell cultures or immortalized cell lines. These cell lines allow for the detailed study of the individual processes that determine the ability of drugs and drug delivery systems to reach their desired locations in therapeutic concentrations. This chapter discusses various examples of such cell lines and their applicability in various drug delivery studies.

Keywords

In vitro modeling Corneal epithelium Corneal stroma Conjunctival epithelium Blood-retinal barrier (BRB) Retinal pigment epithelium (RPE) Tight junctions Primary cell culture Immortalized cell culture 

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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  1. 1.Honors CollegeUniversity of South FloridaTampaUSA
  2. 2.College of PharmacyUniversity of South FloridaTampaUSA
  3. 3.College of MedicineUniversity of South FloridaTampaUSA
  4. 4.Department of Pharmaceutical SciencesUSF College of Pharmacy, University of South FloridaTampaUSA
  5. 5.School of Biomedical Engineering and SciencesVirginia Tech-Wake Forest UniversityBlacksburgUSA
  6. 6.Department of Pharmaceutical SciencesCollege of Pharmacy, University of South FloridaTampaUSA

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